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A9Q1K9

- VP3_ROTB2

UniProt

A9Q1K9 - VP3_ROTB2

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Protein
Protein VP3
Gene
N/A
Organism
Rotavirus (isolate Human/Bangladesh/ADRV-N B219/2002) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-B219))
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't By similarity.

Catalytic activityi

GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
  4. mRNA guanylyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. viral process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein VP3
Including the following 2 domains:
mRNA guanylyltransferase (EC:2.7.7.50)
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
OrganismiRotavirus (isolate Human/Bangladesh/ADRV-N B219/2002) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-B219))
Taxonomic identifieri348136 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusunclassified rotaviruses
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000008656: Genome

Subcellular locationi

Virion Reviewed prediction
Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging Reviewed prediction.

GO - Cellular componenti

  1. viral nucleocapsid Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 719719Protein VP3
PRO_0000369836Add
BLAST

Interactioni

Subunit structurei

Interacts with VP1 Reviewed prediction. Interacts with VP2 Reviewed prediction.

Family & Domainsi

Sequence similaritiesi

Belongs to the rotavirus VP3 family.

Family and domain databases

InterProiIPR011181. VP3_Rotav.
[Graphical view]
PfamiPF06929. Rotavirus_VP3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9Q1K9-1 [UniParc]FASTAAdd to Basket

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MAKLIIINSE KGEKVETHED IFKLSNLQQR EIYAITNERT KSILLNQTFY    50
TILDIENEPK DRVAFDSYNS LFPTSIFSYN RQDRLFGTCN HVLDNNIHYS 100
FALFDSMVDN LSTYLPNDWN IIKIPDSIDY PIGNDLLFYV FDNLVHMTID 150
QFVNSEEKQM NTVPKCKESQ DRIKEVFTDI MSHLYMPAID YDPQSYNYRI 200
SRREIGNLVR DQVFSLVKGH IHLIGPEMES LRNIIMFLHA GNSITFHTID 250
TSKKSNYIKE LEFNKKTKLT MANVLINQRK NMNNFFKGLI KHYMTYGIPN 300
KVYYIGAYPS YWLELITWVP FNIITYDPKY RHVDNDKIIW HDKLFDRNDI 350
ETIESKSYIY IDIRTDIRKL DMTKKQRIFK EEDDMIVEIA TKLASKQCTV 400
MFKRKIFPGN NMSFGDPLFH PKLTQLGREY YNCITTIVSP SIYKESELYS 450
LLLSARSNNV SNYVYGGSKF DQSSIVNYNS TVIALYSLSN TVNSLETIEH 500
AIKFNHIITF PHRTDRGDWR NIEELNNLSP FQNKKRQLEF EDWSIDPKNY 550
AMKFGCEIVS ESVFLQLGHS RALIPDLYNH IISIRMEMPL FYPDRFFSHI 600
GIRQPSIFKR DSYMTSRLSA YISRQLTHSI DLSVLKKNHF EGYSGHLIAI 650
ETSFSSLVFT MSPYRWLIRA KKSLTKSKIR DKFKIGDGQP HTREEFENTY 700
DYLKINRLVN STFHSLLLG 719
Length:719
Mass (Da):84,556
Last modified:February 5, 2008 - v1
Checksum:i6EE2CE8A87F5CC93
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF453357 mRNA. Translation: ABR32124.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF453357 mRNA. Translation: ABR32124.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR011181. VP3_Rotav.
[Graphical view ]
Pfami PF06929. Rotavirus_VP3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Whole genomic characterization of a human rotavirus strain B219 belonging to a novel group of the genus Rotavirus."
    Nagashima S., Kobayashi N., Ishino M., Alam M.M., Ahmed M.U., Paul S.K., Ganesh B., Chawla-Sarkar M., Krishnan T., Naik T.N., Wang Y.-H.
    J. Med. Virol. 80:2023-2033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiVP3_ROTB2
AccessioniPrimary (citable) accession number: A9Q1K9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 5, 2008
Last modified: April 16, 2014
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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