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A9VU74 (MURD_BACWK) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9
Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
Gene names
Name:murD
Ordered Locus Names:BcerKBAB4_3739
OrganismBacillus weihenstephanensis (strain KBAB4) [Complete proteome] [HAMAP]
Taxonomic identifier315730 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length450 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639

Catalytic activity

ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639

Subcellular location

Cytoplasm By similarity HAMAP MF_00639.

Sequence similarities

Belongs to the MurCDEF family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 450450UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639
PRO_1000130828

Regions

Nucleotide binding119 – 1257ATP Potential

Sequences

Sequence LengthMass (Da)Tools
A9VU74 [UniParc].

Last modified February 5, 2008. Version 1.
Checksum: 3B161B4B2845D175

FASTA45048,645
        10         20         30         40         50         60 
MKTVTEYQNK NVLVLGIAKS GYAAATLLKK LGANVIVNDG KPLAGNVLAA ELQAEGMDVV 

        70         80         90        100        110        120 
CGGHPLELLE RNISLVVKNP GIPYSNPLLV AAKEKQIPIV TEVELAYSIS AAPFVGITGS 

       130        140        150        160        170        180 
NGKTTTTMLT FEMLKEGQKH PVIAGNIGTV ACEVAQLAKE NEVVVTELSS FQLMGVESFQ 

       190        200        210        220        230        240 
PKIAAFLNLF EAHLDYHGTK KEYGLAKANI FKNQTETDYS IINADDADVM ALSAYSKGQK 

       250        260        270        280        290        300 
VLFSTTKEIE DGAYIKDNAL YFKGEKVVKV SDIVLPGKHN LENILAAMSI AKLLGVSNEA 

       310        320        330        340        350        360 
ITAVLKSFTG VKHRLEYVTT INNRKFYNDS KATNMLATEK ALSAFTQPIV LLAGGLDRGN 

       370        380        390        400        410        420 
EFDDLIPYFK HVKAIVTFGQ TAPKLVRAAE KAGLDVIESV DTLDDAVVKA YAHSTDGDVI 

       430        440        450 
LLSPACASWD QFKTFEERGD IFIQAVHKLI

« Hide

References

[1]"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity."
Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A.
Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KBAB4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000903 Genomic DNA. Translation: ABY44908.1.
RefSeqYP_001646536.1. NC_010184.1.

3D structure databases

ProteinModelPortalA9VU74.
ModBaseSearch...

Protein-protein interaction databases

STRINGA9VU74.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000077374; EBBACP00000075308; EBBACG00000077365.
GeneID5843951.
GenomeReviewsGene locus BcerKBAB4_3739 in contig CP000903_GR.
KEGGbwe:BcerKBAB4_3739.
PATRIC19012113. VBIBacWei55973_4373.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00070000032130.
HOGENOMHBG750024.
OMAACASWDM.
ProtClustDBPRK02472.

Enzyme and pathway databases

BioCycBWEI315730:BCERKBAB4_3739-MONOMER.

Family and domain databases

HAMAPMF_00639. MurD.
[Tree]
InterProIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR016040. NAD(P)-bd_dom.
IPR005762. UDP-N-AcMur-Glu_ligase.
[Graphical view]
Gene3DG3DSA:3.90.190.20. Mur_ligase_C. 1 hit.
G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK01925.
PANTHERPTHR23135:SF2. PTHR23135:SF2. 1 hit.
PfamPF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMSSF53244. Mur_ligase_C. 1 hit.
SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMsTIGR01087. MurD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMURD_BACWK
AccessionPrimary (citable) accession number: A9VU74
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 5, 2008
Last modified: January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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