Reviewed, UniProtKB/Swiss-Prot A9NGE2 (PNP_ACHLI)

Names and origin

Protein names

Recommended name:
    Polyribonucleotide nucleotidyltransferase
    EC=2.7.7.8
Alternative name(s):
    Polynucleotide phosphorylase
      Short name=PNPase

Gene names

Name:	pnp
Ordered Locus Names:	ACL_0808

Organism

Acholeplasma laidlawii (strain PG-8A) [Complete proteome] [HAMAP]

Taxonomic identifier

441768 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Acholeplasma

Protein attributes

Sequence length	715 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.
Catalytic activity	RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP MF_01595
Subunit structure	Homotrimer. Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the polyribonucleotide nucleotidyltransferase family. Contains 1 KH domain. Contains 1 S1 motif domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro mRNA catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW polyribonucleotide nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 715	715	Polyribonucleotide nucleotidyltransferase HAMAP MF_01595		PRO_0000329476
Regions
	Domain	567 – 634	68	KH
	Domain	637 – 712	76	S1 motif

Sequences

	Sequence		Length	Mass (Da)
	A9NGE2-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 0A03C34B64B5E270	FASTA	715	78,666
	10 20 30 40 50 60 MSKQVFETTL AGKPLRVEVG EIAKQANGAA MIYYGDTVVL STAVAKAKVG QTDFFPLMVI 70 80 90 100 110 120 YAEKQYAAGK IPGGFFRREG RPSEVETLTS RLIDRPLRPL FDDGYRNEVQ VVNTVLSSDP 130 140 150 160 170 180 EASSQMAAML GSSIALEISN IPFMGPIAGA HVGRIDGQFV LNPSSEQLNV SDIDLIVAGT 190 200 210 220 230 240 KDAINMVEAG AKQVSEEVML EAILFGHEAI KELCAFQETI KKAFGVEKVE PELLSLNQAI 250 260 270 280 290 300 FDEVFAYKGK ELVKAVSLVD KQERYDVIDA IKDEVVAHFE QRNFWMTVDG KEVLDADQKK 310 320 330 340 350 360 ELMNQVKVSL DRIVTKEVRR LITEDKVRPD GRGLDEIRPL ASSVDLLPRT HGSALFTRGQ 370 380 390 400 410 420 TQALGIVTLG SLNENQIIDG LEQETVTKRF MLHYNFPPFS VGETGRYGAP GRREIGHGAL 430 440 450 460 470 480 GERALLQVLP SEDEFPYAIR VVSEITESNG SSSQATICVG SMALMAAGVP IKAPVAGIAM 490 500 510 520 530 540 GLIMDGEHYS ILSDIQGMED HEGDMDFKVA GTKDGITALQ MDIKIQGITT EIMKEALEQA 550 560 570 580 590 600 RKGRLHILSH MNTVISETRT ELSAFAPKVK MIRINPDKIR DVIGAGGKII TQIIEDHNNV 610 620 630 640 650 660 KIDIEQDGRV FIMHTDSAWL NKTAAYIESL VREAKVGELY EAKVTRLLMD KDGKKIQGVF 670 680 690 700 710 AEIFPGTEGL VHISKWEKER TESLDGKVKV GDQILVKVVK IDERGRVDLS RKDAL

References

[1]

"Acholeplasma laidlawii complete genome." Kovaleva G.Y., Kazanov M.D., Malko D.B., Vitreschak A.G., Sernova N.V., Gelfand M.S., Lazarev V.N., Levitskii S.A., Basovskii Y.I., Chukin M.M., Akopian T.A., Vereshchagin V.A., Kostrjukova E.S., Selezneva O.V., Vtyurin N.N., Rogov S.I., Alekseev D.G., Ladygina V.G. , Titova G.A., Karpov V.A., Govorun V.M. Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000896 Genomic DNA. Translation: ABX81422.1.
RefSeq	YP_001620798.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5803780.
GenomeReviews	Gene locus ACL_0808 in contig CP000896_GR.
KEGG	acl:ACL_0808.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A9NGE2. GHGNLAK.
Family and domain databases
HAMAP	MF_01595. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR004087. KH. IPR018111. KH_type_1_subgr. IPR012340. NA-bd_OB-fold. IPR012162. PNPase. IPR015848. PNPase_PH_RNA-bd_bac/org-type. IPR003029. Rbsml_prot_S1_RNA-bd_dom. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:1.10.10.400. PNPase_PH_RNA-bd_bac/org-type. 1 hit.
PANTHER	PTHR11252. PNPase. 1 hit.
Pfam	PF00013. KH_1. 1 hit. PF03726. PNPase. 1 hit. PF01138. RNase_PH. 2 hits. PF03725. RNase_PH_C. 2 hits. PF00575. S1. 1 hit. [Graphical view]
PIRSF	PIRSF005499. PNPase. 1 hit.
SMART	SM00322. KH. 1 hit. [Graphical view]
TIGRFAMs	TIGR03591. Polynuc_phos. 1 hit.
PROSITE	PS50084. KH_TYPE_1. False negative. PS50126. S1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PNP_ACHLI

Accession

Primary (citable) accession number: A9NGE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	February 5, 2008
Last modified:	June 16, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)