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A9Q1D5

- TOP1M_PANTR

UniProt

A9Q1D5 - TOP1M_PANTR

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Protein

DNA topoisomerase I, mitochondrial

Gene
TOP1MT
Organism
Pan troglodytes (Chimpanzee)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Cofactori

Divalent metal ions (calcium or magnesium) By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei152 – 1521Interaction with DNA By similarity
Sitei200 – 2001Interaction with DNA By similarity
Sitei248 – 2481Interaction with DNA By similarity
Sitei279 – 2791Interaction with DNA By similarity
Sitei337 – 3371Interaction with DNA By similarity
Sitei368 – 3681Interaction with DNA By similarity
Sitei410 – 4101Interaction with DNA By similarity
Sitei468 – 4681Interaction with DNA By similarity
Sitei486 – 4861Interaction with DNA By similarity
Active sitei559 – 5591O-(3'-phospho-DNA)-tyrosine intermediate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: RefGenome
  4. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro

GO - Biological processi

  1. DNA replication Source: RefGenome
  2. DNA topological change Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase I, mitochondrial (EC:5.99.1.2)
Short name:
TOP1mt
Gene namesi
Name:TOP1MT
OrganismiPan troglodytes (Chimpanzee)
Taxonomic identifieri9598 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePan
ProteomesiUP000002277: Unplaced

Subcellular locationi

Mitochondrion By similarity

GO - Cellular componenti

  1. chromosome Source: InterPro
  2. mitochondrial nucleoid Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5050Mitochondrion By similarityAdd
BLAST
Chaini51 – 601551DNA topoisomerase I, mitochondrialPRO_0000384394Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi9598.ENSPTRP00000035312.

Structurei

3D structure databases

ProteinModelPortaliA9Q1D5.
SMRiA9Q1D5. Positions 42-601.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni261 – 2622Interaction with DNA By similarity
Regioni324 – 3296Interaction with DNA By similarity
Regioni421 – 4233Interaction with DNA By similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG3569.
HOGENOMiHOG000105469.
InParanoidiA9Q1D5.
KOiK03163.

Family and domain databases

Gene3Di1.10.10.41. 1 hit.
1.10.132.10. 1 hit.
2.170.11.10. 2 hits.
3.90.15.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR013034. DNA_topo_domain1.
IPR001631. TopoI.
IPR018521. TopoI_AS.
IPR025834. TopoI_C_dom.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR014727. TopoI_cat_a/b-sub_euk.
IPR013500. TopoI_cat_euk.
IPR008336. TopoI_DNA-bd_euk.
IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
IPR013499. TopoI_euk.
[Graphical view]
PfamiPF14370. Topo_C_assoc. 1 hit.
PF01028. Topoisom_I. 1 hit.
PF02919. Topoisom_I_N. 1 hit.
[Graphical view]
PRINTSiPR00416. EUTPISMRASEI.
SMARTiSM00435. TOPEUc. 1 hit.
[Graphical view]
SUPFAMiSSF56349. SSF56349. 2 hits.
SSF56741. SSF56741. 1 hit.
PROSITEiPS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A9Q1D5-1 [UniParc]FASTAAdd to Basket

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MRVVRLLRLR AALTLLGEVP RRPASRGVPG SRRTQKGSGA RWEKEKHEDG    50
VKWRQLEHKG PYFAPPYEPL PDGVRFFYEG KPVRLSVAAE EVATFYGRML 100
GHEYTTKEVF RKNFFNDWRK EMAVEEREVI KSLDKCDFTE IHRYFVDKAA 150
ARKVLSREEK QKLKEEAEKL QREFGYCILD GHQEKIGNFK IEPPGLFRGR 200
GDHPKMGMLK RRIMPEDVVI NCSRDSKIPE PPAGHQWKEV RSDNTVTWLA 250
AWTESVQNSI KYIMLNPCSK LKGETAWQKF ETARRLRGFV DEIRSQYRAD 300
WKSREMKTRQ RAVALYFIDK LALRAGNEKE DGEAADTVGC CSLRVEHVQL 350
HPEADGCQHV VEFDFLGKDC IRYYNRVPVE KPVYKNLQLF MESKGPRDNL 400
FDRLTTTSLN KHLQELMDGL TAKVFRTYNA SITLQEQLRA LTRAEDSIAA 450
KILSYNRANR VVAILCNHQR ATPSTFEKSM QNLQTKIQAK KEQVAEARAE 500
LRRARAEHKA QGDGKSRSVL EKKRRLLEKL QEQLAQLSVQ ATDKEENKQV 550
ALGTSKLNYL DPRISIAWCK RFRVPVEKIY SKTQRERFAW ALAMAGEDFE 600
F 601
Length:601
Mass (Da):69,758
Last modified:February 5, 2008 - v1
Checksum:iF8064FE822DB84F3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF421830 mRNA. Translation: ABP57788.1.
RefSeqiNP_001106268.1. NM_001112797.1.
UniGeneiPtr.6282.

Genome annotation databases

GeneIDi472883.
KEGGiptr:472883.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF421830 mRNA. Translation: ABP57788.1 .
RefSeqi NP_001106268.1. NM_001112797.1.
UniGenei Ptr.6282.

3D structure databases

ProteinModelPortali A9Q1D5.
SMRi A9Q1D5. Positions 42-601.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9598.ENSPTRP00000035312.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 472883.
KEGGi ptr:472883.

Organism-specific databases

CTDi 116447.

Phylogenomic databases

eggNOGi COG3569.
HOGENOMi HOG000105469.
InParanoidi A9Q1D5.
KOi K03163.

Miscellaneous databases

NextBioi 20849513.

Family and domain databases

Gene3Di 1.10.10.41. 1 hit.
1.10.132.10. 1 hit.
2.170.11.10. 2 hits.
3.90.15.10. 1 hit.
InterProi IPR011010. DNA_brk_join_enz.
IPR013034. DNA_topo_domain1.
IPR001631. TopoI.
IPR018521. TopoI_AS.
IPR025834. TopoI_C_dom.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR014727. TopoI_cat_a/b-sub_euk.
IPR013500. TopoI_cat_euk.
IPR008336. TopoI_DNA-bd_euk.
IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
IPR013499. TopoI_euk.
[Graphical view ]
Pfami PF14370. Topo_C_assoc. 1 hit.
PF01028. Topoisom_I. 1 hit.
PF02919. Topoisom_I_N. 1 hit.
[Graphical view ]
PRINTSi PR00416. EUTPISMRASEI.
SMARTi SM00435. TOPEUc. 1 hit.
[Graphical view ]
SUPFAMi SSF56349. SSF56349. 2 hits.
SSF56741. SSF56741. 1 hit.
PROSITEi PS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Chimpanzee mitochondrial topoisomerase I."
    Zhang H., Pommier Y.
    Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiTOP1M_PANTR
AccessioniPrimary (citable) accession number: A9Q1D5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: February 5, 2008
Last modified: July 9, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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