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A9VT70 (TRMFO_BACWK) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO
EC=2.1.1.74
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferase
Folate-dependent tRNA(M-5-U54)-methyltransferase
Gene names
Name:trmFO
Ordered Locus Names:BcerKBAB4_3655
OrganismBacillus weihenstephanensis (strain KBAB4) [Complete proteome] [HAMAP]
Taxonomic identifier315730 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group ›

Protein attributes

Sequence length434 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity. HAMAP-Rule MF_01037

Catalytic activity

5,10-methylenetetrahydrofolate + uridine54 in tRNA + FADH2 = tetrahydrofolate + 5-methyluridine54 in tRNA + FAD. HAMAP-Rule MF_01037

Cofactor

FAD By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the MnmG family. TrmFO subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 434434Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO HAMAP-Rule MF_01037
PRO_0000346321

Regions

Nucleotide binding10 – 156FAD By similarity

Sequences

Sequence LengthMass (Da)Tools
A9VT70 [UniParc].

Last modified February 5, 2008. Version 1.
Checksum: 7F1F43650F6D8CF8

FASTA43448,054
        10         20         30         40         50         60 
MTTQVVNVIG AGLAGSEAAY QIAKRGVQVK LYEMRPVRQT PAHHTDKFAE LVCSNSLRAN 

        70         80         90        100        110        120 
TLTNAVGVIK EEMRLMDSVI IRAADECSVP AGGALAVDRH EFAAKVTEYV KNHPNVTVMN 

       130        140        150        160        170        180 
EEITEIPEGP TVIATGPLTS PDLSAQLKKL TGEDYFYFYD AAAPIVEKDS IDMNKVYLKS 

       190        200        210        220        230        240 
RYDKGEAAYL NCPMTEEEFD RFYEALIAAE TVPLKEFEKE IFFEGCMPVE VMASRGRQTL 

       250        260        270        280        290        300 
VFGPMKPVGL EDPKTGKTPY AVVQLRQDDA AGTLYNIVGF QTHLKWGPQK EVLQLIPGLE 

       310        320        330        340        350        360 
NAEIVRYGVM HRNTFINSPN LLRPTYQYKQ RDDLFFAGQM TGVEGYVESA ASGLLAGINA 

       370        380        390        400        410        420 
ARLVKGEEPV VLPPVTAMGS MANYITATNA KNFQPMNANF GLFTPLEKKI KKKQERNEAY 

       430 
ATRALETIQN FVNI

« Hide

References

[1]"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity."
Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A.
Chem. Biol. Interact. 171:236-249(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KBAB4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000903 Genomic DNA. Translation: ABY44826.1.
RefSeqYP_001646454.1. NC_010184.1.

3D structure databases

ProteinModelPortalA9VT70.
ModBaseSearch...

Protein-protein interaction databases

STRING315730.BcerKBAB4_3655.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABY44826; ABY44826; BcerKBAB4_3655.
GeneID5843867.
KEGGbwe:BcerKBAB4_3655.
PATRIC19011947. VBIBacWei55973_4290.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1206.
HOGENOMHOG000252054.
KOK04094.
OMARFAGQIT.
ProtClustDBPRK05335.

Enzyme and pathway databases

BioCycBWEI315730:GHRU-3770-MONOMER.

Family and domain databases

HAMAPMF_01037. TrmFO.
InterProIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsTIGR00137. gid_trmFO. 1 hit.
PROSITEPS01280. GIDA_1. False negative.
PS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMFO_BACWK
AccessionPrimary (citable) accession number: A9VT70
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: February 5, 2008
Last modified: May 1, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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