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Protein

Protein VP3

Gene
N/A
Organism
Rotavirus (isolate Human/Bangladesh/ADRV-N B219/2002) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-B219))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't (By similarity).By similarity

Catalytic activityi

GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
  3. mRNA guanylyltransferase activity Source: UniProtKB-EC
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. viral process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein VP3
Including the following 2 domains:
mRNA guanylyltransferase (EC:2.7.7.50)
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
OrganismiRotavirus (isolate Human/Bangladesh/ADRV-N B219/2002) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-B219))
Taxonomic identifieri348136 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusunclassified rotaviruses
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000008656: Genome

Subcellular locationi

Virion Curated
Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging (Potential).Curated

GO - Cellular componenti

  1. viral nucleocapsid Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 719719Protein VP3PRO_0000369836Add
BLAST

Interactioni

Subunit structurei

Interacts with VP1. Interacts with VP2.Curated

Family & Domainsi

Sequence similaritiesi

Belongs to the rotavirus VP3 family.Curated

Family and domain databases

InterProiIPR011181. VP3_Rotav.
[Graphical view]
PfamiPF06929. Rotavirus_VP3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9Q1K9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKLIIINSE KGEKVETHED IFKLSNLQQR EIYAITNERT KSILLNQTFY
60 70 80 90 100
TILDIENEPK DRVAFDSYNS LFPTSIFSYN RQDRLFGTCN HVLDNNIHYS
110 120 130 140 150
FALFDSMVDN LSTYLPNDWN IIKIPDSIDY PIGNDLLFYV FDNLVHMTID
160 170 180 190 200
QFVNSEEKQM NTVPKCKESQ DRIKEVFTDI MSHLYMPAID YDPQSYNYRI
210 220 230 240 250
SRREIGNLVR DQVFSLVKGH IHLIGPEMES LRNIIMFLHA GNSITFHTID
260 270 280 290 300
TSKKSNYIKE LEFNKKTKLT MANVLINQRK NMNNFFKGLI KHYMTYGIPN
310 320 330 340 350
KVYYIGAYPS YWLELITWVP FNIITYDPKY RHVDNDKIIW HDKLFDRNDI
360 370 380 390 400
ETIESKSYIY IDIRTDIRKL DMTKKQRIFK EEDDMIVEIA TKLASKQCTV
410 420 430 440 450
MFKRKIFPGN NMSFGDPLFH PKLTQLGREY YNCITTIVSP SIYKESELYS
460 470 480 490 500
LLLSARSNNV SNYVYGGSKF DQSSIVNYNS TVIALYSLSN TVNSLETIEH
510 520 530 540 550
AIKFNHIITF PHRTDRGDWR NIEELNNLSP FQNKKRQLEF EDWSIDPKNY
560 570 580 590 600
AMKFGCEIVS ESVFLQLGHS RALIPDLYNH IISIRMEMPL FYPDRFFSHI
610 620 630 640 650
GIRQPSIFKR DSYMTSRLSA YISRQLTHSI DLSVLKKNHF EGYSGHLIAI
660 670 680 690 700
ETSFSSLVFT MSPYRWLIRA KKSLTKSKIR DKFKIGDGQP HTREEFENTY
710
DYLKINRLVN STFHSLLLG
Length:719
Mass (Da):84,556
Last modified:February 5, 2008 - v1
Checksum:i6EE2CE8A87F5CC93
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF453357 mRNA. Translation: ABR32124.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF453357 mRNA. Translation: ABR32124.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR011181. VP3_Rotav.
[Graphical view]
PfamiPF06929. Rotavirus_VP3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Whole genomic characterization of a human rotavirus strain B219 belonging to a novel group of the genus Rotavirus."
    Nagashima S., Kobayashi N., Ishino M., Alam M.M., Ahmed M.U., Paul S.K., Ganesh B., Chawla-Sarkar M., Krishnan T., Naik T.N., Wang Y.-H.
    J. Med. Virol. 80:2023-2033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiVP3_ROTB2
AccessioniPrimary (citable) accession number: A9Q1K9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 5, 2008
Last modified: January 7, 2015
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.