A9VX42 (ISPE_METEP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Mext_3109

Organism

Methylobacterium extorquens (strain PA1) [Complete proteome] [HAMAP]

Taxonomic identifier

419610 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000092095
Regions
	Nucleotide binding	97 – 107	11	ATP Potential
Sites
	Active site	11	1	By similarity
	Active site	139	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9VX42 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 4C88CFAF26B8EEDA	FASTA	291	29,248
	10 20 30 40 50 60 MSVLTTRAPA KINLTLHILG RRPGDGYHAL ESLVAFADVA DTLELVPGPD LTLDISGPTA 70 80 90 100 110 120 GPAGPLDDNL VLRAARHLAA GVDGLRLGAF RLHKQLPVAA GIGGGSSDAA AALRLLAELN 130 140 150 160 170 180 GLALDYPAVI AAARATGADV PVCLDPRARM MRGAGEEIGP VLGLASLPAV LVNPGVPVST 190 200 210 220 230 240 APVFKALGLA VGQQLDGAEH PVVGASLDAD AVLGVIAPAR NDLEAPALTV APVIGEALGL 250 260 270 280 290 LWAQAGCRLA RMSGSGATVF AIFSDDGAAE MAAGAIRAAQ PGWWVEPTRL A

References

[1]

"Complete sequence of Methylobacterium extorquens PA1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Marx C., Richardson P. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PA1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000908 Genomic DNA. Translation: ABY31497.1.
RefSeq	YP_001640568.1. NC_010172.1.
3D structure databases
ProteinModelPortal	A9VX42.
ModBase	Search...
Protein-protein interaction databases
STRING	A9VX42.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5835060.
GenomeReviews	Gene locus Mext_3109 in contig CP000908_GR.
KEGG	mex:Mext_3109.
PATRIC	22536798. VBIMetExt98426_3147.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	VSTAEVY.
ProtClustDB	PRK14608.
Enzyme and pathway databases
BioCyc	MEXT419610:MEXT_3109-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_METEP

Accession

Primary (citable) accession number: A9VX42

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program