A9W6S6 (LPXK_METEP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetraacyldisaccharide 4'-kinase
EC=2.7.1.130

Alternative name(s):
Lipid A 4'-kinase

Gene names

Name:	lpxK
Ordered Locus Names:	Mext_2969

Organism

Methylobacterium extorquens (strain PA1) [Complete proteome] [HAMAP]

Taxonomic identifier

419610 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA) By similarity. HAMAP MF_00409
Catalytic activity	ATP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate). HAMAP MF_00409
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6. HAMAP MF_00409
Sequence similarities	Belongs to the LpxK family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tetraacyldisaccharide 4'-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Tetraacyldisaccharide 4'-kinase HAMAP MF_00409		PRO_1000134744
Regions
	Nucleotide binding	52 – 59	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A9W6S6 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 9CC9A98D52106623	FASTA	327	34,027
	10 20 30 40 50 60 MRPPGFWSRP PTHPLARLLA PAGRVYGGLT ANRMDRPGAT PPCPVLCVGN FTLGGAGKTP 70 80 90 100 110 120 TALSLVRLLR ELGRTPALLS RGYGGRLAGP LVVDPARHAA AEVGDEPLLL AQAAPTIVAR 130 140 150 160 170 180 DRPAGARLCA ASGADVIVMD DGLQNPSLTK TLSLAVVDGG AGLGNGLPFP AGPLRAPLAR 190 200 210 220 230 240 QWPHVAGLVL VGEGGPGEAM AAEAERRGLP VHRARLVPET GSDWAGRRVV AFAGIGRPQK 250 260 270 280 290 300 FFETLRSLGA EIVAEGAFPD HHPYRPGDWT ALSALAAREG ASLVTTEKDA VRLPAEARAV 310 320 VDVLRVTLAF ANETRLRQQL AAAFPHA

References

[1]

"Complete sequence of Methylobacterium extorquens PA1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Marx C., Richardson P. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PA1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000908 Genomic DNA. Translation: ABY31358.1.
RefSeq	YP_001640429.1. NC_010172.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A9W6S6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5835565.
GenomeReviews	Gene locus Mext_2969 in contig CP000908_GR.
KEGG	mex:Mext_2969.
PATRIC	22536512. VBIMetExt98426_3005.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG663914.
OMA	VILMDDG.
ProtClustDB	CLSK2331442.
Enzyme and pathway databases
BioCyc	MEXT419610:MEXT_2969-MONOMER.
Family and domain databases
HAMAP	MF_00409. LpxK. [Tree]
InterPro	IPR003758. Tetraacyldisaccharide_4-kinase. [Graphical view]
KO	K00912.
Pfam	PF02606. LpxK. 1 hit. [Graphical view]
TIGRFAMs	TIGR00682. LpxK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXK_METEP

Accession

Primary (citable) accession number: A9W6S6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program