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A9Q1D5 (TOP1M_PANTR) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase I, mitochondrial

Short name=TOP1mt
EC=5.99.1.2
Gene names
Name:TOP1MT
OrganismPan troglodytes (Chimpanzee) [Reference proteome]
Taxonomic identifier9598 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePan

Protein attributes

Sequence length601 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Cofactor

Divalent metal ions (calcium or magnesium) By similarity.

Subcellular location

Mitochondrion By similarity.

Sequence similarities

Belongs to the type IB topoisomerase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5050Mitochondrion By similarity
Chain51 – 601551DNA topoisomerase I, mitochondrial
PRO_0000384394

Regions

Region261 – 2622Interaction with DNA By similarity
Region324 – 3296Interaction with DNA By similarity
Region421 – 4233Interaction with DNA By similarity

Sites

Active site5591O-(3'-phospho-DNA)-tyrosine intermediate By similarity
Site1521Interaction with DNA By similarity
Site2001Interaction with DNA By similarity
Site2481Interaction with DNA By similarity
Site2791Interaction with DNA By similarity
Site3371Interaction with DNA By similarity
Site3681Interaction with DNA By similarity
Site4101Interaction with DNA By similarity
Site4681Interaction with DNA By similarity
Site4861Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
A9Q1D5 [UniParc].

Last modified February 5, 2008. Version 1.
Checksum: F8064FE822DB84F3

FASTA60169,758
        10         20         30         40         50         60 
MRVVRLLRLR AALTLLGEVP RRPASRGVPG SRRTQKGSGA RWEKEKHEDG VKWRQLEHKG 

        70         80         90        100        110        120 
PYFAPPYEPL PDGVRFFYEG KPVRLSVAAE EVATFYGRML GHEYTTKEVF RKNFFNDWRK 

       130        140        150        160        170        180 
EMAVEEREVI KSLDKCDFTE IHRYFVDKAA ARKVLSREEK QKLKEEAEKL QREFGYCILD 

       190        200        210        220        230        240 
GHQEKIGNFK IEPPGLFRGR GDHPKMGMLK RRIMPEDVVI NCSRDSKIPE PPAGHQWKEV 

       250        260        270        280        290        300 
RSDNTVTWLA AWTESVQNSI KYIMLNPCSK LKGETAWQKF ETARRLRGFV DEIRSQYRAD 

       310        320        330        340        350        360 
WKSREMKTRQ RAVALYFIDK LALRAGNEKE DGEAADTVGC CSLRVEHVQL HPEADGCQHV 

       370        380        390        400        410        420 
VEFDFLGKDC IRYYNRVPVE KPVYKNLQLF MESKGPRDNL FDRLTTTSLN KHLQELMDGL 

       430        440        450        460        470        480 
TAKVFRTYNA SITLQEQLRA LTRAEDSIAA KILSYNRANR VVAILCNHQR ATPSTFEKSM 

       490        500        510        520        530        540 
QNLQTKIQAK KEQVAEARAE LRRARAEHKA QGDGKSRSVL EKKRRLLEKL QEQLAQLSVQ 

       550        560        570        580        590        600 
ATDKEENKQV ALGTSKLNYL DPRISIAWCK RFRVPVEKIY SKTQRERFAW ALAMAGEDFE 


F 

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References

[1]"Chimpanzee mitochondrial topoisomerase I."
Zhang H., Pommier Y.
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EF421830 mRNA. Translation: ABP57788.1.
RefSeqNP_001106268.1. NM_001112797.1.
UniGenePtr.6282.

3D structure databases

ProteinModelPortalA9Q1D5.
SMRA9Q1D5. Positions 42-601.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9598.ENSPTRP00000035312.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID472883.
KEGGptr:472883.

Organism-specific databases

CTD116447.

Phylogenomic databases

eggNOGCOG3569.
HOGENOMHOG000105469.
InParanoidA9Q1D5.
KOK03163.

Family and domain databases

Gene3D1.10.10.41. 1 hit.
1.10.132.10. 1 hit.
2.170.11.10. 2 hits.
3.90.15.10. 1 hit.
InterProIPR011010. DNA_brk_join_enz.
IPR013034. DNA_topo_domain1.
IPR001631. TopoI.
IPR018521. TopoI_AS.
IPR025834. TopoI_C_dom.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR014727. TopoI_cat_a/b-sub_euk.
IPR013500. TopoI_cat_euk.
IPR008336. TopoI_DNA-bd_euk.
IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
IPR013499. TopoI_euk.
[Graphical view]
PfamPF14370. Topo_C_assoc. 1 hit.
PF01028. Topoisom_I. 1 hit.
PF02919. Topoisom_I_N. 1 hit.
[Graphical view]
PRINTSPR00416. EUTPISMRASEI.
SMARTSM00435. TOPEUc. 1 hit.
[Graphical view]
SUPFAMSSF56349. SSF56349. 2 hits.
SSF56741. SSF56741. 1 hit.
PROSITEPS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20849513.

Entry information

Entry nameTOP1M_PANTR
AccessionPrimary (citable) accession number: A9Q1D5
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: February 5, 2008
Last modified: July 9, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families