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A9W732 (A9W732_METEP) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
EC=2.1.1.33 HAMAP-Rule MF_01057
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase HAMAP-Rule MF_01057
tRNA(m7G46)-methyltransferase HAMAP-Rule MF_01057
Gene names
Name:trmB HAMAP-Rule MF_01057
Ordered Locus Names:Mext_3307
OrganismMethylobacterium extorquens (strain PA1) [Complete proteome] [HAMAP] EMBL ABY31694.1
Taxonomic identifier419610 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium ›

Protein attributes

Sequence length245 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the methyltransferase superfamily. TrmB family. HAMAP-Rule MF_01057

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region221 – 2244Substrate binding By similarity HAMAP-Rule MF_01057

Sites

Binding site731S-adenosyl-L-methionine By similarity HAMAP-Rule MF_01057
Binding site981S-adenosyl-L-methionine By similarity HAMAP-Rule MF_01057
Binding site1251S-adenosyl-L-methionine By similarity HAMAP-Rule MF_01057
Binding site1471S-adenosyl-L-methionine By similarity HAMAP-Rule MF_01057
Binding site1511Substrate By similarity HAMAP-Rule MF_01057
Binding site1831Substrate By similarity HAMAP-Rule MF_01057

Sequences

Sequence LengthMass (Da)Tools
A9W732 [UniParc].

Last modified February 5, 2008. Version 1.
Checksum: C734012BA7C1E11D

FASTA24527,533
        10         20         30         40         50         60 
MMGRDDDIPP ETGGTAETER AFYGRRKGKR LRPGQEQRLE GALPSLRVTL PDRGEFLDPP 

        70         80         90        100        110        120 
SLFPVPVDEV WLEIGFGGGE HLAAQAEAHR QAGIIGCEPF VNGVVKLLRA VDERDLRNVR 

       130        140        150        160        170        180 
VWDEDATALL AALPDASLAR VYLLYPDPWP KRRQRKRRFV SDASLAEIAR VLRPGGHFRF 

       190        200        210        220        230        240 
ASDIDDYVGW TLVRAARCPQ LRWTAQSADD WRKPFEGWPG TRYEAKALAA GRLPSYLTFE 


RVGAD

« Hide

References

[1]"Complete sequence of Methylobacterium extorquens PA1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Kim E., Marx C., Richardson P.
Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PA1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000908 Genomic DNA. Translation: ABY31694.1.
RefSeqYP_001640765.1. NC_010172.1.

3D structure databases

ProteinModelPortalA9W732.
ModBaseSearch...

Protein-protein interaction databases

STRING419610.Mext_3307.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABY31694; ABY31694; Mext_3307.
GeneID5833570.
KEGGmex:Mext_3307.
PATRIC22537214. VBIMetExt98426_3354.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073969.
KOK03439.
OMALYPDPWP.
ProtClustDBCLSK2331650.

Enzyme and pathway databases

BioCycMEXT419610:GI32-3359-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA9W732_METEP
AccessionPrimary (citable) accession number: A9W732
Entry history
Integrated into UniProtKB/TrEMBL: February 5, 2008
Last sequence update: February 5, 2008
Last modified: May 1, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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