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Protein

Malate dehydrogenase

Gene

mdh

Organism
Methylobacterium extorquens (strain PA1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei34 – 341NADUniRule annotation
Binding sitei83 – 831SubstrateUniRule annotation
Binding sitei89 – 891SubstrateUniRule annotation
Binding sitei96 – 961NADUniRule annotation
Binding sitei121 – 1211SubstrateUniRule annotation
Binding sitei152 – 1521SubstrateUniRule annotation
Active sitei176 – 1761Proton acceptorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 156NADUniRule annotation
Nucleotide bindingi119 – 1213NADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMEXT419610:GI32-1682-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:Mext_1643
OrganismiMethylobacterium extorquens (strain PA1)
Taxonomic identifieri419610 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
ProteomesiUP000008546 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320Malate dehydrogenasePRO_1000126138Add
BLAST

Proteomic databases

PRIDEiA9W386.

Interactioni

Protein-protein interaction databases

STRINGi419610.Mext_1643.

Structurei

Secondary structure

1
320
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 95Combined sources
Helixi13 – 2412Combined sources
Beta strandi29 – 335Combined sources
Beta strandi35 – 384Combined sources
Helixi39 – 5416Combined sources
Beta strandi60 – 656Combined sources
Helixi66 – 694Combined sources
Beta strandi73 – 775Combined sources
Helixi89 – 11022Combined sources
Beta strandi115 – 1184Combined sources
Helixi123 – 13412Combined sources
Helixi138 – 1403Combined sources
Beta strandi141 – 1433Combined sources
Helixi146 – 16116Combined sources
Helixi165 – 1673Combined sources
Beta strandi172 – 1743Combined sources
Helixi177 – 1793Combined sources
Beta strandi180 – 1823Combined sources
Helixi184 – 1863Combined sources
Helixi194 – 1996Combined sources
Helixi205 – 21612Combined sources
Helixi218 – 2269Combined sources
Beta strandi227 – 2293Combined sources
Helixi233 – 24715Combined sources
Beta strandi252 – 26211Combined sources
Helixi263 – 2653Combined sources
Beta strandi267 – 27812Combined sources
Beta strandi281 – 2855Combined sources
Helixi292 – 31423Combined sources
Helixi317 – 3193Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4RORX-ray1.66A1-320[»]
4ROSX-ray1.95A1-320[»]
ProteinModelPortaliA9W386.
SMRiA9W386. Positions 1-314.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 3 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213794.
KOiK00024.
OMAiDIMQANT.
OrthoDBiEOG6091FG.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

A9W386-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARSKIALIG AGQIGGTLAH LAGLKELGDV VLFDIVDGVP QGKALDIAES
60 70 80 90 100
APVDGFDAKY SGASDYSAIA GADVVIVTAG VPRKPGMSRD DLIGINLKVM
110 120 130 140 150
EAVGAGIKEH APDAFVICIT NPLDAMVWAL QKFSGLPTNK VVGMAGVLDS
160 170 180 190 200
ARFRHFLAEE FGVSVEDVTA FVLGGHGDDM VPLTRYSTVA GVPLTDLVKL
210 220 230 240 250
GWTTQEKLDA MVERTRKGGG EIVNLLKTGS AFYAPAASAI AMAESYLRDK
260 270 280 290 300
KRVLPCAAYL DGQYGIDGLY VGVPVVIGEN GVERVLEVTF NDDEKAMFEK
310 320
SVNSVKGLIE ACKSVNDKLA
Length:320
Mass (Da):33,647
Last modified:February 5, 2008 - v1
Checksum:iB2CF23AAA0F032EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000908 Genomic DNA. Translation: ABY30042.1.
RefSeqiWP_003599890.1. NC_010172.1.
YP_001639113.1. NC_010172.1.

Genome annotation databases

EnsemblBacteriaiABY30042; ABY30042; Mext_1643.
KEGGimex:Mext_1643.
PATRICi22533777. VBIMetExt98426_1649.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000908 Genomic DNA. Translation: ABY30042.1.
RefSeqiWP_003599890.1. NC_010172.1.
YP_001639113.1. NC_010172.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4RORX-ray1.66A1-320[»]
4ROSX-ray1.95A1-320[»]
ProteinModelPortaliA9W386.
SMRiA9W386. Positions 1-314.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi419610.Mext_1643.

Proteomic databases

PRIDEiA9W386.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABY30042; ABY30042; Mext_1643.
KEGGimex:Mext_1643.
PATRICi22533777. VBIMetExt98426_1649.

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213794.
KOiK00024.
OMAiDIMQANT.
OrthoDBiEOG6091FG.

Enzyme and pathway databases

BioCyciMEXT419610:GI32-1682-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PA1.

Entry informationi

Entry nameiMDH_METEP
AccessioniPrimary (citable) accession number: A9W386
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 5, 2008
Last modified: May 27, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.