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Protein

Pimeloyl-[acyl-carrier protein] methyl ester esterase

Gene

bioH

Organism
Yersinia pestis bv. Antiqua (strain Angola)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters.UniRule annotation

Catalytic activityi

Pimeloyl-[acyl-carrier protein] methyl ester + H2O = pimeloyl-[acyl-carrier protein] + methanol.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in the pathway biotin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei22 – 221Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation
Active sitei82 – 821NucleophileUniRule annotation
Active sitei207 – 2071UniRule annotation
Active sitei235 – 2351UniRule annotation
Binding sitei235 – 2351SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Keywords - Biological processi

Biotin biosynthesis

Enzyme and pathway databases

BioCyciYPES349746:GHPB-3758-MONOMER.
UniPathwayiUPA00078.

Protein family/group databases

ESTHERiyerpe-BIOH. BioH.

Names & Taxonomyi

Protein namesi
Recommended name:
Pimeloyl-[acyl-carrier protein] methyl ester esteraseUniRule annotation (EC:3.1.1.85UniRule annotation)
Alternative name(s):
Biotin synthesis protein BioHUniRule annotation
Carboxylesterase BioHUniRule annotation
Gene namesi
Name:bioHUniRule annotation
Ordered Locus Names:YpAngola_A3748
OrganismiYersinia pestis bv. Antiqua (strain Angola)
Taxonomic identifieri349746 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 258258Pimeloyl-[acyl-carrier protein] methyl ester esterasePRO_1000140013Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA9R4D0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni82 – 832Substrate bindingUniRule annotation
Regioni143 – 1475Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000028062.
KOiK02170.
OMAiLQDFADD.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01260. Carboxylester. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR010076. BioH.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01738. bioH. 1 hit.

Sequencei

Sequence statusi: Complete.

A9R4D0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQLYWYTCG EGDCDLVLLH GWGLNSGVWH CIIDRLAPHF RLHLVDLPGY
60 70 80 90 100
GRSQDYGAMS LADMAERVAQ QAPKQALWLG WSMGGLVASQ IALSQPECVR
110 120 130 140 150
GLITVSSSPC FTARDEWPGI KPEVLAGFQH QLSDDFHRTV ERFLALQTLG
160 170 180 190 200
TESSRQDARL LKSVVLQHQM PDVEVLTGGL AILRTADLRT ALAGFTLPFM
210 220 230 240 250
RVYGHLDSLV PRKVASLLDS AWPQTQSVVM QGAAHAPFIS HPNDFAKLIL

NFAEENKK
Length:258
Mass (Da):28,590
Last modified:February 5, 2008 - v1
Checksum:i5866E222A2C6D145
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000901 Genomic DNA. Translation: ABX86007.1.
RefSeqiWP_002208922.1. NZ_CP009935.1.

Genome annotation databases

EnsemblBacteriaiABX86007; ABX86007; YpAngola_A3748.
KEGGiypg:YpAngola_A3748.
PATRICi18577201. VBIYerPes97331_4351.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000901 Genomic DNA. Translation: ABX86007.1.
RefSeqiWP_002208922.1. NZ_CP009935.1.

3D structure databases

ProteinModelPortaliA9R4D0.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

ESTHERiyerpe-BIOH. BioH.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABX86007; ABX86007; YpAngola_A3748.
KEGGiypg:YpAngola_A3748.
PATRICi18577201. VBIYerPes97331_4351.

Phylogenomic databases

HOGENOMiHOG000028062.
KOiK02170.
OMAiLQDFADD.

Enzyme and pathway databases

UniPathwayiUPA00078.
BioCyciYPES349746:GHPB-3758-MONOMER.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01260. Carboxylester. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR010076. BioH.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01738. bioH. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBIOH_YERPG
AccessioniPrimary (citable) accession number: A9R4D0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 5, 2008
Last modified: September 7, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.