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A9Q1D5

- TOP1M_PANTR

UniProt

A9Q1D5 - TOP1M_PANTR

Protein

DNA topoisomerase I, mitochondrial

Gene

TOP1MT

Organism
Pan troglodytes (Chimpanzee)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (05 Feb 2008)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.By similarity

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

    Cofactori

    Divalent metal ions (calcium or magnesium).By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei152 – 1521Interaction with DNABy similarity
    Sitei200 – 2001Interaction with DNABy similarity
    Sitei248 – 2481Interaction with DNABy similarity
    Sitei279 – 2791Interaction with DNABy similarity
    Sitei337 – 3371Interaction with DNABy similarity
    Sitei368 – 3681Interaction with DNABy similarity
    Sitei410 – 4101Interaction with DNABy similarity
    Sitei468 – 4681Interaction with DNABy similarity
    Sitei486 – 4861Interaction with DNABy similarity
    Active sitei559 – 5591O-(3'-phospho-DNA)-tyrosine intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: RefGenome
    3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro

    GO - Biological processi

    1. DNA replication Source: RefGenome
    2. DNA topological change Source: RefGenome

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase I, mitochondrial (EC:5.99.1.2)
    Short name:
    TOP1mt
    Gene namesi
    Name:TOP1MT
    OrganismiPan troglodytes (Chimpanzee)
    Taxonomic identifieri9598 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePan
    ProteomesiUP000002277: Unplaced

    Subcellular locationi

    Mitochondrion By similarity

    GO - Cellular componenti

    1. chromosome Source: InterPro
    2. mitochondrial nucleoid Source: RefGenome

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5050MitochondrionBy similarityAdd
    BLAST
    Chaini51 – 601551DNA topoisomerase I, mitochondrialPRO_0000384394Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi9598.ENSPTRP00000035312.

    Structurei

    3D structure databases

    ProteinModelPortaliA9Q1D5.
    SMRiA9Q1D5. Positions 42-601.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni261 – 2622Interaction with DNABy similarity
    Regioni324 – 3296Interaction with DNABy similarity
    Regioni421 – 4233Interaction with DNABy similarity

    Sequence similaritiesi

    Belongs to the type IB topoisomerase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG3569.
    HOGENOMiHOG000105469.
    InParanoidiA9Q1D5.
    KOiK03163.

    Family and domain databases

    Gene3Di1.10.10.41. 1 hit.
    1.10.132.10. 1 hit.
    2.170.11.10. 2 hits.
    3.90.15.10. 1 hit.
    InterProiIPR011010. DNA_brk_join_enz.
    IPR013034. DNA_topo_domain1.
    IPR001631. TopoI.
    IPR018521. TopoI_AS.
    IPR025834. TopoI_C_dom.
    IPR014711. TopoI_cat_a-hlx-sub_euk.
    IPR014727. TopoI_cat_a/b-sub_euk.
    IPR013500. TopoI_cat_euk.
    IPR008336. TopoI_DNA-bd_euk.
    IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
    IPR013499. TopoI_euk.
    [Graphical view]
    PfamiPF14370. Topo_C_assoc. 1 hit.
    PF01028. Topoisom_I. 1 hit.
    PF02919. Topoisom_I_N. 1 hit.
    [Graphical view]
    PRINTSiPR00416. EUTPISMRASEI.
    SMARTiSM00435. TOPEUc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56349. SSF56349. 2 hits.
    SSF56741. SSF56741. 1 hit.
    PROSITEiPS00176. TOPOISOMERASE_I_EUK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A9Q1D5-1 [UniParc]FASTAAdd to Basket

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    MRVVRLLRLR AALTLLGEVP RRPASRGVPG SRRTQKGSGA RWEKEKHEDG    50
    VKWRQLEHKG PYFAPPYEPL PDGVRFFYEG KPVRLSVAAE EVATFYGRML 100
    GHEYTTKEVF RKNFFNDWRK EMAVEEREVI KSLDKCDFTE IHRYFVDKAA 150
    ARKVLSREEK QKLKEEAEKL QREFGYCILD GHQEKIGNFK IEPPGLFRGR 200
    GDHPKMGMLK RRIMPEDVVI NCSRDSKIPE PPAGHQWKEV RSDNTVTWLA 250
    AWTESVQNSI KYIMLNPCSK LKGETAWQKF ETARRLRGFV DEIRSQYRAD 300
    WKSREMKTRQ RAVALYFIDK LALRAGNEKE DGEAADTVGC CSLRVEHVQL 350
    HPEADGCQHV VEFDFLGKDC IRYYNRVPVE KPVYKNLQLF MESKGPRDNL 400
    FDRLTTTSLN KHLQELMDGL TAKVFRTYNA SITLQEQLRA LTRAEDSIAA 450
    KILSYNRANR VVAILCNHQR ATPSTFEKSM QNLQTKIQAK KEQVAEARAE 500
    LRRARAEHKA QGDGKSRSVL EKKRRLLEKL QEQLAQLSVQ ATDKEENKQV 550
    ALGTSKLNYL DPRISIAWCK RFRVPVEKIY SKTQRERFAW ALAMAGEDFE 600
    F 601
    Length:601
    Mass (Da):69,758
    Last modified:February 5, 2008 - v1
    Checksum:iF8064FE822DB84F3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF421830 mRNA. Translation: ABP57788.1.
    RefSeqiNP_001106268.1. NM_001112797.1.
    UniGeneiPtr.6282.

    Genome annotation databases

    GeneIDi472883.
    KEGGiptr:472883.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF421830 mRNA. Translation: ABP57788.1 .
    RefSeqi NP_001106268.1. NM_001112797.1.
    UniGenei Ptr.6282.

    3D structure databases

    ProteinModelPortali A9Q1D5.
    SMRi A9Q1D5. Positions 42-601.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9598.ENSPTRP00000035312.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 472883.
    KEGGi ptr:472883.

    Organism-specific databases

    CTDi 116447.

    Phylogenomic databases

    eggNOGi COG3569.
    HOGENOMi HOG000105469.
    InParanoidi A9Q1D5.
    KOi K03163.

    Miscellaneous databases

    NextBioi 20849513.

    Family and domain databases

    Gene3Di 1.10.10.41. 1 hit.
    1.10.132.10. 1 hit.
    2.170.11.10. 2 hits.
    3.90.15.10. 1 hit.
    InterProi IPR011010. DNA_brk_join_enz.
    IPR013034. DNA_topo_domain1.
    IPR001631. TopoI.
    IPR018521. TopoI_AS.
    IPR025834. TopoI_C_dom.
    IPR014711. TopoI_cat_a-hlx-sub_euk.
    IPR014727. TopoI_cat_a/b-sub_euk.
    IPR013500. TopoI_cat_euk.
    IPR008336. TopoI_DNA-bd_euk.
    IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
    IPR013499. TopoI_euk.
    [Graphical view ]
    Pfami PF14370. Topo_C_assoc. 1 hit.
    PF01028. Topoisom_I. 1 hit.
    PF02919. Topoisom_I_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00416. EUTPISMRASEI.
    SMARTi SM00435. TOPEUc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56349. SSF56349. 2 hits.
    SSF56741. SSF56741. 1 hit.
    PROSITEi PS00176. TOPOISOMERASE_I_EUK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Chimpanzee mitochondrial topoisomerase I."
      Zhang H., Pommier Y.
      Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiTOP1M_PANTR
    AccessioniPrimary (citable) accession number: A9Q1D5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: February 5, 2008
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3