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Protein

1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2

Gene

PHYPADRAFT_134384

Organism
Physcomitrella patens subsp. patens (Moss)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of formate and 2-keto-4-methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).UniRule annotation

Catalytic activityi

1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 = 4-(methylthio)-2-oxobutanoate + formate.UniRule annotation

Cofactori

Fe cationUniRule annotationNote: Binds 1 Fe cation per monomer. Can also use other divalent metal cations.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 5 of the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Methylthioribose-1-phosphate isomerase (PHYPA_009170)
  2. Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 (PHYPA_003999), Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 (PHYPADRAFT_111231)
  3. Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 (PHYPA_003999), Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 (PHYPADRAFT_111231)
  4. Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 (PHYPA_003999), Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 (PHYPADRAFT_111231)
  5. 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 3 (PHYPADRAFT_164159), 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (PHYPA_014240), 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (PHYPA_003996), 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (PHYPA_018936), 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2 (PHYPADRAFT_134384), 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (PHYPA_030570), 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (PHYPA_001895), 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (PHYPA_006880), Uncharacterized protein (PHYPADRAFT_111075), 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (PHYPADRAFT_146733), 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 1 (PHYPADRAFT_128349), 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (PHYPA_017095), 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (PHYPADRAFT_71444)
  6. no protein annotated in this organism
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate, the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi94Iron or nickelUniRule annotation1
Metal bindingi96Iron or nickelUniRule annotation1
Metal bindingi100Iron or nickelUniRule annotation1
Metal bindingi139Iron or nickelUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processAmino-acid biosynthesis, Methionine biosynthesis
LigandIron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00904; UER00878

Names & Taxonomyi

Protein namesi
Recommended name:
1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2UniRule annotation (EC:1.13.11.54UniRule annotation)
Alternative name(s):
Acireductone dioxygenase (Fe(2+)-requiring) 2UniRule annotation
Short name:
ARD 2UniRule annotation
Short name:
Fe-ARD 2UniRule annotation
Gene namesi
ORF Names:PHYPADRAFT_134384
OrganismiPhyscomitrella patens subsp. patens (Moss)
Taxonomic identifieri3218 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaBryophytaBryophytinaBryopsidaFunariidaeFunarialesFunariaceaePhyscomitrella

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004143431 – 1951,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2Add BLAST195

Proteomic databases

PRIDEiA9SS00

Interactioni

Protein-protein interaction databases

STRINGi3218.PP1S111_19V6.1

Structurei

3D structure databases

ProteinModelPortaliA9SS00
SMRiA9SS00
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acireductone dioxygenase (ARD) family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2107 Eukaryota
COG1791 LUCA
InParanoidiA9SS00
KOiK08967

Family and domain databases

Gene3Di2.60.120.10, 1 hit
HAMAPiMF_03154 Salvage_MtnD_euk, 1 hit
InterProiView protein in InterPro
IPR004313 ARD
IPR027496 ARD_euk
IPR014710 RmlC-like_jellyroll
IPR011051 RmlC_Cupin_sf
PANTHERiPTHR23418 PTHR23418, 1 hit
PfamiView protein in Pfam
PF03079 ARD, 1 hit
SUPFAMiSSF51182 SSF51182, 1 hit

Sequencei

Sequence statusi: Complete.

A9SS00-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVQRPPLEA WYMNDSEEDQ RLPHHRNPPE YVTLEKLAAL GVIHWVLDAD
60 70 80 90 100
NHETDPELSI IRKDRGYNYT DVITVCPEML PSYEAKIKSF YEEHIHMDEE
110 120 130 140 150
IRYCLDGSGY FDVRDPEDHW IRIWVRKGDM IVLPAGCYHR FTLDEHNYIM
160 170 180 190
AMRLFVGEPI WTPYNRPQDE HPVRKGYVHQ FLQPELLDVD MSISA
Length:195
Mass (Da):23,199
Last modified:February 5, 2008 - v1
Checksum:iD0AED4C8E9A82C70
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS545000 Genomic DNA Translation: EDQ66042.1
RefSeqiXP_001769170.1, XM_001769118.1

Genome annotation databases

KEGGippp:PHYPADRAFT_134384

Similar proteinsi

Entry informationi

Entry nameiMTND2_PHYPA
AccessioniPrimary (citable) accession number: A9SS00
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 14, 2011
Last sequence update: February 5, 2008
Last modified: May 23, 2018
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

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