A9W5W4 (A9W5W4_METEP) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA gyrase subunit B RuleBase RU003363 HAMAP MF_01898
EC=5.99.1.3 RuleBase RU003363 HAMAP MF_01898

Gene names

Name:	gyrB HAMAP MF_01898
Ordered Locus Names:	Mext_2577

Organism

Methylobacterium extorquens (strain PA1) [Complete proteome] [HAMAP]

Taxonomic identifier

419610 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	815 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. RuleBase RU003363 HAMAP MF_01898
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. SAAS SAAS002288 RuleBase RU003363 HAMAP MF_01898
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer By similarity. HAMAP MF_01898
Subcellular location	Cytoplasm By similarity HAMAP MF_01898.
Sequence similarities	Belongs to the type II topoisomerase family. HAMAP MF_01898 RuleBase RU000380

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP MF_01898
Ligand	ATP-binding SAAS SAAS002288 HAMAP MF_01898 RuleBase RU000380 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS002288 HAMAP MF_01898 RuleBase RU000380
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A9W5W4 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 1FDF3366CC4D8BA6	FASTA	815	89,569
	10 20 30 40 50 60 MADSPNTEHA DSYGAESIRV LKGLDAVRKR PGMYIGDTDD GSGLHHMIYE VVDNAIDEAL 70 80 90 100 110 120 AGHADLVTVT LNADGSVTVS DNGRGIPTDI HREEGVSAAE VIMTQLHAGG KFDQNSYKVS 130 140 150 160 170 180 GGLHGVGVSV VNALSTSLKL RIWRGGKEHR MEFRHGDAVA PLEVVGPAGD RRGTEVTFLP 190 200 210 220 230 240 STETFTMTEF DYPTLEKRLR ELAFLNSGVR IVVTDARHAE HKREELCYEG GVEAFVRYLD 250 260 270 280 290 300 RSRKPVDGMT KPVTVLGERD GIRVEVALWW NDSFNETVLP FTNNIPQRDG GTHMAGFRAA 310 320 330 340 350 360 LTRQLTGYAE SSGIAKREKV SLTGDDCREG LTAVISVQVP DPKFSSQTKD KLVSSEVRPA 370 380 390 400 410 420 VENILNEGLS TWLEENPAQA KSVMGKVVLA AAAREAARKA RETITRKGAL DIASLPGKLA 430 440 450 460 470 480 DCQERDPSKC ELLLVEGDSA GGSAKQGRDR TFQAVLPLRG KILNVERVRA DRMLSSAEIG 490 500 510 520 530 540 TLITALGAGI GRSSTDREGF NPDKLRYHRI IIMTDADVDG SHIRTLLLTF FFRQMPELIE 550 560 570 580 590 600 RGHLYIAQPP LYKAERGRRV LYLKDERALE DYLIDQGVEG AVLRLASGTE FGGAQLKTLV 610 620 630 640 650 660 EEARAFRGIL HGLHTRYDRS VVEQSVLAGA FAKDVAENLG EAEVLADRTA KRLDRIADEI 670 680 690 700 710 720 EKGWTGAASE GGYVFSRTLR SVTQVANLDA TLLSSQEARR LAERAETLRE IYEEPVTLAR 730 740 750 760 770 780 KGDETELYGP VGLFEAVMAF GRKGLQLQRY KGLGEMTAQQ LWETTLDRDV RSLLQVKVKD 790 800 810 TTDADDLFVK LMGDVVEPRR EFIQENALSV ANLDV

References

[1]

"Complete sequence of Methylobacterium extorquens PA1." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Marx C., Richardson P. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PA1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000908 Genomic DNA. Translation: ABY30970.1.
RefSeq	YP_001640041.1. NC_010172.1.
3D structure databases
ProteinModelPortal	A9W5W4.
SMR	A9W5W4. Positions 18-388.
ModBase	Search...
Protein-protein interaction databases
STRING	A9W5W4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5834857.
GenomeReviews	Gene locus Mext_2577 in contig CP000908_GR.
KEGG	mex:Mext_2577.
PATRIC	22535694. VBIMetExt98426_2600.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG304889.
OMA	DCSSRDP.
ProtClustDB	PRK14939.
Family and domain databases
HAMAP	MF_01898. GyrB. [Tree]
InterPro	IPR003594. ATPase-like_ATP-bd. IPR011557. GyrB. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR001241. Topo_IIA. IPR013759. Topo_IIA_B/N_ab. IPR002288. Topo_IIA_B_C. IPR013506. Topo_IIA_bsu_dom2. IPR013760. Topo_IIA_cen. IPR018522. TopoIIA_CS. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.40.50.670. Topo_IIA_B/N_ab. 2 hits.
KO	K02470.
Pfam	PF00204. DNA_gyraseB. 1 hit. PF00986. DNA_gyraseB_C. 1 hit. PF02518. HATPase_c. 1 hit. [Graphical view]
PRINTS	PR00418. TPI2FAMILY.
SMART	SM00387. HATPase_c. 1 hit. SM00433. TOP2c. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01059. GyrB. 1 hit.
PROSITE	PS00177. TOPOISOMERASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A9W5W4_METEP

Accession

Primary (citable) accession number: A9W5W4

Entry history

Integrated into UniProtKB/TrEMBL:	February 5, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)