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Protein

ATP-dependent 6-phosphofructokinase

Gene

PFK

Organism
Physcomitrella patens subsp. patens (Moss)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by AMP.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (PHYPADRAFT_145746), Glucose-6-phosphate isomerase (PHYPADRAFT_180360), Glucose-6-phosphate isomerase (PHYPADRAFT_115897), Glucose-6-phosphate isomerase (PHYPADRAFT_125345), Glucose-6-phosphate isomerase (PHYPADRAFT_198839)
  3. Probable ATP-dependent 6-phosphofructokinase (PHYPADRAFT_55781), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), Probable ATP-dependent 6-phosphofructokinase (PHYPADRAFT_218994), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK)
  4. Fructose-bisphosphate aldolase (PHYPADRAFT_233547), Fructose-bisphosphate aldolase (PHYPADRAFT_140886), Fructose-bisphosphate aldolase (PHYPADRAFT_120582), Fructose-bisphosphate aldolase (PHYPADRAFT_166170), Fructose-bisphosphate aldolase (PHYPADRAFT_155603), Fructose-bisphosphate aldolase (PHYPADRAFT_148638), Fructose-bisphosphate aldolase (PHYPADRAFT_232361), Fructose-bisphosphate aldolase (PHYPADRAFT_124602), Fructose-bisphosphate aldolase (PHYPADRAFT_56578)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei101ATP; via amide nitrogenUniRule annotation1
Metal bindingi190Magnesium; catalyticUniRule annotation1
Sitei191Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei220Proton acceptorUniRule annotation1
Binding sitei319SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi164 – 165ATPUniRule annotation2
Nucleotide bindingi189 – 192ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:PFKUniRule annotation
ORF Names:PHYPADRAFT_136703Imported
OrganismiPhyscomitrella patens subsp. patens (Moss)Imported
Taxonomic identifieri3218 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaBryophytaBryophytinaBryopsidaFunariidaeFunarialesFunariaceaePhyscomitrella
Proteomesi
  • UP000006727 Componenti: Partially assembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi3218.PP1S131_40V6.1.

Structurei

3D structure databases

ProteinModelPortaliA9SX14.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini95 – 401PFKInterPro annotationAdd BLAST307

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni218 – 220Substrate bindingUniRule annotation3
Regioni263 – 265Substrate bindingUniRule annotation3
Regioni377 – 380Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2440. Eukaryota.
COG0205. LUCA.
InParanoidiA9SX14.
KOiK00850.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

A9SX14-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLNLNGTKV YTGAGGAILE DVPHLTDWLS GLPTYPNPLK YNPAYSAARQ
60 70 80 90 100
YFVEETDTVA KDVVVNINDV GHGIHFRRAG PRDQIYFKPE EVRACIVTCG
110 120 130 140 150
GLCPGINTVI REIVCGLWTQ YGVREILGID GGYRGFYSKN TIDLTPKTVN
160 170 180 190 200
DIHKRGGTVL QTSRGGHDLG KIVDSIQDRD INQVYIIGGD GTQKGANAIY
210 220 230 240 250
EECKRRGLKV AVAGIPKTID NDIDVIDKSF GFDTAVEEAQ RAINAAHVEA
260 270 280 290 300
ESVQNGLGLV KLMGRYSGYI AMHATLASRD VDCCLIPEVP FYLHGSGGLI
310 320 330 340 350
EFVRKRLRES GHMVIVVAEG AGQELMAEST VNNNDLKDAS GNKLLLDVGL
360 370 380 390 400
WLCTILKDHF QAEFRETLNL KYIDPKYMIR AIPSNASDNV YCTLLAHGAI
410 420 430 440 450
HGAMAGYSGF TVGPINNRHC YIPIKRVVAK SRNVNVADRM WARLMSSTNQ
460 470 480 490
PNFSRYEEVV QRQRKLNAQK QEEVENEKST LSSNGASKPV SLQNVGRQS
Length:499
Mass (Da):54,846
Last modified:February 5, 2008 - v1
Checksum:i1DFA57A86C2E3388
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS545020 Genomic DNA. Translation: EDQ64220.1.
RefSeqiXP_001770890.1. XM_001770838.1.
UniGeneiPpa.13928.

Genome annotation databases

GeneIDi5934162.
KEGGippp:PHYPADRAFT_136703.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS545020 Genomic DNA. Translation: EDQ64220.1.
RefSeqiXP_001770890.1. XM_001770838.1.
UniGeneiPpa.13928.

3D structure databases

ProteinModelPortaliA9SX14.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3218.PP1S131_40V6.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi5934162.
KEGGippp:PHYPADRAFT_136703.

Phylogenomic databases

eggNOGiKOG2440. Eukaryota.
COG0205. LUCA.
InParanoidiA9SX14.
KOiK00850.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiA9SX14_PHYPA
AccessioniPrimary (citable) accession number: A9SX14
Entry historyi
Integrated into UniProtKB/TrEMBL: February 5, 2008
Last sequence update: February 5, 2008
Last modified: July 6, 2016
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.