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A9NNH7

- LIAS_PICSI

UniProt

A9NNH7 - LIAS_PICSI

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Protein
Lipoyl synthase, mitochondrial
Gene
LIP1
Organism
Picea sitchensis (Sitka spruce) (Pinus sitchensis)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi115 – 1151Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi120 – 1201Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi126 – 1261Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi146 – 1461Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi150 – 1501Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi153 – 1531Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrial (EC:2.8.1.8)
Alternative name(s):
Lipoate synthase
Short name:
LS
Short name:
Lip-syn
Lipoic acid synthase
Gene namesi
Name:LIP1
OrganismiPicea sitchensis (Sitka spruce) (Pinus sitchensis)
Taxonomic identifieri3332 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaePinalesPinaceaePicea

Subcellular locationi

Mitochondrion By similarity UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 386Lipoyl synthase, mitochondrialUniRule annotationPRO_0000398853
Transit peptidei1 – ?Mitochondrion Reviewed prediction

Structurei

3D structure databases

ProteinModelPortaliA9NNH7.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
MF_03128. Lipoyl_synth_plantM.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR027527. Lipoyl_synth_mt.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A9NNH7-1 [UniParc]FASTAAdd to Basket

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MSINPAFLRR ITWGSKSLHH QFTRCQVRAL SSSVEQTPES TTPALSALRE    50
RLANGGPTLS DFLTRNLGEE PYSVDVGTKK NPLPKPKWMK AAVPGGDKYT 100
AIKAKLREMN LHTVCEEAKC PNLGECWSGG ETGTATATIM ILGDTCTRGC 150
RFCAVKTSRT PPPPDPNEPT NVAEAIVSWG LDYVVLTSVD RDDLPDQGSG 200
HFAKTVQKLK QLKPKMLVEA LVPDFQGNSE CVQKVATSGL DVFAHNIETV 250
EELQRVVRDH RANFNQSLEV LKMAKTYSPL GVLTKTSVML GCGETPAQVI 300
ETMEKVREAG VDVITFGQYM RPTKRHMAVS EYVTPEAFEK YQKLGMEMGF 350
RYVASGPMVR SSYKAGEFYI KSMIEDDRKK ASSSSI 386
Length:386
Mass (Da):42,579
Last modified:February 5, 2008 - v1
Checksum:i0FAA92A285A950F8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF082836 mRNA. Translation: ABK22188.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF082836 mRNA. Translation: ABK22188.1 .

3D structure databases

ProteinModelPortali A9NNH7.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
MF_03128. Lipoyl_synth_plantM.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR027527. Lipoyl_synth_mt.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "A conifer genomics resource of 200,000 spruce (Picea spp.) ESTs and 6,464 high-quality, sequence-finished full-length cDNAs for Sitka spruce (Picea sitchensis)."
    Ralph S.G., Chun H.J., Kolosova N., Cooper D., Oddy C., Ritland C.E., Kirkpatrick R., Moore R., Barber S., Holt R.A., Jones S.J., Marra M.A., Douglas C.J., Ritland K., Bohlmann J.
    BMC Genomics 9:484-484(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiLIAS_PICSI
AccessioniPrimary (citable) accession number: A9NNH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: February 5, 2008
Last modified: March 19, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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