A9VS98 (NUOA_BACWK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit A
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit A
NDH-1 subunit A
NUO1

Gene names

Name:	nuoA
Ordered Locus Names:	BcerKBAB4_5098

Organism

Bacillus weihenstephanensis (strain KBAB4) [Complete proteome] [HAMAP]

Taxonomic identifier

315730 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	122 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP MF_01394
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01394
Subunit structure	NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP MF_01394.
Sequence similarities	Belongs to the complex I subunit 3 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 122	122	NADH-quinone oxidoreductase subunit A HAMAP MF_01394		PRO_0000362625
Regions
	Transmembrane	10 – 30	21	Helical; Potential
	Transmembrane	66 – 86	21	Helical; Potential
	Transmembrane	91 – 111	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	A9VS98 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: E23322808B1F1AD4	FASTA	122	14,010
	10 20 30 40 50 60 MASVYENSYM IVLIFLLLGI LLPVVALTLG KMLRPNKPSA AKATTYESGI EPFHDANIRF 70 80 90 100 110 120 HARYYIFALL FVIFDVETLF LYPWAVAYDK LGLFALIEML IFVVMLLVGL AYAWKKKVLQ WL

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KBAB4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000903 Genomic DNA. Translation: ABY46244.1.
RefSeq	YP_001647872.1. NC_010184.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A9VS98.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000081632; EBBACP00000079566; EBBACG00000081623.
GeneID	5845339.
GenomeReviews	Gene locus BcerKBAB4_5098 in contig CP000903_GR.
KEGG	bwe:BcerKBAB4_5098.
PATRIC	19014949. VBIBacWei55973_5761.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000003148.
HOGENOM	HBG605540.
OMA	SDWIAIG.
ProtClustDB	PRK07756.
Enzyme and pathway databases
BioCyc	BWEI315730:BCERKBAB4_5098-MONOMER.
Family and domain databases
HAMAP	MF_01394. NDH1_NuoA. [Tree]
InterPro	IPR023043. NAD(P)H_OxRDtase_bac/plastid. IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3. [Graphical view]
KO	K00330.
PANTHER	PTHR11058. Oxidored_q4. 1 hit.
Pfam	PF00507. Oxidored_q4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOA_BACWK

Accession

Primary (citable) accession number: A9VS98

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program