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A9Q1K9

- VP3_ROTB2

UniProt

A9Q1K9 - VP3_ROTB2

Protein

Protein VP3

Gene
N/A
Organism
Rotavirus (isolate Human/Bangladesh/ADRV-N B219/2002) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-B219))
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 24 (01 Oct 2014)
      Sequence version 1 (05 Feb 2008)
      Previous versions | rss
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    Functioni

    Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't By similarity.By similarity

    Catalytic activityi

    GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
    S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
    3. mRNA guanylyltransferase activity Source: UniProtKB-EC
    4. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. viral process Source: InterPro

    Keywords - Molecular functioni

    Methyltransferase, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    mRNA capping, mRNA processing

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding, RNA-binding, S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein VP3
    Including the following 2 domains:
    mRNA guanylyltransferase (EC:2.7.7.50)
    mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
    OrganismiRotavirus (isolate Human/Bangladesh/ADRV-N B219/2002) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-B219))
    Taxonomic identifieri348136 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusunclassified rotaviruses
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000008656: Genome

    Subcellular locationi

    Virion Curated
    Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging Potential.Curated

    GO - Cellular componenti

    1. viral nucleocapsid Source: InterPro

    Keywords - Cellular componenti

    Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 719719Protein VP3PRO_0000369836Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with VP1. Interacts with VP2.Curated

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the rotavirus VP3 family.Curated

    Family and domain databases

    InterProiIPR011181. VP3_Rotav.
    [Graphical view]
    PfamiPF06929. Rotavirus_VP3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A9Q1K9-1 [UniParc]FASTAAdd to Basket

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    MAKLIIINSE KGEKVETHED IFKLSNLQQR EIYAITNERT KSILLNQTFY    50
    TILDIENEPK DRVAFDSYNS LFPTSIFSYN RQDRLFGTCN HVLDNNIHYS 100
    FALFDSMVDN LSTYLPNDWN IIKIPDSIDY PIGNDLLFYV FDNLVHMTID 150
    QFVNSEEKQM NTVPKCKESQ DRIKEVFTDI MSHLYMPAID YDPQSYNYRI 200
    SRREIGNLVR DQVFSLVKGH IHLIGPEMES LRNIIMFLHA GNSITFHTID 250
    TSKKSNYIKE LEFNKKTKLT MANVLINQRK NMNNFFKGLI KHYMTYGIPN 300
    KVYYIGAYPS YWLELITWVP FNIITYDPKY RHVDNDKIIW HDKLFDRNDI 350
    ETIESKSYIY IDIRTDIRKL DMTKKQRIFK EEDDMIVEIA TKLASKQCTV 400
    MFKRKIFPGN NMSFGDPLFH PKLTQLGREY YNCITTIVSP SIYKESELYS 450
    LLLSARSNNV SNYVYGGSKF DQSSIVNYNS TVIALYSLSN TVNSLETIEH 500
    AIKFNHIITF PHRTDRGDWR NIEELNNLSP FQNKKRQLEF EDWSIDPKNY 550
    AMKFGCEIVS ESVFLQLGHS RALIPDLYNH IISIRMEMPL FYPDRFFSHI 600
    GIRQPSIFKR DSYMTSRLSA YISRQLTHSI DLSVLKKNHF EGYSGHLIAI 650
    ETSFSSLVFT MSPYRWLIRA KKSLTKSKIR DKFKIGDGQP HTREEFENTY 700
    DYLKINRLVN STFHSLLLG 719
    Length:719
    Mass (Da):84,556
    Last modified:February 5, 2008 - v1
    Checksum:i6EE2CE8A87F5CC93
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF453357 mRNA. Translation: ABR32124.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF453357 mRNA. Translation: ABR32124.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR011181. VP3_Rotav.
    [Graphical view ]
    Pfami PF06929. Rotavirus_VP3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Whole genomic characterization of a human rotavirus strain B219 belonging to a novel group of the genus Rotavirus."
      Nagashima S., Kobayashi N., Ishino M., Alam M.M., Ahmed M.U., Paul S.K., Ganesh B., Chawla-Sarkar M., Krishnan T., Naik T.N., Wang Y.-H.
      J. Med. Virol. 80:2023-2033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiVP3_ROTB2
    AccessioniPrimary (citable) accession number: A9Q1K9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: February 5, 2008
    Last modified: October 1, 2014
    This is version 24 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3