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A9Q1L1 (NSP4_ROTB2) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 18. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Non-structural glycoprotein 4

Short name=NSP4
OrganismRotavirus (isolate Human/Bangladesh/ADRV-N B219/2002) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-B219)) [Complete proteome]
Taxonomic identifier348136 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusunclassified rotaviruses
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length213 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in virus morphogenesis. Functions as a receptor for the immature double-layered inner capsid particle (ICP) which transiently buds into the lumen of the rough endoplasmic reticulum during viral maturation By similarity.

Enterotoxin that causes a phospholipase C-dependent elevation of the intracellular calcium concentration in host intestinal mucosa cells. Increased concentration of intracellular calcium disrupts the cytoskeleton and the tight junctions, raising the paracellular permeability. Potentiates chloride ion secretion through a calcium ion-dependent signaling pathway, inducing age-dependent diarrhea. To perform this enterotoxigenic role in vivo, NSP4 is probably released from infected enterocytes in a soluble form capable of diffusing within the intestinal lumen and interacting with the plasma membrane receptors on neighboring epithelial cells By similarity.

Subunit structure

Homotetramer By similarity.

Subcellular location

Non-structural glycoprotein 4: Host rough endoplasmic reticulum membrane; Single-pass type III membrane protein By similarity. Host membranehost caveola; Single-pass type III membrane protein. Secreted By similarity. Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles. NSP4 also localizes in vesicular structures, which contain an autophagosomal marker and associate with viroplasms in virus-infected cells By similarity.

Domain

The coiled coil region mediates oligomerization By similarity.

Sequence similarities

Belongs to the rotavirus NSP4 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 213213Non-structural glycoprotein 4
PRO_0000369865

Regions

Topological domain1 – 5555Lumenal By similarity
Transmembrane56 – 7217Helical; Potential
Topological domain73 – 213141Cytoplasmic By similarity
Coiled coil149 – 17527 Potential
Compositional bias124 – 1274Poly-Leu

Amino acid modifications

Glycosylation501N-linked (GlcNAc...); by host Potential

Sequences

Sequence LengthMass (Da)Tools
A9Q1L1 [UniParc].

Last modified February 5, 2008. Version 1.
Checksum: 262CC9CC256EABB6

FASTA21325,265
        10         20         30         40         50         60 
MEGTSESPIL DEFEANSNDY DNEFISRFSQ NPLHAFSLFT NGNIQEYFMN NSLEKIIIHI 

        70         80         90        100        110        120 
VLIIISLCGI KAQTSKIIYI VRLLFWKMYN VINNLVNKMI NREKIADRQI VDNRFREFEE 

       130        140        150        160        170        180 
RFRILLLQHD ENIAKQDNIV QYNKLDNFAE SIKSEFNLKV AEMERRFQEL KWRCDMIANK 

       190        200        210 
TMNTIVLTNT VDSTNKDEKI IFDEGSVVQY NRE 

« Hide

References

[1]"Whole genomic characterization of a human rotavirus strain B219 belonging to a novel group of the genus Rotavirus."
Nagashima S., Kobayashi N., Ishino M., Alam M.M., Ahmed M.U., Paul S.K., Ganesh B., Chawla-Sarkar M., Krishnan T., Naik T.N., Wang Y.-H.
J. Med. Virol. 80:2023-2033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EF453359 mRNA. Translation: ABR32126.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameNSP4_ROTB2
AccessionPrimary (citable) accession number: A9Q1L1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 5, 2008
Last modified: February 19, 2014
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families