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A9Q1L0

- VP4_ROTB2

UniProt

A9Q1L0 - VP4_ROTB2

Protein

Outer capsid protein VP4

Gene
N/A
Organism
Rotavirus (isolate Human/Bangladesh/ADRV-N B219/2002) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-B219))
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 29 (01 Oct 2014)
      Sequence version 1 (05 Feb 2008)
      Previous versions | rss
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    Functioni

    Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors By similarity.By similarity
    Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm By similarity.By similarity
    VP8* forms the head of the spikes.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei249 – 2502CleavageSequence Analysis
    Sitei262 – 2632CleavageSequence Analysis

    GO - Biological processi

    1. permeabilization of host organelle membrane involved in viral entry into host cell Source: UniProtKB-KW
    2. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hemagglutinin

    Keywords - Biological processi

    Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Viral penetration via permeabilization of host membrane, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Outer capsid protein VP4
    Alternative name(s):
    Hemagglutinin
    Cleaved into the following 2 chains:
    OrganismiRotavirus (isolate Human/Bangladesh/ADRV-N B219/2002) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-B219))
    Taxonomic identifieri348136 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusunclassified rotaviruses
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000008656: Genome

    Subcellular locationi

    Chain Outer capsid protein VP4 : Virion By similarity. Host rough endoplasmic reticulum Curated
    Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.By similarity
    Chain Outer capsid protein VP8* : Virion By similarity
    Note: Outer capsid protein.By similarity
    Chain Outer capsid protein VP5* : Virion By similarity
    Note: Outer capsid protein.By similarity

    GO - Cellular componenti

    1. host cell rough endoplasmic reticulum Source: UniProtKB-SubCell
    2. viral outer capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Host endoplasmic reticulum, Outer capsid protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 826826Outer capsid protein VP4PRO_0000369842Add
    BLAST
    Chaini1 – 249249Outer capsid protein VP8*Sequence AnalysisPRO_0000369843Add
    BLAST
    Chaini263 – 826564Outer capsid protein VP5*Sequence AnalysisPRO_0000369844Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi143 – 1431N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi153 – 1531N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi198 – 1981N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi333 – 3331N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi487 – 4871N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi494 – 4941N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi514 – 5141N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi561 – 5611N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi804 – 8041N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    Proteolytic cleavage by trypsin results in activation of VP4 functions and greatly increases infectivity. The penetration into the host cell is dependent on trypsin treatment of VP4. It produces two peptides, VP5* and VP8* that remain associated with the virion By similarity.By similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Glycoprotein

    Interactioni

    Subunit structurei

    VP4 is a homotrimer.Curated

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi260 – 2634Poly-Lys

    Sequence similaritiesi

    Belongs to the rotavirus VP4 family.Curated

    Family and domain databases

    InterProiIPR000416. Haemagglutinin_VP4.
    [Graphical view]
    PfamiPF00426. VP4_haemagglut. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A9Q1L0-1 [UniParc]FASTAAdd to Basket

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    MSLRSLLITT EAVGETTQTS DHQTSFSTRT YNEINDRPSL RVEKDGEKAY    50
    CFKNLDPVRY DTRMGEYPFD YGGQSTENNQ LQFDLFTKDL MADTDIGLSD 100
    DVRDDLKRQI KEYYQQGYRA IFLIRPQNQE QQYIASYSST NLNFTSQLSV 150
    GVNLSVLNKI QENKLHIYST QPHIPSVGCE MITKIFRTDV DNENSLINYS 200
    VPVTVTISVT KATFEDTFVW NQNNDYPNMN YKDLIPAVTK NSIYHDVKRI 250
    TKIHEYINSK KKKNGVGKIG GIQIAESKDG FWKILTKNYQ IKLKFGIEGY 300
    GVMGGTFGNW LIDSGFKTVE TNYEYQRNGK TINATTVASV KPSRKCGTRS 350
    PVFGQLQFSG EMMVLSHNDI LTVFYTEREW ALSNAIYAKN FATDFKRQFE 400
    VTAQSDELLV RTNVVPHTIK NTPGKALMEY SHGGFGQIDT SDYTGMALTF 450
    RFRCVSEDLP EGYYDKDKAL TFANVGLTSF QDRQETNGTY WVYNTSTVGF 500
    GSCYPKKEFE YDINVTYTTL LPSDPEFTTG GTNYAQSVTA VLEESFINLQ 550
    NQVNEMLTRM NISDLTSGVM SVFSVATSFP QILDGISDLL KAASSAFKKV 600
    KGKVGNVAKR LRGKRYVRLF DEDISIEETP RFLDSIRSSR RPSILSNMFN 650
    DDETFTALHT LASRTNSVAS DVTYIQPIIT TRIANSTPPV IAPASSVTYA 700
    KLKDISKIIN AEIDPKSIME FNQVSNTISI LDSTKKLAQY AVDPDVIDGI 750
    LNKMVGGHAR SLFSLKVRKH LLDAVEKDAF VKYNYHDLMG KLLNDRELLD 800
    ITNNLSSQKQ FELAKEFRDL LINALA 826
    Length:826
    Mass (Da):93,311
    Last modified:February 5, 2008 - v1
    Checksum:iB8CC3EDD7D540505
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF453358 mRNA. Translation: ABR32125.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF453358 mRNA. Translation: ABR32125.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR000416. Haemagglutinin_VP4.
    [Graphical view ]
    Pfami PF00426. VP4_haemagglut. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Whole genomic characterization of a human rotavirus strain B219 belonging to a novel group of the genus Rotavirus."
      Nagashima S., Kobayashi N., Ishino M., Alam M.M., Ahmed M.U., Paul S.K., Ganesh B., Chawla-Sarkar M., Krishnan T., Naik T.N., Wang Y.-H.
      J. Med. Virol. 80:2023-2033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PUTATIVE CLEAVAGE SITES.

    Entry informationi

    Entry nameiVP4_ROTB2
    AccessioniPrimary (citable) accession number: A9Q1L0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: February 5, 2008
    Last modified: October 1, 2014
    This is version 29 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3