A9W4H0 (LPXD_METEP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	Mext_2080

Organism

Methylobacterium extorquens (strain PA1) [Complete proteome] [HAMAP]

Taxonomic identifier

419610 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_1000127683
Sites
	Active site	257	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9W4H0 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 3976A4E6157FA912	FASTA	351	35,785
	10 20 30 40 50 60 MSDPVFIAPK GGLTLGSVAE ACGVPLPDGA DPAQPVTGAA PLETAGPSEL AYMDNARYGD 70 80 90 100 110 120 ALATTRALAC LVSPRFAPRV PAGTIALVTR DPYRAYAGLL ARLYEEAMRP GSLFAAAGVS 130 140 150 160 170 180 PGAHVHPQAR LEDGMRIDPG AVVGPGAEIG SGTVLGPNAV IGPNVRIGRD CSIGAGATLT 190 200 210 220 230 240 HALVGNRVIV HPGARIGQDG FGFAMGAGGH IKVPQVGRVI IQDDVEIGAN TTIDRGASRD 250 260 270 280 290 300 TVVGEGTKID NLVQIAHNVV IGRHCVIVSG VGISGSTTLE DYVVLGGQVG VVGHLRIGMG 310 320 330 340 350 SQIAGSSNVN RDVPPGSRWG GTPAKPVRTW FREMTTLARL AERGGKDEAE G

References

[1]

"Complete sequence of Methylobacterium extorquens PA1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Marx C., Richardson P. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PA1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000908 Genomic DNA. Translation: ABY30476.1.
RefSeq	YP_001639547.1. NC_010172.1.
3D structure databases
ProteinModelPortal	A9W4H0.
ModBase	Search...
Protein-protein interaction databases
STRING	A9W4H0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5832790.
GenomeReviews	Gene locus Mext_2080 in contig CP000908_GR.
KEGG	mex:Mext_2080.
PATRIC	22534667. VBIMetExt98426_2092.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG469615.
OMA	VQIGRVI.
ProtClustDB	PRK00892.
Enzyme and pathway databases
BioCyc	MEXT419610:MEXT_2080-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 4 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_METEP

Accession

Primary (citable) accession number: A9W4H0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program