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A9UMM1 (TRMBB_XENTR) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase B

EC=2.1.1.33
Alternative name(s):
Methyltransferase-like protein 1-B
tRNA (guanine(46)-N(7))-methyltransferase B
tRNA(m7G46)-methyltransferase B
Gene names
Name:mettl1-B
OrganismXenopus tropicalis (Western clawed frog) (Silurana tropicalis) [Reference proteome]
Taxonomic identifier8364 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana

Protein attributes

Sequence length241 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subunit structure

Forms a complex with wdr4 By similarity. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 241241tRNA (guanine-N(7)-)-methyltransferase B HAMAP-Rule MF_03055
PRO_0000370561

Regions

Region84 – 852S-adenosyl-L-methionine binding By similarity
Region117 – 1182S-adenosyl-L-methionine binding By similarity
Region215 – 2173S-adenosyl-L-methionine binding By similarity

Sites

Active site1401 By similarity
Binding site611S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1371S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
A9UMM1 [UniParc].

Last modified February 5, 2008. Version 1.
Checksum: 5DC526C10E4F5500

FASTA24127,951
        10         20         30         40         50         60 
MSVCMPQKRY YRQRAHSNPM ADHTFQYPVC PEQMDWSPLY PQYFPQQEEA GGAQVEFADI 

        70         80         90        100        110        120 
GCGYGGLLVQ LSLLFPQQLI LGLEIRVKVS DYVQDRIRSL RVAEPGRYQN IACLRSNAMK 

       130        140        150        160        170        180 
YLPNFFRKGQ LSKMFFLFPD PHFKKTKHKW RIISPTLLAE YAYTLRIGGL VYTNTDVEEV 

       190        200        210        220        230        240 
HEWIVQHFSD HPLFSRVTEE QLADDIIVGH LGTCTEEGKK VQRNGGKNFL AVFRRVEDPQ 


T 

« Hide

References

[1]NIH - Xenopus Gene Collection (XGC) project
Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: TGA IC.
Tissue: Testis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC157713 mRNA. Translation: AAI57714.1.
RefSeqNP_001107533.1. NM_001114061.1.
UniGeneStr.77345.

3D structure databases

ProteinModelPortalA9UMM1.
SMRA9UMM1. Positions 27-239.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100135397.
KEGGxtr:100135397.

Organism-specific databases

CTD100135397.

Phylogenomic databases

KOK03439.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMBB_XENTR
AccessionPrimary (citable) accession number: A9UMM1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: February 5, 2008
Last modified: June 11, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways