A9VK31 (PROA_BACWK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	BcerKBAB4_2789

Organism

Bacillus weihenstephanensis (strain KBAB4) [Complete proteome] [HAMAP]

Taxonomic identifier

315730 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate By similarity. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_1000193571

Sequences

	Sequence		Length	Mass (Da)
	A9VK31 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 0F1DA130039F4277	FASTA	415	45,556
	10 20 30 40 50 60 MNEVVAKGKK AKEIARELVL KSTNQKNEAL AAIADQMIVE TAYILEENKR DIEEGKAKGF 70 80 90 100 110 120 SDSLLDRLML NEQRIIDMAE GIKQLIELRD PVGECVSAWE RPNGLSIQEM RVPLGVVGMI 130 140 150 160 170 180 YEARPNVTVD AATICLKTGN AVILRGSSSA INSNKAIVSV IHRALKQTSL PPESVQLIED 190 200 210 220 230 240 TTRDSAKQLF TLNEYLDVLI PRGGKQLIDT VVREASVPVL ETGAGNCHIF IDETADKQMA 250 260 270 280 290 300 FNIIINAKTQ RPSVCNAIET IVLHEKWAQQ FGSELFSSLK ERGVELRGDS KSLAIDSSIA 310 320 330 340 350 360 LASEEDWETE FLSLTLAVKV VSTTEEAIHH INTYGSMHSE AIITENEENV SKFFTSVDAA 370 380 390 400 410 ALYHNASTRF TDGSEFGFGA EIGISTQKLH VRGPMGLPAL TSTKYVIRGN GQIRR

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KBAB4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000903 Genomic DNA. Translation: ABY43986.1.
RefSeq	YP_001645614.1. NC_010184.1.
3D structure databases
ProteinModelPortal	A9VK31.
SMR	A9VK31. Positions 3-415.
ModBase	Search...
Protein-protein interaction databases
STRING	A9VK31.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000079313; EBBACP00000077247; EBBACG00000079304.
GeneID	5843001.
GenomeReviews	Gene locus BcerKBAB4_2789 in contig CP000903_GR.
KEGG	bwe:BcerKBAB4_2789.
PATRIC	19010189. VBIBacWei55973_3411.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000000020.
HOGENOM	HBG318080.
OMA	QYPAACN.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	BWEI315730:BCERKBAB4_2789-MONOMER.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
KO	K00147.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_BACWK

Accession

Primary (citable) accession number: A9VK31

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program