Reviewed, UniProtKB/Swiss-Prot A9VTC2 (PYRE_BACWK)

Names and origin

Protein names

Recommended name:
    Orotate phosphoribosyltransferase
      Short name=OPRT
      Short name=OPRTase
    EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	BcerKBAB4_3709

Organism

Bacillus weihenstephanensis (strain KBAB4) [Complete proteome] [HAMAP]

Taxonomic identifier

315730 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 210	210	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_1000138763
Regions
	Region	120 – 128	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	94	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	98	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	100	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	124	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9VTC2-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 1D27FF4A35274355	FASTA	210	22,689
	10 20 30 40 50 60 MKKEIASHLL EIGAVFLQPN DPFTWSSGIK SPIYCDNRLT LSYPKVRQAI AAGLEELIKE 70 80 90 100 110 120 HFSTVEVIAG TATAGIAHAA WVSDRMDLPM CYVRSKAKGH GKGNQIEGKA EKGQKVVVVE 130 140 150 160 170 180 DLISTGGSAI TCVEALREAG CEVLGIVSIF TYELESGKEK LEAANVASYS LSDYSALTEV 190 200 210 AAEKGMIGQA ETKKLQEWRK NPANEAWITA

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000903 Genomic DNA. Translation: ABY44878.1.
RefSeq	YP_001646506.1.
3D structure databases
SMR	A9VTC2. Positions 1-204.
ModBase	Search...
Genome annotation databases
GeneID	5843921.
GenomeReviews	Gene locus BcerKBAB4_3709 in contig CP000903_GR.
KEGG	bwe:BcerKBAB4_3709.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG404341.
OMA	PMCYVRS.
Family and domain databases
HAMAP	MF_01208_B. PyrE_B. [Tree]
InterPro	IPR004467. Or_phspho_trans. IPR000836. PRibTrfase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. pyrE. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_BACWK

Accession

Primary (citable) accession number: A9VTC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 5, 2008
Last modified:	February 9, 2010
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)