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A9QZR9

- THII_YERPG

UniProt

A9QZR9 - THII_YERPG

Protein

tRNA sulfurtransferase

Gene

thiI

Organism
Yersinia pestis bv. Antiqua (strain Angola)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (05 Feb 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotation

    Catalytic activityi

    L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.UniRule annotation
    [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei266 – 2661ATPUniRule annotation
    Binding sitei288 – 2881ATP; via amide nitrogenUniRule annotation
    Binding sitei297 – 2971ATPUniRule annotation
    Active sitei457 – 4571Cysteine persulfide intermediateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi184 – 1852ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. sulfurtransferase activity Source: UniProtKB-HAMAP
    3. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. thiamine biosynthetic process Source: UniProtKB-HAMAP
    2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
    3. thiazole biosynthetic process Source: InterPro
    4. tRNA thio-modification Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

    Enzyme and pathway databases

    BioCyciYPES349746:GHPB-3077-MONOMER.
    UniPathwayiUPA00060.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
    Alternative name(s):
    Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
    Thiamine biosynthesis protein ThiIUniRule annotation
    tRNA 4-thiouridine synthaseUniRule annotation
    Gene namesi
    Name:thiIUniRule annotation
    Ordered Locus Names:YpAngola_A3070
    OrganismiYersinia pestis bv. Antiqua (strain Angola)
    Taxonomic identifieri349746 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
    ProteomesiUP000001204: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 483483tRNA sulfurtransferasePRO_1000090047Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi345 ↔ 457Redox-activeUniRule annotation

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi349746.YpAngola_A3070.

    Structurei

    3D structure databases

    ProteinModelPortaliA9QZR9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini62 – 166105THUMPUniRule annotationAdd
    BLAST
    Domaini405 – 48379RhodaneseUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ThiI family.UniRule annotation
    Contains 1 rhodanese domain.UniRule annotation
    Contains 1 THUMP domain.UniRule annotation

    Keywords - Domaini

    Redox-active center

    Phylogenomic databases

    eggNOGiCOG0301.
    HOGENOMiHOG000227469.
    KOiK03151.
    OMAiKLFPEIM.
    OrthoDBiEOG6TBHGR.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    3.40.50.620. 1 hit.
    HAMAPiMF_00021. ThiI.
    InterProiIPR001763. Rhodanese-like_dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR026340. Thiazole_biosynth_dom.
    IPR020536. ThiI_AANH.
    IPR004114. THUMP.
    IPR003720. tRNA_STrfase.
    [Graphical view]
    PfamiPF02568. ThiI. 1 hit.
    PF02926. THUMP. 1 hit.
    [Graphical view]
    SMARTiSM00981. THUMP. 1 hit.
    [Graphical view]
    SUPFAMiSSF52821. SSF52821. 1 hit.
    TIGRFAMsiTIGR04271. ThiI_C_thiazole. 1 hit.
    TIGR00342. TIGR00342. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    PS51165. THUMP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A9QZR9-1 [UniParc]FASTAAdd to Basket

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    MKFIIKLFPE ITIKSQSVRL RFIKILTTNI RNVLKHLEDD TLAIVRHWDH    50
    IELRTKDDNL GPEICDALTR IPGIHHILEV EDRSYSDMHN IFEQTLEAYR 100
    ETLVGKTFCV RVKRRGKHEF SSGDVERYVG GGLNQHIESA KVNLTRPQVT 150
    VNLEVDQDKL ILVKARHEGL GGFPIGTQED VLSLISGGFD SGVSSYMLMR 200
    RGCRVHYCFF NLGGSAHEIG VKQVAHYLWN RFGSSHRVRF IAIDFEPVVG 250
    EILEKVEDGQ MGVVLKRMMV RAASQVAERY GVQALVTGEA LGQVSSQTLT 300
    NLRLIDNASD TLILRPLISH DKEHIINLAR QIGTEDFAKT MPEYCGVISK 350
    SPTVKAVKAK IEEEESHFDF SILDRVVSEA KNVDIREIAQ QSREQVVEVE 400
    TVAELADTDV LLDIRAPDEQ EEKPLKLDQV EVRSLPFYKL SSQFADLDQS 450
    KTYLLYCDRG VMSRLQALYL REQGYTNVKV YRP 483
    Length:483
    Mass (Da):54,894
    Last modified:February 5, 2008 - v1
    Checksum:iB773AAF42D9895BD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000901 Genomic DNA. Translation: ABX88049.1.
    RefSeqiYP_001607436.1. NC_010159.1.

    Genome annotation databases

    EnsemblBacteriaiABX88049; ABX88049; YpAngola_A3070.
    GeneIDi5801543.
    KEGGiypg:YpAngola_A3070.
    PATRICi18575696. VBIYerPes97331_3611.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000901 Genomic DNA. Translation: ABX88049.1 .
    RefSeqi YP_001607436.1. NC_010159.1.

    3D structure databases

    ProteinModelPortali A9QZR9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 349746.YpAngola_A3070.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABX88049 ; ABX88049 ; YpAngola_A3070 .
    GeneIDi 5801543.
    KEGGi ypg:YpAngola_A3070.
    PATRICi 18575696. VBIYerPes97331_3611.

    Phylogenomic databases

    eggNOGi COG0301.
    HOGENOMi HOG000227469.
    KOi K03151.
    OMAi KLFPEIM.
    OrthoDBi EOG6TBHGR.

    Enzyme and pathway databases

    UniPathwayi UPA00060 .
    BioCyci YPES349746:GHPB-3077-MONOMER.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    3.40.50.620. 1 hit.
    HAMAPi MF_00021. ThiI.
    InterProi IPR001763. Rhodanese-like_dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR026340. Thiazole_biosynth_dom.
    IPR020536. ThiI_AANH.
    IPR004114. THUMP.
    IPR003720. tRNA_STrfase.
    [Graphical view ]
    Pfami PF02568. ThiI. 1 hit.
    PF02926. THUMP. 1 hit.
    [Graphical view ]
    SMARTi SM00981. THUMP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52821. SSF52821. 1 hit.
    TIGRFAMsi TIGR04271. ThiI_C_thiazole. 1 hit.
    TIGR00342. TIGR00342. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    PS51165. THUMP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the deep-rooted Yersinia pestis strain Angola reveals new insights into the evolution and pangenome of the plague bacterium."
      Eppinger M., Worsham P.L., Nikolich M.P., Riley D.R., Sebastian Y., Mou S., Achtman M., Lindler L.E., Ravel J.
      J. Bacteriol. 192:1685-1699(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Angola.

    Entry informationi

    Entry nameiTHII_YERPG
    AccessioniPrimary (citable) accession number: A9QZR9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: February 5, 2008
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3