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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

BcerKBAB4_5300

Organism
Bacillus weihenstephanensis (strain KBAB4)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein predictedi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.SAAS annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).SAAS annotation

Cofactori

Mg2+SAAS annotationNote: Binds 1 Mg2+ ion per subunit.SAAS annotation

GO - Molecular functioni

  1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. nucleic acid binding Source: InterPro

GO - Biological processi

  1. DNA recombination Source: UniProtKB-KW
  2. DNA repair Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, NucleaseSAAS annotation

Keywords - Biological processi

DNA damage, DNA recombinationSAAS annotation, DNA repairSAAS annotation

Keywords - Ligandi

MagnesiumSAAS annotation, Metal-bindingSAAS annotation

Enzyme and pathway databases

BioCyciBWEI315730:GHRU-5855-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCSAAS annotation (EC:3.1.22.4SAAS annotation)
Gene namesi
Ordered Locus Names:BcerKBAB4_5300Imported
Encoded oniPlasmid pBWB403Imported
OrganismiBacillus weihenstephanensis (strain KBAB4)Imported
Taxonomic identifieri315730 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000002154 Componenti: Plasmid pBWB403

Interactioni

Protein-protein interaction databases

STRINGi315730.BcerKBAB4_5300.

Family & Domainsi

Phylogenomic databases

eggNOGiCOG0817.
KOiK01159.
OMAiYLIRENL.
OrthoDBiEOG6RG044.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR012337. RNaseH-like_dom.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.

Sequencei

Sequence statusi: Complete.

A9VVH9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSRRFLGLD MSLASAGIAI IDIVDGELRI VHKQRIKTNP KQRHGERLHK
60 70 80 90 100
IATELKSILI EYQPFDTIIR EQGFSRFAKT TQALFKVVGV SDLVLRDYAI
110 120 130 140 150
VEISPTTIKL VMTGDGKADK KEVEKAVRKI MRLDDDYIFI SDDESDACAI
160
ILTHLIQNNI IDKGDK
Length:166
Mass (Da):18,769
Last modified:February 5, 2008 - v1
Checksum:i41E6142CB6C6AE0D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000906 Genomic DNA. Translation: ABY46794.1.
RefSeqiYP_001642769.1. NC_010182.1.

Genome annotation databases

EnsemblBacteriaiABY46794; ABY46794; BcerKBAB4_5300.
KEGGibwe:BcerKBAB4_5300.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000906 Genomic DNA. Translation: ABY46794.1.
RefSeqiYP_001642769.1. NC_010182.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi315730.BcerKBAB4_5300.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABY46794; ABY46794; BcerKBAB4_5300.
KEGGibwe:BcerKBAB4_5300.

Phylogenomic databases

eggNOGiCOG0817.
KOiK01159.
OMAiYLIRENL.
OrthoDBiEOG6RG044.

Enzyme and pathway databases

BioCyciBWEI315730:GHRU-5855-MONOMER.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR012337. RNaseH-like_dom.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiA9VVH9_BACWK
AccessioniPrimary (citable) accession number: A9VVH9
Entry historyi
Integrated into UniProtKB/TrEMBL: February 5, 2008
Last sequence update: February 5, 2008
Last modified: April 1, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported, PlasmidImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.