Reviewed, UniProtKB/Swiss-Prot A9VKS1 (QUEF_BACWK)

Names and origin

Protein names

Recommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductase
    EC=1.7.1.13
Alternative name(s):
    7-cyano-7-carbaguanine reductase
    PreQ(0) reductase
    NADPH-dependent nitrile oxidoreductase

Gene names

Name:	queF
Ordered Locus Names:	BcerKBAB4_1259

Organism

Bacillus weihenstephanensis (strain KBAB4) [Complete proteome] [HAMAP]

Taxonomic identifier

315730 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	165 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity.
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00818
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00818
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP queuine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 165	165	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00818		PRO_1000134296

Sequences

	Sequence		Length	Mass (Da)
	A9VKS1-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: E08FBCDAB28FFAE3	FASTA	165	19,555
	10 20 30 40 50 60 MAGRLDEDLK DVTLLGNQNT KYLFEYSPEI LEVFDNNHPN RDYFVKFNCP EFTSLCPKTG 70 80 90 100 110 120 QPDFATIYIS YIPEQRMVES KSLKLYLFSF RNHGDFHEDC MNVIMNDLIK LMDPRYIEVW 130 140 150 160 GKFTPRGGIS IDPYCNYGRP GTKYEKMADY RMMNHDLYPE KIDNR

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000903 Genomic DNA. Translation: ABY42506.1.
RefSeq	YP_001644134.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5841473.
GenomeReviews	Gene locus BcerKBAB4_1259 in contig CP000903_GR.
KEGG	bwe:BcerKBAB4_1259.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	HESCINT.
Family and domain databases
HAMAP	MF_00818. [Tree]
InterPro	IPR016856. CN_OxRdtase_NADPH-dep_QueF. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMs	TIGR03139. QueF-II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_BACWK

Accession

Primary (citable) accession number: A9VKS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 5, 2008
Last modified:	November 3, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)