A9VHP7 (T23O_BACWK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan 2,3-dioxygenase
Short name=TDO
EC=1.13.11.11

Alternative name(s):
Tryptamin 2,3-dioxygenase
Tryptophan oxygenase
Short name=TO
Short name=TRPO
Tryptophan pyrrolase
Tryptophanase

Gene names

Name:	kynA
Ordered Locus Names:	BcerKBAB4_2557

Organism

Bacillus weihenstephanensis (strain KBAB4) [Complete proteome] [HAMAP]

Taxonomic identifier

315730 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	Tryptophan 2,3-dioxygenase		PRO_0000360088
Regions
	Region	23 – 27	5	Substrate binding By similarity
	Region	48 – 52	5	Substrate binding By similarity
Sites
	Metal binding	237	1	Iron (heme axial ligand) By similarity
	Binding site	110	1	Substrate By similarity
	Binding site	114	1	Substrate By similarity
	Binding site	121	1	Heme By similarity
	Binding site	251	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9VHP7 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: B1614633979B67CA	FASTA	279	32,733
	10 20 30 40 50 60 MKENEKVIME KGIHTDFKEN MTYGEYLQLD SLLSSQQRLS DHHDEMLFIV IHQASELWMK 70 80 90 100 110 120 LILHELNAAI ESIKQDKLPP AFKMLARVSK IQSQIIQSWD ILATLTPSEY IEFRDSLGQA 130 140 150 160 170 180 SGFQSYQYRM IEYALGYKTP HALKIYEKDP ELHARLHKAL HTPSLYDVAI QALVKEGFPI 190 200 210 220 230 240 HKDVLNRDIT QPYEEDATVE AAWIEVYADV QKYWDLYQLA EKLIDIEDWL QQWRFRHMKT 250 260 270 VERIIGHKMG TGGSSGVSYL KRVLDQCFFP ELWNVRTKL

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KBAB4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000903 Genomic DNA. Translation: ABY43761.1.
RefSeq	YP_001645389.1. NC_010184.1.
3D structure databases
ProteinModelPortal	A9VHP7.
SMR	A9VHP7. Positions 20-279.
ModBase	Search...
Protein-protein interaction databases
STRING	A9VHP7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000080295; EBBACP00000078229; EBBACG00000080286.
GeneID	5842770.
GenomeReviews	Gene locus BcerKBAB4_2557 in contig CP000903_GR.
KEGG	bwe:BcerKBAB4_2557.
PATRIC	19009719. VBIBacWei55973_3176.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000004238.
HOGENOM	HBG647485.
OMA	KLLVDQV.
ProtClustDB	CLSK904616.
Enzyme and pathway databases
BioCyc	BWEI315730:BCERKBAB4_2557-MONOMER.
Family and domain databases
InterPro	IPR017485. Trp_2-3-dOase_bac. IPR004981. Trp_2_3_dOase. [Graphical view]
KO	K00453.
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
TIGRFAMs	TIGR03036. Trp_2_3_diox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T23O_BACWK

Accession

Primary (citable) accession number: A9VHP7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program