A9PEK8 (METK3_POPTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
S-adenosylmethionine synthase 3
Short name=AdoMet synthase 3
EC=2.5.1.6

Alternative name(s):
Methionine adenosyltransferase 3
Short name=MAT 3

Gene names

Name:

METK3

Organism

Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa)

Taxonomic identifier

3694 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Malpighiales › Salicaceae › Saliceae › Populus

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme By similarity.
Catalytic activity	ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.
Cofactor	Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. Binds 1 potassium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the AdoMet synthase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Transferase
Gene Ontology (GO)
Biological process	S-adenosylmethionine biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine adenosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	S-adenosylmethionine synthase 3		PRO_0000363044
Regions
	Nucleotide binding	120 – 125	6	ATP Potential
	Nucleotide binding	268 – 275	8	ATP Potential
Sites
	Metal binding	18	1	Magnesium By similarity
	Metal binding	44	1	Potassium By similarity
	Metal binding	272	1	Potassium By similarity
	Metal binding	280	1	Magnesium By similarity
	Binding site	148	1	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A9PEK8 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 8F826163D0CA6F31	FASTA	395	43,170
	10 20 30 40 50 60 MAETFLFTSE SVNEGHPDKL CDQISDAVLD ACLAQDPDSK VACETCTKTN MVMVFGEITT 70 80 90 100 110 120 KADVDYEKIV RDTCRNIGFT SADVGLDADN CKVLVNIEQQ SPDIAQGVHG HFSKRPEEIG 130 140 150 160 170 180 AGDQGHMFGY ATDETPELMP LSHVLATKLG ARLTEVRKNG TCAWLRPDGK TQVTVEYYNE 190 200 210 220 230 240 NGAMVPVRVH TVLISTQHDE TVTNDEIAAD LKEHVIKPVI PEKYLDEKTI FHLNPSGRFV 250 260 270 280 290 300 IGGPHGDAGL TGRKIIIDTY GGWGAHGGGA FSGKDPTKVD RSGAYIVRQA AKSIVASGLA 310 320 330 340 350 360 RRCIVQVSYA IGVPEPLSVF VDTYGTGKIP DKEILQIVKE SFDFRPGMIS INLDLKRGGN 370 380 390 SRFLKTAAYG HFGRDDPDFT WEVVKPLKWD NKVQA

References

[1]

"The poplar transcriptome: Analysis of ca. 4,700 sequence-verified full-length cDNAs." Ralph S.G., Chun H.J.E., Cooper D., Kirkpatrick R., Palmquist D., Wynhoven B., Kolosova N., Oddy C., Jancsik S., Douglas C.J., Liu J., Butterfield Y.S.N., Stott J., Yang G., Holt R.A., Siddiqui A., Jones S.J.M., Marra M.A., Ritland K., Bohlmann J. Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nisqually. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EF146764 mRNA. Translation: ABK94811.1.
3D structure databases
ProteinModelPortal	A9PEK8.
SMR	A9PEK8. Positions 4-389.
ModBase	Search...
Protein-protein interaction databases
STRING	A9PEK8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	POPTR_0008s09870.2; POPTR_0008s09870.2; POPTR_0008s09870.
Family and domain databases
InterPro	IPR022631. ADOMET_SYNTHASE_CS. IPR022630. S-AdoMet_synt_C. IPR022629. S-AdoMet_synt_central. IPR022628. S-AdoMet_synt_N. IPR002133. S-AdoMet_synthetase. IPR022636. S-AdoMet_synthetase_sfam. [Graphical view]
PANTHER	PTHR11964. S-AdoMet_synt. 1 hit.
Pfam	PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000497. MAT. 1 hit.
SUPFAM	SSF55973. S-AdoMet_synt. 3 hits.
TIGRFAMs	TIGR01034. MetK. 1 hit.
PROSITE	PS00376. ADOMET_SYNTHASE_1. 1 hit. PS00377. ADOMET_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METK3_POPTR

Accession

Primary (citable) accession number: A9PEK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	February 5, 2008
Last modified:	November 16, 2011
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program