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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Bacillus weihenstephanensis (strain KBAB4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 156FADUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciBWEI315730:GHRU-3770-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Ordered Locus Names:BcerKBAB4_3655
OrganismiBacillus weihenstephanensis (strain KBAB4)
Taxonomic identifieri315730 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000002154 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 434434Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOPRO_0000346321Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi315730.BcerKBAB4_3655.

Structurei

3D structure databases

ProteinModelPortaliA9VT70.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiEOG6J74VT.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
HAMAPiMF_01037. TrmFO.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9VT70-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTQVVNVIG AGLAGSEAAY QIAKRGVQVK LYEMRPVRQT PAHHTDKFAE
60 70 80 90 100
LVCSNSLRAN TLTNAVGVIK EEMRLMDSVI IRAADECSVP AGGALAVDRH
110 120 130 140 150
EFAAKVTEYV KNHPNVTVMN EEITEIPEGP TVIATGPLTS PDLSAQLKKL
160 170 180 190 200
TGEDYFYFYD AAAPIVEKDS IDMNKVYLKS RYDKGEAAYL NCPMTEEEFD
210 220 230 240 250
RFYEALIAAE TVPLKEFEKE IFFEGCMPVE VMASRGRQTL VFGPMKPVGL
260 270 280 290 300
EDPKTGKTPY AVVQLRQDDA AGTLYNIVGF QTHLKWGPQK EVLQLIPGLE
310 320 330 340 350
NAEIVRYGVM HRNTFINSPN LLRPTYQYKQ RDDLFFAGQM TGVEGYVESA
360 370 380 390 400
ASGLLAGINA ARLVKGEEPV VLPPVTAMGS MANYITATNA KNFQPMNANF
410 420 430
GLFTPLEKKI KKKQERNEAY ATRALETIQN FVNI
Length:434
Mass (Da):48,054
Last modified:February 5, 2008 - v1
Checksum:i7F1F43650F6D8CF8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000903 Genomic DNA. Translation: ABY44826.1.
RefSeqiWP_002014534.1. NC_010184.1.

Genome annotation databases

EnsemblBacteriaiABY44826; ABY44826; BcerKBAB4_3655.
GeneIDi22937936.
KEGGibwe:BcerKBAB4_3655.
PATRICi19011947. VBIBacWei55973_4290.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000903 Genomic DNA. Translation: ABY44826.1.
RefSeqiWP_002014534.1. NC_010184.1.

3D structure databases

ProteinModelPortaliA9VT70.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi315730.BcerKBAB4_3655.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABY44826; ABY44826; BcerKBAB4_3655.
GeneIDi22937936.
KEGGibwe:BcerKBAB4_3655.
PATRICi19011947. VBIBacWei55973_4290.

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiEOG6J74VT.

Enzyme and pathway databases

BioCyciBWEI315730:GHRU-3770-MONOMER.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
HAMAPiMF_01037. TrmFO.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KBAB4.

Entry informationi

Entry nameiTRMFO_BACWK
AccessioniPrimary (citable) accession number: A9VT70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: February 5, 2008
Last modified: January 20, 2016
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.