A9VPK1 (ASNA_BACWK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase
EC=6.3.1.1

Alternative name(s):
Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	BcerKBAB4_1672

Organism

Bacillus weihenstephanensis (strain KBAB4) [Complete proteome] [HAMAP]

Taxonomic identifier

315730 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000129110

Sequences

	Sequence		Length	Mass (Da)
	A9VPK1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: C31776804598D180	FASTA	327	38,084
	10 20 30 40 50 60 MYQSLMTVRE TQIAIKEVKT FFEDQLAKRL ELFRVSAPLF VTKKSGLNDH LNGVERPIEF 70 80 90 100 110 120 DMLHSGEELE IVHSLAKWKR FALHEYGYEA GEGLYTNMNA IRRDEELDAT HSIYVDQWDW 130 140 150 160 170 180 EKIVQKEWRT IDYLQKTVQT IYGIFKELEG HLFEKYPFLG KYLPEEIIFI TSQELEDKYP 190 200 210 220 230 240 ELTPKDREHA IAKEHGAVFI IGIGDALRSG EKHDGRASDY DDWKLNGDIL FWHPVLQASF 250 260 270 280 290 300 ELSSMGIRVD SKALDEQLTK TGEDFKREYD FHKGILEDVL PLTIGGGIGQ SRMCMYFLRK 310 320 AHIGEVQSSV WPDDLREACK KENIHLF

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KBAB4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000903 Genomic DNA. Translation: ABY42913.1.
RefSeq	YP_001644541.1. NC_010184.1.
3D structure databases
ProteinModelPortal	A9VPK1.
ModBase	Search...
Protein-protein interaction databases
STRING	A9VPK1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000079205; EBBACP00000077139; EBBACG00000079196.
GeneID	5841885.
GenomeReviews	Gene locus BcerKBAB4_1672 in contig CP000903_GR.
KEGG	bwe:BcerKBAB4_1672.
PATRIC	19007907. VBIBacWei55973_2272.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000003597.
HOGENOM	HBG288146.
OMA	LNDNLNG.
ProtClustDB	PRK05425.
Enzyme and pathway databases
BioCyc	BWEI315730:BCERKBAB4_1672-MONOMER.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_BACWK

Accession

Primary (citable) accession number: A9VPK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program