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A9Q1L0 (VP4_ROTB2) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Outer capsid protein VP4
Alternative name(s):
Hemagglutinin

Cleaved into the following 2 chains:

  1. Outer capsid protein VP8*
  2. Outer capsid protein VP5*
OrganismRotavirus (isolate Human/Bangladesh/ADRV-N B219/2002) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-B219)) [Complete proteome]
Taxonomic identifier348136 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusunclassified rotaviruses
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length826 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors By similarity.

Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm By similarity.

VP8* forms the head of the spikes By similarity.

Subunit structure

VP4 is a homotrimer Potential.

Subcellular location

Outer capsid protein VP4: Virion By similarity. Host rough endoplasmic reticulum Potential. Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.

Outer capsid protein VP8*: Virion By similarity. Note: Outer capsid protein By similarity.

Outer capsid protein VP5*: Virion By similarity. Note: Outer capsid protein By similarity.

Post-translational modification

Proteolytic cleavage by trypsin results in activation of VP4 functions and greatly increases infectivity. The penetration into the host cell is dependent on trypsin treatment of VP4. It produces two peptides, VP5* and VP8* that remain associated with the virion By similarity.

Sequence similarities

Belongs to the rotavirus VP4 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 826826Outer capsid protein VP4
PRO_0000369842
Chain1 – 249249Outer capsid protein VP8* Potential
PRO_0000369843
Chain263 – 826564Outer capsid protein VP5* Potential
PRO_0000369844

Regions

Compositional bias260 – 2634Poly-Lys

Sites

Site249 – 2502Cleavage Potential
Site262 – 2632Cleavage Potential

Amino acid modifications

Glycosylation1431N-linked (GlcNAc...); by host Potential
Glycosylation1531N-linked (GlcNAc...); by host Potential
Glycosylation1981N-linked (GlcNAc...); by host Potential
Glycosylation3331N-linked (GlcNAc...); by host Potential
Glycosylation4871N-linked (GlcNAc...); by host Potential
Glycosylation4941N-linked (GlcNAc...); by host Potential
Glycosylation5141N-linked (GlcNAc...); by host Potential
Glycosylation5611N-linked (GlcNAc...); by host Potential
Glycosylation8041N-linked (GlcNAc...); by host Potential

Sequences

Sequence LengthMass (Da)Tools
A9Q1L0 [UniParc].

Last modified February 5, 2008. Version 1.
Checksum: B8CC3EDD7D540505

FASTA82693,311
        10         20         30         40         50         60 
MSLRSLLITT EAVGETTQTS DHQTSFSTRT YNEINDRPSL RVEKDGEKAY CFKNLDPVRY 

        70         80         90        100        110        120 
DTRMGEYPFD YGGQSTENNQ LQFDLFTKDL MADTDIGLSD DVRDDLKRQI KEYYQQGYRA 

       130        140        150        160        170        180 
IFLIRPQNQE QQYIASYSST NLNFTSQLSV GVNLSVLNKI QENKLHIYST QPHIPSVGCE 

       190        200        210        220        230        240 
MITKIFRTDV DNENSLINYS VPVTVTISVT KATFEDTFVW NQNNDYPNMN YKDLIPAVTK 

       250        260        270        280        290        300 
NSIYHDVKRI TKIHEYINSK KKKNGVGKIG GIQIAESKDG FWKILTKNYQ IKLKFGIEGY 

       310        320        330        340        350        360 
GVMGGTFGNW LIDSGFKTVE TNYEYQRNGK TINATTVASV KPSRKCGTRS PVFGQLQFSG 

       370        380        390        400        410        420 
EMMVLSHNDI LTVFYTEREW ALSNAIYAKN FATDFKRQFE VTAQSDELLV RTNVVPHTIK 

       430        440        450        460        470        480 
NTPGKALMEY SHGGFGQIDT SDYTGMALTF RFRCVSEDLP EGYYDKDKAL TFANVGLTSF 

       490        500        510        520        530        540 
QDRQETNGTY WVYNTSTVGF GSCYPKKEFE YDINVTYTTL LPSDPEFTTG GTNYAQSVTA 

       550        560        570        580        590        600 
VLEESFINLQ NQVNEMLTRM NISDLTSGVM SVFSVATSFP QILDGISDLL KAASSAFKKV 

       610        620        630        640        650        660 
KGKVGNVAKR LRGKRYVRLF DEDISIEETP RFLDSIRSSR RPSILSNMFN DDETFTALHT 

       670        680        690        700        710        720 
LASRTNSVAS DVTYIQPIIT TRIANSTPPV IAPASSVTYA KLKDISKIIN AEIDPKSIME 

       730        740        750        760        770        780 
FNQVSNTISI LDSTKKLAQY AVDPDVIDGI LNKMVGGHAR SLFSLKVRKH LLDAVEKDAF 

       790        800        810        820 
VKYNYHDLMG KLLNDRELLD ITNNLSSQKQ FELAKEFRDL LINALA 

« Hide

References

[1]"Whole genomic characterization of a human rotavirus strain B219 belonging to a novel group of the genus Rotavirus."
Nagashima S., Kobayashi N., Ishino M., Alam M.M., Ahmed M.U., Paul S.K., Ganesh B., Chawla-Sarkar M., Krishnan T., Naik T.N., Wang Y.-H.
J. Med. Virol. 80:2023-2033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PUTATIVE CLEAVAGE SITES.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EF453358 mRNA. Translation: ABR32125.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR000416. Haemagglutinin_VP4.
[Graphical view]
PfamPF00426. VP4_haemagglut. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameVP4_ROTB2
AccessionPrimary (citable) accession number: A9Q1L0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 5, 2008
Last modified: February 19, 2014
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families