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Protein

Flap endonuclease 1

Gene

FEN1

Organism
Monosiga brevicollis (Choanoflagellate)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi34Magnesium 1UniRule annotation1
Binding sitei47DNA substrateUniRule annotation1
Binding sitei70DNA substrateUniRule annotation1
Metal bindingi86Magnesium 1UniRule annotation1
Metal bindingi158Magnesium 1UniRule annotation1
Binding sitei158DNA substrateUniRule annotation1
Metal bindingi160Magnesium 1UniRule annotation1
Metal bindingi179Magnesium 2UniRule annotation1
Metal bindingi181Magnesium 2UniRule annotation1
Binding sitei229DNA substrateUniRule annotation1
Metal bindingi231Magnesium 2UniRule annotation1
Binding sitei231DNA substrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Exonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair, DNA replication
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Flap endonuclease 1UniRule annotation (EC:3.1.-.-UniRule annotation)
Short name:
FEN-1UniRule annotation
Alternative name(s):
Flap structure-specific endonuclease 1UniRule annotation
Gene namesi
Name:FEN1UniRule annotation
ORF Names:29430
OrganismiMonosiga brevicollis (Choanoflagellate)
Taxonomic identifieri81824 [NCBI]
Taxonomic lineageiEukaryotaChoanoflagellidaCraspedidaSalpingoecidaeMonosiga
Proteomesi

Subcellular locationi

  • nucleolus UniRule annotation
  • nucleoplasm UniRule annotation
  • Mitochondrion UniRule annotation
  • Note: Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004036091 – 368Flap endonuclease 1Add BLAST368

Post-translational modificationi

Phosphorylated. Phosphorylation upon DNA damage induces relocalization to the nuclear plasma.UniRule annotation

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Interacts with PCNA. Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.UniRule annotation

Protein-protein interaction databases

STRINGi431895.XP_001749886.1

Structurei

3D structure databases

ProteinModelPortaliA9VB27
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 104N-domainAdd BLAST104
Regioni122 – 251I-domainAdd BLAST130
Regioni334 – 342Interaction with PCNAUniRule annotation9

Sequence similaritiesi

Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2519 Eukaryota
COG0258 LUCA
InParanoidiA9VB27
KOiK04799
OMAiGSQDYDS

Family and domain databases

CDDicd09867 PIN_FEN1, 1 hit
HAMAPiMF_00614 Fen, 1 hit
InterProiView protein in InterPro
IPR036279 5-3_exonuclease_C_sf
IPR023426 Flap_endonuc
IPR008918 HhH2
IPR029060 PIN-like_dom_sf
IPR006086 XPG-I_dom
IPR006084 XPG/Rad2
IPR019974 XPG_CS
IPR006085 XPG_DNA_repair_N
PANTHERiPTHR11081 PTHR11081, 1 hit
PfamiView protein in Pfam
PF00867 XPG_I, 1 hit
PF00752 XPG_N, 1 hit
PRINTSiPR00853 XPGRADSUPER
SMARTiView protein in SMART
SM00279 HhH2, 1 hit
SM00484 XPGI, 1 hit
SM00485 XPGN, 1 hit
SUPFAMiSSF47807 SSF47807, 1 hit
SSF88723 SSF88723, 1 hit
PROSITEiView protein in PROSITE
PS00841 XPG_1, 1 hit
PS00842 XPG_2, 1 hit

Sequencei

Sequence statusi: Complete.

A9VB27-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGIHDLSKVI ADKAPDAIKE TEIKNLFDRK VAIDASMSIY QFLIAIRSEG
60 70 80 90 100
SNLVNEAGEA TSHLSGLFYR TIRMVNHGIK PLYVFDGKPP TMKSGELLKR
110 120 130 140 150
GARRKEAQAN LEEATEQGDT EQMEKFSRRL VHVTREHNEQ CRQLLTLMGI
160 170 180 190 200
PFIIAPTEAE AQCAELVKGG KVFATATEDM DALTFGTTVL LRHMTFSEAR
210 220 230 240 250
KMPIQEFRLQ KGGLEMSMEE FIDMCILLGC DYCDSIKGIG RQKAYQLIKE
260 270 280 290 300
HKNIETVLKH LDPKKYVIPE DWHFAEAREL FLRPDVTPAA ECEFKWTTPD
310 320 330 340 350
IDGLVKFMCQ ENGFAEDRIR KSAEKLVKAR KGGQQGRLDS FFTAIPSGSA
360
KRNTVGLLPK PGPPPFLF
Length:368
Mass (Da):41,441
Last modified:February 5, 2008 - v1
Checksum:i233E6980A6B49093
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH991575 Genomic DNA Translation: EDQ85265.1
RefSeqiXP_001749886.1, XM_001749834.1

Genome annotation databases

EnsemblProtistsiEDQ85265; EDQ85265; MONBRDRAFT_29430
GeneIDi5895205
KEGGimbr:MONBRDRAFT_29430

Entry informationi

Entry nameiFEN1_MONBE
AccessioniPrimary (citable) accession number: A9VB27
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: February 5, 2008
Last modified: March 28, 2018
This is version 50 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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