Reviewed, UniProtKB/Swiss-Prot A9VRQ8 (NADE_BACWK)

Names and origin

Protein names

Recommended name:
    NH(3)-dependent NAD(+) synthetase
    EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	BcerKBAB4_1861

Organism

Bacillus weihenstephanensis (strain KBAB4) [Complete proteome] [HAMAP]

Taxonomic identifier

315730 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000099000
Regions
	Nucleotide binding	45 – 52	8	ATP By similarity
Sites
	Active site	47	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9VRQ8-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 6172F21E33F3D4D7	FASTA	272	30,131
	10 20 30 40 50 60 MTLQEQIMKA LHVQPVIDPK VEIRKRIDFL KDYLKTTGAK GFVLGISGGQ DSTLAGRLAQ 70 80 90 100 110 120 LAVAEVRNEG GNATFISVRL PYKVQKDEDD AQLALQFIQA DQSVAFDIAS TVDAFSNQYE 130 140 150 160 170 180 NLLDESLTDF NKGNVKARIR MVTQYAIGGQ QGLLVIGTDH AAEAVTGFFT KFGDGGADLL 190 200 210 220 230 240 PLTGLTKRQG RDLLQELGAD ERLYLKMPTA DLLDEKPGQA DETELGITYD QLDDYLEGKS 250 260 270 VPADVAEKIE KRYKVSEHKR QVPASMFDDW WK

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000903 Genomic DNA. Translation: ABY43093.1.
RefSeq	YP_001644721.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5842074.
GenomeReviews	Gene locus BcerKBAB4_1861 in contig CP000903_GR.
KEGG	bwe:BcerKBAB4_1861.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	ADLEEDR.
Family and domain databases
HAMAP	MF_00193. [Tree]
InterPro	IPR003694. NAD_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. nadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_BACWK

Accession

Primary (citable) accession number: A9VRQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 5, 2008
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)