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Protein

Enolase 4

Gene

eno4

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.By similarity
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gapdh), Glyceraldehyde-3-phosphate dehydrogenase (XELAEV_18036725mg), Glyceraldehyde-3-phosphate dehydrogenase (gapdh)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Enolase 4 (eno4), Alpha-enolase (eno1)
  5. Pyruvate kinase, Pyruvate kinase (pklr), Pyruvate kinase PKM (pkm), Pyruvate kinase (LOC100037030)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei288SubstrateBy similarity1
Active sitei468Proton acceptorBy similarity1
Binding sitei519SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase 4 (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
Gene namesi
Name:eno4
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003484571 – 572Enolase 4Add BLAST572

Structurei

3D structure databases

ProteinModelPortaliA9UMP7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

HOVERGENiHBG107850.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 2 hits.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 2 hits.

Sequencei

Sequence statusi: Complete.

A9UMP7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYRVTGDAA RKARERYELK QAAAEFYRKR NVPERLEEAL NSTFCLGPED
60 70 80 90 100
VYGHLANYFA QFSMPPTICQ IRGRKVLDGS GEPTVEVEVS CTVKNSDKRI
110 120 130 140 150
CSSVISAVSE HPKASEGLEQ ERNHSADTAI QWLKDLSPLL KGMSPNEQHN
160 170 180 190 200
IDQLLSDFYQ PKIEEEKARR QMEREASPMA IEPVPSPVTS PALGKKKGSG
210 220 230 240 250
KGKKAVVMEK PIPPKEAPEP VVPGSPAIGA LSLAVAKASS VLGKTPLYLH
260 270 280 290 300
IATLRNEKLP SEFIMPTPMI SVLSCGKSSP GKLNLMKEVL VIPPTGLTVQ
310 320 330 340 350
QSLDMALMLQ NQIVKQINSI SKTGPAIKNV TPLGCMLIGG DRIEQPLDLI
360 370 380 390 400
CEACQHVGLE LGRNLYLAIN CAAHELMDYN KAKYEALSGT FKSPDEMVDL
410 420 430 440 450
YVDLINRQPA ILALLDPLRK EDAAQWESLT KALGSKCFLF ADAASKPVCK
460 470 480 490 500
LLESADIHNP PCSGTVIKHT NETTVSQLLG SFKLIEGENR VTILGCPCEE
510 520 530 540 550
SVDDSIADLA VGLGARFVKL GGLLRGERSS KYNRLLAIED ELTQAGTLGF
560 570
WTKHEFPILS DVENLPGPEE VE
Length:572
Mass (Da):62,419
Last modified:September 2, 2008 - v2
Checksum:i7AD4B020613C70C0
GO

Sequence cautioni

The sequence AAI57742 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC157741 mRNA. Translation: AAI57742.1. Different initiation.
UniGeneiXl.59278.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC157741 mRNA. Translation: AAI57742.1. Different initiation.
UniGeneiXl.59278.

3D structure databases

ProteinModelPortaliA9UMP7.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG107850.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 2 hits.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiENO4_XENLA
AccessioniPrimary (citable) accession number: A9UMP7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: September 2, 2008
Last modified: February 15, 2017
This is version 31 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Although it belongs to the enolase family, Leu-333 is present instead of the conserved Glu which is expected to be an active site residue.Curated

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.