Reviewed, UniProtKB/Swiss-Prot A9VPX5 (LEXA_BACWK)

Names and origin

Protein names

Recommended name:
    LexA repressor
    EC=3.4.21.88

Gene names

Name:	lexA
Ordered Locus Names:	BcerKBAB4_3377

Organism

Bacillus weihenstephanensis (strain KBAB4) [Complete proteome] [HAMAP]

Taxonomic identifier

315730 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity.
Catalytic activity	Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the peptidase S24 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Hydrolase Repressor
PTM	Autocatalytic cleavage
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP proteolysis Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 204	204	LexA repressor HAMAP MF_00015		PRO_1000089545
Regions
	DNA binding	28 – 48	21	H-T-H motif By similarity
Sites
	Active site	126	1	For autocatalytic cleavage activity By similarity
	Active site	164	1	For autocatalytic cleavage activity By similarity
	Site	90 – 91	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9VPX5-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 5C98C94FBEE03BA1	FASTA	204	22,629
	10 20 30 40 50 60 MEKLTKRQQD ILDFIKLKVQ EKGYPPSVRE IGQAVGLASS STVHGHLSRL EEKGYIRRDP 70 80 90 100 110 120 TKPRAIEILG DNRTETQSVI QVPIIGKVTA GLPITAVESV EDHFPLPASI VAGADQVFML 130 140 150 160 170 180 RISGDSMIEA GIFDGDLVVV RQQQSAYNGE IVVALTEDNE ATVKRFYKEK DHFRLQPENS 190 200 SLEPIILKQV SVIGKVIGVY RDLH

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000903 Genomic DNA. Translation: ABY44551.1.
RefSeq	YP_001646179.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	S24.001.
Genome annotation databases
GeneID	5843589.
GenomeReviews	Gene locus BcerKBAB4_3377 in contig CP000903_GR.
KEGG	bwe:BcerKBAB4_3377.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	KVIGVFR.
Family and domain databases
HAMAP	MF_00015. [Tree]
InterPro	IPR006199. LexA_DNA_bd. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA_cons-reg. IPR019759. Peptidase_S24_S26_cons-reg. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00726. LEXASERPTASE.
TIGRFAMs	TIGR00498. lexA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEXA_BACWK

Accession

Primary (citable) accession number: A9VPX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 5, 2008
Last modified:	November 3, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)