A9VRT5 (NAMA_BACWK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH dehydrogenase
EC=1.6.99.1

Gene names

Name:	namA
Ordered Locus Names:	BcerKBAB4_1888

Organism

Bacillus weihenstephanensis (strain KBAB4) [Complete proteome] [HAMAP]

Taxonomic identifier

315730 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group › Bacillus weihenstephanensis

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reduction of the double bond of an array of alpha,beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes By similarity. HAMAP-Rule MF_01614
Catalytic activity	NADPH + acceptor = NADP+ + reduced acceptor. HAMAP-Rule MF_01614
Cofactor	FMN By similarity. HAMAP-Rule MF_01614
Subunit structure	Homotetramer By similarity. HAMAP-Rule MF_01614
Sequence similarities	Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily.

Ontologies

Keywords
Biological process	Detoxification
Ligand	FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	response to toxic substance Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	FMN binding Inferred from electronic annotation. Source: HAMAP NADP binding Inferred from electronic annotation. Source: HAMAP NADPH dehydrogenase activity Inferred from electronic annotation. Source: HAMAP pentaerythritol trinitrate reductase activity Inferred from electronic annotation. Source: EC trichloro-p-hydroquinone reductive dehalogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	NADPH dehydrogenase HAMAP-Rule MF_01614		PRO_1000185864
Regions
	Nucleotide binding	23 – 27	5	FMN By similarity
Sites
	Binding site	28	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	215	1	FMN By similarity
	Binding site	308	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9VRT5 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 79DC0CC8BF22FB43	FASTA	345	38,665
	10 20 30 40 50 60 MEFQLFSPYT IKDVTLKNRI IMSPMCMYSS ENEDGQVTNF HLVHYGTRAA GQVGLVMIEA 70 80 90 100 110 120 TAVLPEGRIS NKDLGIWDDN LIEGLHKTTT FIHNNGAKAA IQLAHAGRKA ELETDAFAPS 130 140 150 160 170 180 AIPFNDRMKI PVEMNKQQIK ETVSAFQKAA LRSKRAGVDV IELHGAHGYL INEFLSPLSN 190 200 210 220 230 240 KRIDEYGGSP ENRYRFLREI IDAVNEVWNG PLFVRISAND YHPDGLTVQD YVQYTGWMKE 250 260 270 280 290 300 QGVDLIDCSS GAVVPAHIDV YPGYQVQYAK HIKEHVNIAT GAVGLITTGS QAEQILNNNE 310 320 330 340 ADLIFIGREL LRNPYFPRIA ANELGFELKE PYQYRRAPGK IKTNK

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KBAB4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000903 Genomic DNA. Translation: ABY43120.1.
RefSeq	YP_001644748.1. NC_010184.1.
3D structure databases
ProteinModelPortal	A9VRT5.
SMR	A9VRT5. Positions 4-337.
ModBase	Search...
Protein-protein interaction databases
STRING	315730.BcerKBAB4_1888.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABY43120; ABY43120; BcerKBAB4_1888.
GeneID	5842101.
KEGG	bwe:BcerKBAB4_1888.
PATRIC	19008345. VBIBacWei55973_2491.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1902.
HOGENOM	HOG000116232.
KO	K00356.
OMA	EPYTLRQ.
ProtClustDB	PRK13523.
Enzyme and pathway databases
BioCyc	BWEI315730:GHRU-2004-MONOMER.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01614. NamA.
InterPro	IPR013785. Aldolase_TIM. IPR023663. NADPH_DH. IPR001155. OxRdtase_FMN_N. [Graphical view]
Pfam	PF00724. Oxidored_FMN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAMA_BACWK

Accession

Primary (citable) accession number: A9VRT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	February 5, 2008
Last modified:	May 29, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program