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Protein

Aspartate--tRNA ligase

Gene

aspS

Organism
Bacillus weihenstephanensis (strain KBAB4)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp).UniRule annotation

Catalytic activityi

ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei165AspartateUniRule annotation1
Binding sitei209AspartateUniRule annotation1
Binding sitei355ATPUniRule annotation1
Binding sitei358AspartateUniRule annotation1
Binding sitei362AspartateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi209 – 211ATPUniRule annotation3
Nucleotide bindingi217 – 219ATPUniRule annotation3
Nucleotide bindingi403 – 406ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetaseUniRule annotationImported, Ligase
Biological processProtein biosynthesisUniRule annotation
LigandATP-bindingUniRule annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA ligaseUniRule annotation (EC:6.1.1.12UniRule annotation)
Alternative name(s):
Aspartyl-tRNA synthetaseUniRule annotation
Short name:
AspRSUniRule annotation
Gene namesi
Name:aspSUniRule annotation
Ordered Locus Names:BcerKBAB4_2021Imported
OrganismiBacillus weihenstephanensis (strain KBAB4)Imported
Taxonomic identifieri315730 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000002154 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi315730.BcerKBAB4_2021.

Structurei

3D structure databases

ProteinModelPortaliA9VT09.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini132 – 432AA_TRNA_LIGASE_IIInterPro annotationAdd BLAST301

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni187 – 190AspartateUniRule annotation4

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C5S. Bacteria.
COG0017. LUCA.
HOGENOMiHOG000226032.
KOiK09759.
OMAiPFYAMDD.

Family and domain databases

HAMAPiMF_02075. Asp_tRNA_synth_type2. 1 hit.
InterProiView protein in InterPro
IPR004364. aa-tRNA-synt_II.
IPR006195. aa-tRNA-synth_II.
IPR004523. Asp-tRNA_synthase_2.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
PfamiView protein in Pfam
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00458. aspS_nondisc. 1 hit.
PROSITEiView protein in PROSITE
PS50862. AA_TRNA_LIGASE_II. 1 hit.

Sequencei

Sequence statusi: Complete.

A9VT09-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQIMKRSLT RECTEHGGKV VLLQGWVKKI RHLGNVSFLL LRDRTGVIQC
60 70 80 90 100
VLENELAGYK VDVESVVQVV GEIVETSKTE LGVEVLAHEV KVLNGAEPLP
110 120 130 140 150
FEINKKKLQV GLDQLLNERV LSLRHERTAA IFKVKSTLVQ SFGEFLIEND
160 170 180 190 200
FTRVFTPKIV SQGAEGGANV FKLPYFQKEA YLAQSPQFYK QMMVAGGLER
210 220 230 240 250
VFEIAPVYRA EHHNSSRHLN EYISLDVELG FINDFYEVMQ LETDVLRYMF
260 270 280 290 300
QQVGETCEKE LQLLQIEVPV ITEIPKITLL EAQDILKNKY RKESQIGDLD
310 320 330 340 350
TEGEKLLGKY VKETYNSEFV FITHYPKESR PMYTMPNKEN EAITDSFDLL
360 370 380 390 400
YKGLEITSGA QRIHNHEMLL ASFKEKGLHP DKFQSYVNTF RYGCPPHGGF
410 420 430
GIGLERVVYK LLELSNVREA SAFPRDCTRL IP
Length:432
Mass (Da):49,432
Last modified:February 5, 2008 - v1
Checksum:i5CC85638F7D87990
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000903 Genomic DNA. Translation: ABY43248.1.
RefSeqiWP_012260831.1. NC_010184.1.

Genome annotation databases

EnsemblBacteriaiABY43248; ABY43248; BcerKBAB4_2021.
KEGGibwe:BcerKBAB4_2021.

Similar proteinsi

Entry informationi

Entry nameiA9VT09_BACWK
AccessioniPrimary (citable) accession number: A9VT09
Entry historyiIntegrated into UniProtKB/TrEMBL: February 5, 2008
Last sequence update: February 5, 2008
Last modified: September 27, 2017
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteomeImported