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A9Q1K7

- RDRP_ROTB2

UniProt

A9Q1K7 - RDRP_ROTB2

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Protein

RNA-directed RNA polymerase

Gene
N/A
Organism
Rotavirus (isolate Human/Bangladesh/ADRV-N B219/2002) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-B219))
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli

Functioni

RNA-directed RNA polymerase that is involved in both transcription and genome replication. Together with VP3 capping enzyme, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. One copy of each of the viral (+)RNAs is also recruited during core assembly, together with newly synthesized polymerase complexes and VP2. The polymerase of these novo-formed particles catalyzes the synthesis of complementary minus-strands leading to dsDNA formation. To do so, the polymerase specifically recognizes conserved 3' sequence(s) in plus-strand RNA templates. Once dsRNA synthesis is complete, the polymerase switches to the transcriptional mode, thus providing secondary transcription (By similarity).PROSITE-ProRule annotation

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

GO - Molecular functioni

  1. nucleotide binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. RNA-directed RNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. transcription, DNA-templated Source: InterPro
  2. viral genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase (EC:2.7.7.48)
Alternative name(s):
Protein VP1
OrganismiRotavirus (isolate Human/Bangladesh/ADRV-N B219/2002) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-B219))
Taxonomic identifieri348136 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusunclassified rotaviruses
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000008656: Genome

Subcellular locationi

Virion Curated
Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging (By similarity).By similarity

GO - Cellular componenti

  1. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11671167RNA-directed RNA polymerasePRO_0000369830Add
BLAST

Interactioni

Subunit structurei

Interacts with VP3 (Potential). Interacts with VP2 (Potential). Interacts with NSP5; this interaction is probably necessary for the formation of functional virus factories (By similarity).By similarityCurated

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini553 – 735183RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR001795. RNA-dir_pol_luteovirus.
IPR007097. RNA-dir_pol_reovirus.
[Graphical view]
PfamiPF02123. RdRP_4. 1 hit.
[Graphical view]
PROSITEiPS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9Q1K7-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MEPENYLAWL ARDIVRNLSY TSLVYNNPKV AIVELLDNKE AFFTYEKEQK
60 70 80 90 100
TPEALINYID SIVKSSISVE DKIEALLKIR YISVYVDDKS DKRDIVLQLL
110 120 130 140 150
NRTIKKIESK TKISNELNDA INAITIESRN WKIQNSESFK PYHYNQLVSD
160 170 180 190 200
FLKYNEFEIL EGTDPLKWKS DTLQGLSPNY NHRTHTLISS IIYATSVRFD
210 220 230 240 250
NYNDEQLQVL LYLFSIIKTN YVNGYLEILP NRKWSHSLAD LRENKSIMMY
260 270 280 290 300
SAKIIHASCA MISILHAVPI DYFFLAQIIA SFSEIPAHAA KQLSSPMTLY
310 320 330 340 350
IGIAQLRSNI VVSTKIAAES VATESPNISR LEESQLREWE QEMNEYPFQS
360 370 380 390 400
SRMVRMMKKN IFDVSVDVFY AIFNCFSATF HVGHRIDNPQ DAIEAQVKVE
410 420 430 440 450
YTSDVDKEMY DQYYFLLKRM LTDQLAEYAE EMYFKYNSDV TAESLAAMAN
460 470 480 490 500
SSNGYSRSVT FIDREIKTTK KMLHLDDDLS KNLNFTNIGE QIKKGIPMGT
510 520 530 540 550
RNVPARQTRG IFILSWQVAA IQHTIAEFLY KKAKKGGFGA TFAEAYVSKA
560 570 580 590 600
ATLTYGILAE ATSKADQLIL YTDVSQWDAS QHNTEPYRSA WINAIKEART
610 620 630 640 650
KYKINYNQEP VVLGMNVLDK MIEIQEALLN SNLIVESQGS KRQPLRIKYH
660 670 680 690 700
GVASGEKTTK IGNSFANVAL ITTVFNNLTN TMPSIRVNHM RVDGDDNVVT
710 720 730 740 750
MYTANRIDEV QENIKEKYKR MNAKVKALAS YTGLEMAKRF IICGKIFERG
760 770 780 790 800
AISIFTAERP YGTDLSVQST TGSLIYSAAV NAYRGFGDDY LNFMTDVLVP
810 820 830 840 850
PSASVKITGR LRSLLSPVTL YSTGPLSFEI TPYGLGGRMR LFSLSKENME
860 870 880 890 900
LYKILTSSLA ISIQPDEIKK YSSTPQFKAR VDRMISSVQI AMKSEAKIIT
910 920 930 940 950
SILRDKEEQK TLGVPNVATA KNRQQIDKAR KTLSLPKEIL PKVTKYYPEE
960 970 980 990 1000
IFHLILRNST LTIPKLNTMT KVYMNNSVNI TKLQQQIGVR VSSGIQVHKP
1010 1020 1030 1040 1050
INTLLKLVEK HSPIKISPSD LILYSKKYDL TNLNGKKQFL MDLGISGNEL
1060 1070 1080 1090 1100
RFYLNSKLLF HDLLLSKYDK LYEAPGFGAT QLNALPLDLT AAEKVFSIKL
1110 1120 1130 1140 1150
NLPNTYYELL MLVLLYEYVN FVMFTGNTFR AVCIPESQTI NAKLVKTIMT
1160
MIDNIQLDTV MFSDNIF
Length:1,167
Mass (Da):132,901
Last modified:February 5, 2008 - v1
Checksum:iC612426A9D1FE310
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF453355 mRNA. Translation: ABR32122.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF453355 mRNA. Translation: ABR32122.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR001795. RNA-dir_pol_luteovirus.
IPR007097. RNA-dir_pol_reovirus.
[Graphical view ]
Pfami PF02123. RdRP_4. 1 hit.
[Graphical view ]
PROSITEi PS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Whole genomic characterization of a human rotavirus strain B219 belonging to a novel group of the genus Rotavirus."
    Nagashima S., Kobayashi N., Ishino M., Alam M.M., Ahmed M.U., Paul S.K., Ganesh B., Chawla-Sarkar M., Krishnan T., Naik T.N., Wang Y.-H.
    J. Med. Virol. 80:2023-2033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiRDRP_ROTB2
AccessioniPrimary (citable) accession number: A9Q1K7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 5, 2008
Last modified: October 29, 2014
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3