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Protein

Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme

Gene

glnE

Organism
Yersinia pestis bv. Antiqua (strain Angola)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.UniRule annotation

Catalytic activityi

[Glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP.UniRule annotation
ATP + [glutamine synthetase]-L-tyrosine = diphosphate + [glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine.UniRule annotation

Cofactori

Mg2+UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeUniRule annotation
Alternative name(s):
ATP:glutamine synthetase adenylyltransferaseUniRule annotation
ATaseUniRule annotation
Including the following 2 domains:
Glutamine synthetase adenylyl-L-tyrosine phosphorylaseUniRule annotation (EC:2.7.7.89UniRule annotation)
Alternative name(s):
Adenylyl removaseUniRule annotation
Short name:
ARUniRule annotation
Short name:
AT-NUniRule annotation
Glutamine synthetase adenylyl transferaseUniRule annotation (EC:2.7.7.42UniRule annotation)
Alternative name(s):
Adenylyl transferaseUniRule annotation
Short name:
ATUniRule annotation
Short name:
AT-CUniRule annotation
Gene namesi
Name:glnEUniRule annotation
Ordered Locus Names:YpAngola_A0293
OrganismiYersinia pestis bv. Antiqua (strain Angola)
Taxonomic identifieri349746 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001339281 – 951Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeAdd BLAST951

Structurei

3D structure databases

ProteinModelPortaliA9R7D6.
SMRiA9R7D6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 440Adenylyl removaseUniRule annotationAdd BLAST440
Regioni449 – 951Adenylyl transferaseUniRule annotationAdd BLAST503

Sequence similaritiesi

Belongs to the GlnE family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000256491.
KOiK00982.
OMAiEFMVQYA.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.

Sequencei

Sequence statusi: Complete.

A9R7D6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPLPSELQI QAQSIKQRFS ELPAPPDLRD EDIAVLALSD FVSDMLLIHP
60 70 80 90 100
QWLEELHQQP PQPQEWQYYS QWLSQALAGV QDEAALLTAL RLFRRRVMVR
110 120 130 140 150
IAWSQVLQTS GTAETLQQLS TLAESMIIAA RDWLYQVCCR ELGTPCNRQG
160 170 180 190 200
VPQPLLILGM GKLGGGELNF SSDIDLIFAY PENGQTQGGR RELDNAQFFT
210 220 230 240 250
RLGQRLIKAL DQHTIDGFVY RVDMRLRPFG DSGPLVLSFA ALEDYYQEQG
260 270 280 290 300
RDWERYAMVK ARLMGGADDP YSQELRQMLR PFVFRRYIDF SVIQSLRNMK
310 320 330 340 350
GMIAREVRRR GLKDNIKLGA GGIREIEFIT QVFQLIRGGR EPRLQERALL
360 370 380 390 400
PTLQAVAELG LLPEQQVADL SGSYLFLRRL ENLLQAIADE QTQTLPNDPL
410 420 430 440 450
NQARLAWGMG YADWAAMSTA LENHMQAVRV VFDDLIGDET PDIGEDPSHG
460 470 480 490 500
LYKSLWQDVL EESDLAPLTP HLEEAARRQL LATISGFRHD VDKRTIGPRG
510 520 530 540 550
REVLDQLMPR LFAEVCPRPD ANVALSRLIL LLLSIVTRTT YLELLVEYHA
560 570 580 590 600
ALKHVIRLCS ASPMVASQLA RYPLLLDELL DPQSLYQPLA PSAYRDELRQ
610 620 630 640 650
YLLRVPEDDE EQQLEALRQF KQAQQLRIAA GDITEALPVM KVSDHLTYLA
660 670 680 690 700
EAIIDAVIQQ AWNQMVARYG QPSHLQQSEG RGFAVIGYGK LGGWELGYSS
710 720 730 740 750
DLDLVFLLDC PLDVMTDGDR SIDGRQFYLR LAQRIMHLFS TRTSSGILYE
760 770 780 790 800
VDARLRPSGE AGMLVSTIEA FADYQRNEAW TWEHQALVRA RIVYGSPKLH
810 820 830 840 850
QQFDAIRQQI LCRHREDPQL QQEVREMREK MRNHLGSKQR DIFDIKADAG
860 870 880 890 900
GITDIEFIAQ YLVLRYAASE PRLTRWSDNV RIFESMAHYD IMSPEEAAAL
910 920 930 940 950
TRAYVTMRDE IHHLALQEQS SKVAADSFIA EREQVAASWH KWLAANDANV

S
Length:951
Mass (Da):108,330
Last modified:February 5, 2008 - v1
Checksum:iB9FFEF8FC128B97E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000901 Genomic DNA. Translation: ABX85091.1.
RefSeqiWP_002212194.1. NZ_CP009935.1.

Genome annotation databases

EnsemblBacteriaiABX85091; ABX85091; YpAngola_A0293.
KEGGiypg:YpAngola_A0293.
PATRICi18569472. VBIYerPes97331_0564.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000901 Genomic DNA. Translation: ABX85091.1.
RefSeqiWP_002212194.1. NZ_CP009935.1.

3D structure databases

ProteinModelPortaliA9R7D6.
SMRiA9R7D6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABX85091; ABX85091; YpAngola_A0293.
KEGGiypg:YpAngola_A0293.
PATRICi18569472. VBIYerPes97331_0564.

Phylogenomic databases

HOGENOMiHOG000256491.
KOiK00982.
OMAiEFMVQYA.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGLNE_YERPG
AccessioniPrimary (citable) accession number: A9R7D6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 5, 2008
Last modified: May 10, 2017
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.