A9VIV4 (RNPH_BACWK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56

Alternative name(s):
tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	BcerKBAB4_4325

Organism

Bacillus weihenstephanensis (strain KBAB4) [Complete proteome] [HAMAP]

Taxonomic identifier

315730 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	tRNA binding Inferred from electronic annotation. Source: InterPro tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: EC tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Ribonuclease PH HAMAP MF_00564		PRO_1000129322

Sequences

	Sequence		Length	Mass (Da)
	A9VIV4 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 8E21C66EB4211CD1	FASTA	245	26,899
	10 20 30 40 50 60 MRVDGRGKAE LRHIHIHTNY LKHPEGSVLI EVGDTKVICS ATIEERVPPF MRGEGKGWVT 70 80 90 100 110 120 AEYSMIPRAT EQRTIRESSK GKVTGRTMEI QRLIGRALRA VVDLEALGER TVWIDCDVIQ 130 140 150 160 170 180 ADGGTRTASI TGAYVAMVLA FEKLLQAEKV SKIPVKDYLA ATSVGVVEEQ GVVLDLNYAE 190 200 210 220 230 240 DSKADVDMNV IMTGKGQFVE VQGTGEEATF SRAELNELLD AAEQGIFQLV EKQKEALGDI VSHIE

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KBAB4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000903 Genomic DNA. Translation: ABY45484.1.
RefSeq	YP_001647112.1. NC_010184.1.
3D structure databases
ProteinModelPortal	A9VIV4.
SMR	A9VIV4. Positions 1-239.
ModBase	Search...
Protein-protein interaction databases
STRING	A9VIV4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000081369; EBBACP00000079303; EBBACG00000081360.
GeneID	5844540.
GenomeReviews	Gene locus BcerKBAB4_4325 in contig CP000903_GR.
KEGG	bwe:BcerKBAB4_4325.
PATRIC	19013324. VBIBacWei55973_4975.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000001825.
HOGENOM	HBG737187.
OMA	MLPRATG.
ProtClustDB	PRK00173.
Enzyme and pathway databases
BioCyc	BWEI315730:BCERKBAB4_4325-MONOMER.
Family and domain databases
HAMAP	MF_00564. RNase_PH. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
KO	K00989.
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
SUPFAM	SSF55666. 3_ExoRNase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_BACWK

Accession

Primary (citable) accession number: A9VIV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program