Reviewed, UniProtKB/Swiss-Prot A9VFA6 (METE_BACWK)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	BcerKBAB4_3831

Organism

Bacillus weihenstephanensis (strain KBAB4) [Complete proteome] [HAMAP]

Taxonomic identifier

315730 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	762 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity.
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 762	762	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000097814
Sites
	Metal binding	645	1	Zinc By similarity
	Metal binding	647	1	Zinc By similarity
	Metal binding	730	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9VFA6-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 5FDF92CB1E6B7A2A	FASTA	762	86,849
	10 20 30 40 50 60 MAIQTSNLGY PRIGLQREWK KTLEAFWSNK IDEEQFLTTM KEIRLQHVKV QQEKGIELIP 70 80 90 100 110 120 VGDFTYYDHV LDTAYMLGFI PSRFSEFTSY LDVYFAMARG SKDHVASEMT KWFNTNYHYI 130 140 150 160 170 180 VPEYEEGLQI SLKDNRPLRL YEEAKKELGV DGKPVILGPY TFLKLAKGYN EEQFATILKE 190 200 210 220 230 240 LVAPYVQLFS ELHAAGAQII QVDEPIFASL TKDEINQAKE LYEAIHKEVP NANLLLQTYF 250 260 270 280 290 300 DSVEENYEEI IAFPVSGIGL DFVHGKEGNV NAISKYGFPA DKILAIGCID GRNIWRADLD 310 320 330 340 350 360 DVLSLFTTLQ NQITAKGLIV QPSCSLLHTP IDKTEETHLT SELFDALAFA NQKLEELVLI 370 380 390 400 410 420 HSALTKGVES ISSDLTTYRN AHHAIRSSAA RNREDVKVAR TSLKEDDFSR PLPFEKRYEL 430 440 450 460 470 480 QQVALQLPLL PTTTIGSFPQ TSEVRQTRKQ WRNGDITNEQ YNQFIEKETA KWIRYQEDIG 490 500 510 520 530 540 LDVLVHGEFE RTDMVEYFGE RLAGFSFTKN GWVQSYGSRC VKPPVIYGDV AFISGMTIKE 550 560 570 580 590 600 TVYAQSLTEK VVKGMLTGPV TILNWSFVRN DISRKEVSYQ IALALRHEIE LLESSGIRVI 610 620 630 640 650 660 QVDEPALREG MPLKEKDWDA YITWAVQSFL LATSSVENET QIHTHMCYSN FEDIVDAIRA 670 680 690 700 710 720 LDADVISIET SRSHGEFINT LKHTTYEKGI GLGVYDIHSP RVPSKDEMFT IVEQSLQVCD 730 740 750 760 PKYFWINPDC GLKTRRTEEV IPALEHMVQA AKDARSLLKT NA

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000903 Genomic DNA. Translation: ABY45000.1.
RefSeq	YP_001646628.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5844043.
GenomeReviews	Gene locus BcerKBAB4_3831 in contig CP000903_GR.
KEGG	bwe:BcerKBAB4_3831.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CSLLHTP.
Family and domain databases
HAMAP	MF_00172. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
ProDom	PD004692. Methionine_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BACWK

Accession

Primary (citable) accession number: A9VFA6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 5, 2008
Last modified:	November 3, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)