Reviewed, UniProtKB/Swiss-Prot A9VIM8 (SYD_BACWK)

Names and origin

Protein names

Recommended name:
    Aspartyl-tRNA synthetase
    EC=6.1.1.12
Alternative name(s):
    Aspartate--tRNA ligase
      Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	BcerKBAB4_4249

Organism

Bacillus weihenstephanensis (strain KBAB4) [Complete proteome] [HAMAP]

Taxonomic identifier

315730 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 591	591	Aspartyl-tRNA synthetase HAMAP MF_00044		PRO_1000090959

Sequences

	Sequence		Length	Mass (Da)
	A9VIM8-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 4EEB174037FC2C33	FASTA	591	66,319
	10 20 30 40 50 60 MAERTHACGK VTVEAVGQTV QLKGWVQKRR DLGGLIFIDL RDRTGIVQVV FNPETSKEAL 70 80 90 100 110 120 EVAETIRSEY VLHVEGTVVE RGEGAINDNM ATGRIEVQAS KVNVLNAAKT TPIIIADDTD 130 140 150 160 170 180 ASEDVRLKYR YLDLRRPAMF NTFKMRHDVT KTIRNFLDTE EFLEVETPIL TKSTPEGARD 190 200 210 220 230 240 YLVPSRVHDG EFYALPQSPQ LFKQLLMVGG FERYYQVARC FRDEDLRADR QPEFTQIDIE 250 260 270 280 290 300 ASFLTQDEIL EMMERMMTKV MKDAKGVEVS APFPRMKYAD AMARFGSDKP DTRFEMELKD 310 320 330 340 350 360 LSEFAVGCGF KVFTSAVENG GQVKAINAKG AASKYSRKDI DALTEFAKVY GAKGLAWLKV 370 380 390 400 410 420 EEDGLKGPIA KFFGEEEANV LMSTLEADAG DLLLFVADKK SVVADSLGAL RLRLGKELEL 430 440 450 460 470 480 IDESKFNFLW VTDWPLLEYD EDADRYFAAH HPFTMPFRED VELLETAPEK ARAQAYDLVL 490 500 510 520 530 540 NGYELGGGSL RIYERDVQEK MFKALGFSQE EAQEQFGFLL EAFEYGTPPH GGIALGLDRL 550 560 570 580 590 VMLLAGRTNL RDTIAFPKTA SASCLLTEAP SPVAEAQLEE LSLKLSLKEE K

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000903 Genomic DNA. Translation: ABY45408.1.
RefSeq	YP_001647036.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5844462.
GenomeReviews	Gene locus BcerKBAB4_4249 in contig CP000903_GR.
KEGG	bwe:BcerKBAB4_4249.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VDRRRDH.
Family and domain databases
HAMAP	MF_00044. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002312. Asp-tRNA-synth_IIb. IPR020564. Asp-tRNA-synth_IIb_bac-type. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR018153. Asp-tRNA-synth_IIb_C_bac/mt. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00459. aspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_BACWK

Accession

Primary (citable) accession number: A9VIM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 5, 2008
Last modified:	November 3, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)