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A9Q1K7

- RDRP_ROTB2

UniProt

A9Q1K7 - RDRP_ROTB2

Protein

RNA-directed RNA polymerase

Gene
N/A
Organism
Rotavirus (isolate Human/Bangladesh/ADRV-N B219/2002) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-B219))
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 24 (01 Oct 2014)
      Sequence version 1 (05 Feb 2008)
      Previous versions | rss
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    Functioni

    RNA-directed RNA polymerase that is involved in both transcription and genome replication. Together with VP3 capping enzyme, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. One copy of each of the viral (+)RNAs is also recruited during core assembly, together with newly synthesized polymerase complexes and VP2. The polymerase of these novo-formed particles catalyzes the synthesis of complementary minus-strands leading to dsDNA formation. To do so, the polymerase specifically recognizes conserved 3' sequence(s) in plus-strand RNA templates. Once dsRNA synthesis is complete, the polymerase switches to the transcriptional mode, thus providing secondary transcription By similarity.PROSITE-ProRule annotation

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

    GO - Molecular functioni

    1. nucleotide binding Source: UniProtKB-KW
    2. RNA binding Source: UniProtKB-KW
    3. RNA-directed RNA polymerase activity Source: UniProtKB-KW

    GO - Biological processi

    1. transcription, DNA-templated Source: InterPro
    2. viral genome replication Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

    Keywords - Biological processi

    Viral RNA replication

    Keywords - Ligandi

    Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA-directed RNA polymerase (EC:2.7.7.48)
    Alternative name(s):
    Protein VP1
    OrganismiRotavirus (isolate Human/Bangladesh/ADRV-N B219/2002) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-B219))
    Taxonomic identifieri348136 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusunclassified rotaviruses
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000008656: Genome

    Subcellular locationi

    Virion Curated
    Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.By similarity

    GO - Cellular componenti

    1. virion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11671167RNA-directed RNA polymerasePRO_0000369830Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with VP3 Potential. Interacts with VP2 Potential. Interacts with NSP5; this interaction is probably necessary for the formation of functional virus factories By similarity.By similarityCurated

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini553 – 735183RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR001795. RNA-dir_pol_luteovirus.
    IPR007097. RNA-dir_pol_reovirus.
    [Graphical view]
    PfamiPF02123. RdRP_4. 1 hit.
    [Graphical view]
    PROSITEiPS50523. RDRP_DSRNA_REO. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A9Q1K7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPENYLAWL ARDIVRNLSY TSLVYNNPKV AIVELLDNKE AFFTYEKEQK     50
    TPEALINYID SIVKSSISVE DKIEALLKIR YISVYVDDKS DKRDIVLQLL 100
    NRTIKKIESK TKISNELNDA INAITIESRN WKIQNSESFK PYHYNQLVSD 150
    FLKYNEFEIL EGTDPLKWKS DTLQGLSPNY NHRTHTLISS IIYATSVRFD 200
    NYNDEQLQVL LYLFSIIKTN YVNGYLEILP NRKWSHSLAD LRENKSIMMY 250
    SAKIIHASCA MISILHAVPI DYFFLAQIIA SFSEIPAHAA KQLSSPMTLY 300
    IGIAQLRSNI VVSTKIAAES VATESPNISR LEESQLREWE QEMNEYPFQS 350
    SRMVRMMKKN IFDVSVDVFY AIFNCFSATF HVGHRIDNPQ DAIEAQVKVE 400
    YTSDVDKEMY DQYYFLLKRM LTDQLAEYAE EMYFKYNSDV TAESLAAMAN 450
    SSNGYSRSVT FIDREIKTTK KMLHLDDDLS KNLNFTNIGE QIKKGIPMGT 500
    RNVPARQTRG IFILSWQVAA IQHTIAEFLY KKAKKGGFGA TFAEAYVSKA 550
    ATLTYGILAE ATSKADQLIL YTDVSQWDAS QHNTEPYRSA WINAIKEART 600
    KYKINYNQEP VVLGMNVLDK MIEIQEALLN SNLIVESQGS KRQPLRIKYH 650
    GVASGEKTTK IGNSFANVAL ITTVFNNLTN TMPSIRVNHM RVDGDDNVVT 700
    MYTANRIDEV QENIKEKYKR MNAKVKALAS YTGLEMAKRF IICGKIFERG 750
    AISIFTAERP YGTDLSVQST TGSLIYSAAV NAYRGFGDDY LNFMTDVLVP 800
    PSASVKITGR LRSLLSPVTL YSTGPLSFEI TPYGLGGRMR LFSLSKENME 850
    LYKILTSSLA ISIQPDEIKK YSSTPQFKAR VDRMISSVQI AMKSEAKIIT 900
    SILRDKEEQK TLGVPNVATA KNRQQIDKAR KTLSLPKEIL PKVTKYYPEE 950
    IFHLILRNST LTIPKLNTMT KVYMNNSVNI TKLQQQIGVR VSSGIQVHKP 1000
    INTLLKLVEK HSPIKISPSD LILYSKKYDL TNLNGKKQFL MDLGISGNEL 1050
    RFYLNSKLLF HDLLLSKYDK LYEAPGFGAT QLNALPLDLT AAEKVFSIKL 1100
    NLPNTYYELL MLVLLYEYVN FVMFTGNTFR AVCIPESQTI NAKLVKTIMT 1150
    MIDNIQLDTV MFSDNIF 1167
    Length:1,167
    Mass (Da):132,901
    Last modified:February 5, 2008 - v1
    Checksum:iC612426A9D1FE310
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF453355 mRNA. Translation: ABR32122.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF453355 mRNA. Translation: ABR32122.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR001795. RNA-dir_pol_luteovirus.
    IPR007097. RNA-dir_pol_reovirus.
    [Graphical view ]
    Pfami PF02123. RdRP_4. 1 hit.
    [Graphical view ]
    PROSITEi PS50523. RDRP_DSRNA_REO. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Whole genomic characterization of a human rotavirus strain B219 belonging to a novel group of the genus Rotavirus."
      Nagashima S., Kobayashi N., Ishino M., Alam M.M., Ahmed M.U., Paul S.K., Ganesh B., Chawla-Sarkar M., Krishnan T., Naik T.N., Wang Y.-H.
      J. Med. Virol. 80:2023-2033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiRDRP_ROTB2
    AccessioniPrimary (citable) accession number: A9Q1K7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: February 5, 2008
    Last modified: October 1, 2014
    This is version 24 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3