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Protein

Tetraacyldisaccharide 4'-kinase

Gene

lpxK

Organism
Methylobacterium extorquens (strain PA1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA).UniRule annotation

Catalytic activityi

ATP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-4-O-phospho-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate).UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 6 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi52 – 59ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processLipid A biosynthesis, Lipid biosynthesis, Lipid metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00359; UER00482.

Names & Taxonomyi

Protein namesi
Recommended name:
Tetraacyldisaccharide 4'-kinaseUniRule annotation (EC:2.7.1.130UniRule annotation)
Alternative name(s):
Lipid A 4'-kinaseUniRule annotation
Gene namesi
Name:lpxKUniRule annotation
Ordered Locus Names:Mext_2969
OrganismiMethylobacterium extorquens (strain PA1)
Taxonomic identifieri419610 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000008546 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001347441 – 327Tetraacyldisaccharide 4'-kinaseAdd BLAST327

Interactioni

Protein-protein interaction databases

STRINGi419610.Mext_2969.

Structurei

3D structure databases

SMRiA9W6S6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxK family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CHD. Bacteria.
COG1663. LUCA.
HOGENOMiHOG000004954.
KOiK00912.
OMAiMDDGFQN.
OrthoDBiPOG091H000L.

Family and domain databases

HAMAPiMF_00409. LpxK. 1 hit.
InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR003758. Tetraacyldisaccharide_4-kinase.
PANTHERiPTHR42724. PTHR42724. 1 hit.
PfamiView protein in Pfam
PF02606. LpxK. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00682. lpxK. 1 hit.

Sequencei

Sequence statusi: Complete.

A9W6S6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPPGFWSRP PTHPLARLLA PAGRVYGGLT ANRMDRPGAT PPCPVLCVGN
60 70 80 90 100
FTLGGAGKTP TALSLVRLLR ELGRTPALLS RGYGGRLAGP LVVDPARHAA
110 120 130 140 150
AEVGDEPLLL AQAAPTIVAR DRPAGARLCA ASGADVIVMD DGLQNPSLTK
160 170 180 190 200
TLSLAVVDGG AGLGNGLPFP AGPLRAPLAR QWPHVAGLVL VGEGGPGEAM
210 220 230 240 250
AAEAERRGLP VHRARLVPET GSDWAGRRVV AFAGIGRPQK FFETLRSLGA
260 270 280 290 300
EIVAEGAFPD HHPYRPGDWT ALSALAAREG ASLVTTEKDA VRLPAEARAV
310 320
VDVLRVTLAF ANETRLRQQL AAAFPHA
Length:327
Mass (Da):34,027
Last modified:February 5, 2008 - v1
Checksum:i9CC9A98D52106623
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000908 Genomic DNA. Translation: ABY31358.1.
RefSeqiWP_012254296.1. NC_010172.1.

Genome annotation databases

EnsemblBacteriaiABY31358; ABY31358; Mext_2969.
KEGGimex:Mext_2969.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiLPXK_METEP
AccessioniPrimary (citable) accession number: A9W6S6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 5, 2008
Last modified: June 7, 2017
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families