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Protein

Orotidine 5'-phosphate decarboxylase

Gene

pyrF

Organism
Methylobacterium extorquens (strain PA1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP).UniRule annotation

Catalytic activityi

Orotidine 5'-phosphate = UMP + CO2.UniRule annotation

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes UMP from orotate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Orotate phosphoribosyltransferase (pyrE)
  2. Orotidine 5'-phosphate decarboxylase (pyrF)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei16SubstrateUniRule annotation1
Binding sitei38SubstrateUniRule annotation1
Active sitei67Proton donorUniRule annotation1
Binding sitei119SubstrateUniRule annotation1
Binding sitei180SubstrateUniRule annotation1
Binding sitei189SubstrateUniRule annotation1
Binding sitei209Substrate; via amide nitrogenUniRule annotation1
Binding sitei210SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processPyrimidine biosynthesis

Enzyme and pathway databases

BioCyciMEXT419610:G1GAN-1605-MONOMER
UniPathwayiUPA00070; UER00120

Names & Taxonomyi

Protein namesi
Recommended name:
Orotidine 5'-phosphate decarboxylaseUniRule annotation (EC:4.1.1.23UniRule annotation)
Alternative name(s):
OMP decarboxylaseUniRule annotation
Short name:
OMPDCaseUniRule annotation
Short name:
OMPdecaseUniRule annotation
Gene namesi
Name:pyrFUniRule annotation
Ordered Locus Names:Mext_1520
OrganismiMethylobacterium extorquens (strain PA1)
Taxonomic identifieri419610 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000008546 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001385391 – 232Orotidine 5'-phosphate decarboxylaseAdd BLAST232

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi419610.Mext_1520

Structurei

3D structure databases

ProteinModelPortaliA9W2W3
SMRiA9W2W3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni65 – 74Substrate bindingUniRule annotation10

Sequence similaritiesi

Belongs to the OMP decarboxylase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4106EG9 Bacteria
COG0284 LUCA
HOGENOMiHOG000226070
KOiK01591
OMAiNFKIFLD
OrthoDBiPOG091H0256

Family and domain databases

Gene3Di3.20.20.701 hit
HAMAPiMF_01200_B OMPdecase_type1_B, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR014732 OMPdecase
IPR018089 OMPdecase_AS
IPR001754 OMPdeCOase_dom
IPR011060 RibuloseP-bd_barrel
PANTHERiPTHR32119:SF2 PTHR32119:SF2, 1 hit
PfamiView protein in Pfam
PF00215 OMPdecase, 1 hit
SMARTiView protein in SMART
SM00934 OMPdecase, 1 hit
SUPFAMiSSF51366 SSF51366, 1 hit
TIGRFAMsiTIGR01740 pyrF, 1 hit
PROSITEiView protein in PROSITE
PS00156 OMPDECASE, 1 hit

Sequencei

Sequence statusi: Complete.

A9W2W3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPESADPRDR LIVALDLPDV EAAERLVARI GDAATFYKIG YRLAYAGGLD
60 70 80 90 100
FAARLAKSGK KTFLDLKLHD IGNTVEEGVR SASALGATFL TVHAYPQTMR
110 120 130 140 150
AAVRGRGPGL KILAVTVLTS YDDADAAEAG YALPIADLVA RRAEQAAEIG
160 170 180 190 200
IDGIVCSAVE AGAVRHRIGP SGLIVTPGIR PAGAEAGDQK RVMTPGQARA
210 220 230
AGIDHVVVGR PITGADDPRA VAQRIVAEME DA
Length:232
Mass (Da):24,189
Last modified:February 5, 2008 - v1
Checksum:i4BEC02F65D9630A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000908 Genomic DNA Translation: ABY29919.1
RefSeqiWP_012253131.1, NC_010172.1

Genome annotation databases

EnsemblBacteriaiABY29919; ABY29919; Mext_1520
KEGGimex:Mext_1520

Similar proteinsi

Entry informationi

Entry nameiPYRF_METEP
AccessioniPrimary (citable) accession number: A9W2W3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 5, 2008
Last modified: February 28, 2018
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome