A9NHF4 (SYY_ACHLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine--tRNA ligase
EC=6.1.1.1

Alternative name(s):
Tyrosyl-tRNA synthetase
Short name=TyrRS

Gene names

Name:	tyrS
Ordered Locus Names:	ACL_1185

Organism

Acholeplasma laidlawii (strain PG-8A) [Complete proteome] [HAMAP]

Taxonomic identifier

441768 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Acholeplasma

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP MF_02006
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02006
Subunit structure	Homodimer By similarity. HAMAP MF_02006
Subcellular location	Cytoplasm By similarity HAMAP MF_02006.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 410	410	Tyrosine--tRNA ligase HAMAP MF_02006		PRO_1000088572
Regions
	Domain	344 – 410	67	S4 RNA-binding
	Motif	39 – 48	10	"HIGH" region HAMAP MF_02006
	Motif	224 – 228	5	"KMSKS" region HAMAP MF_02006
Sites
	Binding site	34	1	Tyrosine By similarity
	Binding site	163	1	Tyrosine By similarity
	Binding site	167	1	Tyrosine By similarity
	Binding site	227	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9NHF4 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: C76193B6508E8CF9	FASTA	410	46,427
	10 20 30 40 50 60 MDLFKQLEYR GMVKDVSDLE LAKQLLNEQK IKFYVGYDPT GESLTVGHLV QIVRMLYLQK 70 80 90 100 110 120 HGHTPVVLIG GGTGLIGDPR ETSERSLLTL EKSLENAQKI ENQIKGLIKG NVEFVNNYEW 130 140 150 160 170 180 LSKIDLISFL RDYGKHFSVS YMLHKDTVQK RLESGISYTE FSYMILQSID WLNLYQNRDV 190 200 210 220 230 240 KIQFGGSDQW GNLTSGLELI RRVLGKNDAV GLSSPLLLKA DGTKFGKSES GALWLDKELT 250 260 270 280 290 300 SPYELYQYFL NSSDTDIEQF LKLLTLIEQD EIEAILETHR EKPELRHAQK VLAKAVVELV 310 320 330 340 350 360 HGKEELETAL QVTEALFSGE FNGLTKSAYK TLESVIDVVR FNGESLIELV VKSKLASSNR 370 380 390 400 410 EAREFISNGA ISIQGEKITD SMYNVGETSK AFDTYAIIRR GKKKYALIVF

References

[1]

"Complete genome and proteome of Acholeplasma laidlawii." Lazarev V.N., Levitskii S.A., Basovskii Y.I., Chukin M.M., Akopian T.A., Vereshchagin V.V., Kostrjukova E.S., Kovaleva G.Y., Kazanov M.D., Malko D.B., Vitreschak A.G., Sernova N.V., Gelfand M.S., Demina I.A., Serebryakova M.V., Galyamina M.A., Vtyurin N.N., Rogov S.I. , Alexeev D.G., Ladygina V.G., Govorun V.M. J. Bacteriol. 193:4943-4953(2011) [PubMed: 21784942] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PG-8A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000896 Genomic DNA. Translation: ABX81784.1.
RefSeq	YP_001621160.1. NC_010163.1.
3D structure databases
ProteinModelPortal	A9NHF4.
ModBase	Search...
Protein-protein interaction databases
STRING	A9NHF4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5803682.
GenomeReviews	Gene locus ACL_1185 in contig CP000896_GR.
KEGG	acl:ACL_1185.
PATRIC	20639068. VBIAchLai134690_1140.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG288125.
OMA	TFYIGFD.
Enzyme and pathway databases
BioCyc	ALAI441768:ACL_1185-MONOMER.
Family and domain databases
HAMAP	MF_02006. Tyr_tRNA_synth_type1. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA-bd. IPR002307. Tyr-tRNA-synth. IPR024088. Tyr-tRNA-synth_bac-type. IPR024107. Tyr-tRNA-synth_bac_1. [Graphical view]
Gene3D	G3DSA:3.10.290.10. G3DSA:3.10.290.10. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01866.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF01479. S4. 1 hit. PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00234. TyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY_ACHLI

Accession

Primary (citable) accession number: A9NHF4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program