Reviewed, UniProtKB/Swiss-Prot A9VI99 (HEMH_BACWK)

Names and origin

Protein names

Recommended name:
    Ferrochelatase
    EC=4.99.1.1
Alternative name(s):
    Protoheme ferro-lyase
    Heme synthetase

Gene names

Name:	hemH
Ordered Locus Names:	BcerKBAB4_0990

Organism

Bacillus weihenstephanensis (strain KBAB4) [Complete proteome] [HAMAP]

Taxonomic identifier

315730 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX By similarity.
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Ferrochelatase HAMAP MF_00323		PRO_1000116030
Sites
	Metal binding	182	1	Iron By similarity
	Metal binding	263	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9VI99-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 9A1E8A736C368DFD	FASTA	311	35,157
	10 20 30 40 50 60 MKKKIGLLVM AYGTPYKEED IERYYTHIRR GRKPSPEMLE DLTGRYRAIG GISPLATITL 70 80 90 100 110 120 EQAKKLETRL NEMQDEVEYH MYLGLKHIEP FIEDAVQAMH KDGIEDAIAL VLAPHYSTFS 130 140 150 160 170 180 VKSYVGRAQE EAGKLGNLTI HGIDSWYKEP KFIQYWVDAV KGIYNGMSEA EREKAVLIVS 190 200 210 220 230 240 AHSLPEKIIA LGDPYPDQLN ETADYIARGA EVANYAVGWQ SAGNTPDPWI GPDVQDLTRE 250 260 270 280 290 300 LNEKYGYNSF VYAPVGFVAE HLEVLYDNDF ECKVVTDEIG AKYYRPEMPN ASDAFIDCLA 310 DVVLKKKESV M

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000903 Genomic DNA. Translation: ABY42242.1.
RefSeq	YP_001643870.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5841202.
GenomeReviews	Gene locus BcerKBAB4_0990 in contig CP000903_GR.
KEGG	bwe:BcerKBAB4_0990.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	DEDIERY.
Family and domain databases
HAMAP	MF_00323. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
ProDom	PD002792. Ferrochelatase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00109. hemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_BACWK

Accession

Primary (citable) accession number: A9VI99

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 5, 2008
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)