Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A9Q1K8 (VP2_ROTB2) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 16. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Protein attributes

Sequence length973 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Inner capsid protein that self assembles to form an icosahedral capsid with a T=2 symmetry, which consists of 120 copies of VP2, with channels at each of its five-fold vertices. This capsid constitutes the innermost concentric layer of the viral mature particle. It encapsidates the polymerase VP1, the capping enzyme VP3 and the genomic dsRNA, thereby defining the core. The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nacent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels at the five-fold axes. VP2 is required for the replicase activity of VP1 polymerase. It probably plays a role in the coordination of packaging and genome replication by controlling the initiation of minus-strand synthesis. Binding to the polymerase VP1 presumably activates the autoinhibited VP1-RNA complex which will start the synthesis of the complementary minus-strand By similarity.

Subunit structure

Dimer Potential. Interacts with VP1. Interacts with VP3. Interacts with NSP5 By similarity.

Subcellular location

Virion Potential. Note: Inner capsid protein. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.

Sequence similarities

Belongs to the rotavirus VP2 family.

Ontologies

Keywords
   Cellular componentCapsid protein
Inner capsid protein
Virion
   LigandRNA-binding
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentviral inner capsid

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 973973Inner capsid protein VP2
PRO_0000369834

Regions

Region449 – 46921Hydrophobic

Sequences

Sequence LengthMass (Da)Tools
A9Q1K8 [UniParc].

Last modified February 5, 2008. Version 1.
Checksum: 03B08088108FBFD6

FASTA973111,085
        10         20         30         40         50         60 
MEVIEKLETI RETLNDTKDK KDFQKIHDEL VQYLDGVDSL TIDDDKWNEI LKLFKTIIIK 

        70         80         90        100        110        120 
LKSSTIKTTP LENELLQHEK KRTVKEVEKV EEDIKTDVTN DTSKPTLMDK VLQVSNQPNN 

       130        140        150        160        170        180 
PYSADVLQIR TILSKTLFVD TDSEAYSLYV PESQKLDVSP ITIELTTIEK YQPKVNILKQ 

       190        200        210        220        230        240 
AVIVPSQNPL LADTYGAPEI LFSTDFFDDI TSNSSEGLQL YFFDKAYKLK KELPNLPFLS 

       250        260        270        280        290        300 
SLDKDVNPLN PLNSVCKSFG QEKYYDMVMD RTDRGLDARR AAMQFDNVIV DAQNRTVQFN 

       310        320        330        340        350        360 
VRMHPFDLQL LRISQQFAEP MQDLAPVVRE YMMLGADGYV LTQKIRLDRD QQLIANRRSV 

       370        380        390        400        410        420 
VFDRMCELSG PLYRSRIIHS MRMMSKLWRT NVFRTSLEDE ITKIYAAAEV SMISIDATTS 

       430        440        450        460        470        480 
ALSTINIASA EQTLNALLNM SFFRCELDLI GSQSSFGAAM SAMIALMILP TDQENMDDEV 

       490        500        510        520        530        540 
FDVLCNLVYN ELIAWAADRP VFVRRAGATN AFRQFVNAGL NRDITNYMRF VLLRRPWLPL 

       550        560        570        580        590        600 
YNSRDVRRNA HVLVPNVDLA NINDQVYVAI NSFLNGIIEA SRRNPNPNKT ISANSFRKLM 

       610        620        630        640        650        660 
KNMRDICVNR LMPVIRLIRY NVERIGMILH MLPYSADIFD INRNLRDERL RIKIPMSGFL 

       670        680        690        700        710        720 
SLVMGITKAP DAFDWSQILN FADDVRKMDY AEAISIEDSA SVAIMRNDAN RATSKKEIFI 

       730        740        750        760        770        780 
SEVRPPTPTV ASIQKIPSAT LTAIFSDRQL INLIRDTHSF RVIREIAVAL QAAFDNSPTS 

       790        800        810        820        830        840 
QHGVGKGAVL HPVPQNFGRS SQFVRRDNIL LQRPAGIQQF TIEDLKQGRY FQGLMAQIRA 

       850        860        870        880        890        900 
RQPIIVNGPI PLRISDAAEI EQVTLAFLTM NSPYDAYIDP RDLKQQKLLT DREVDLFIDQ 

       910        920        930        940        950        960 
SPARPNDEFD NVMARTSVFI IDAPRAIVPI NPQRLNFPYH DIMVTDSVTK FIEFTVALTP 

       970 
DLQLFNGLLV FEQ 

« Hide

References

[1]"Whole genomic characterization of a human rotavirus strain B219 belonging to a novel group of the genus Rotavirus."
Nagashima S., Kobayashi N., Ishino M., Alam M.M., Ahmed M.U., Paul S.K., Ganesh B., Chawla-Sarkar M., Krishnan T., Naik T.N., Wang Y.-H.
J. Med. Virol. 80:2023-2033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EF453356 mRNA. Translation: ABR32123.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameVP2_ROTB2
AccessionPrimary (citable) accession number: A9Q1K8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 5, 2008
Last modified: October 16, 2013
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families