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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Acholeplasma laidlawii (strain PG-8A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei543Aminoacyl-adenylateUniRule annotation1
Binding sitei587ATPUniRule annotation1
Metal bindingi869ZincUniRule annotation1
Metal bindingi872ZincUniRule annotation1
Metal bindingi885ZincUniRule annotation1
Metal bindingi888ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciALAI441768:G1GAM-715-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:ACL_0702
OrganismiAcholeplasma laidlawii (strain PG-8A)
Taxonomic identifieri441768 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesAcholeplasmatalesAcholeplasmataceaeAcholeplasma
Proteomesi
  • UP000008558 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002162311 – 896Isoleucine--tRNA ligaseAdd BLAST896

Proteomic databases

PRIDEiA9NG38

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi441768.ACL_0702

Structurei

3D structure databases

ProteinModelPortaliA9NG38
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi584 – 588"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A9NG38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYKDTLLMP KTDFEMRGNL GKREPEIQKH WESIDLYNKV LEKNKDNTPF
60 70 80 90 100
VLHDGPPYAN NNIHIGHAFQ KTLKDFVLRY QTMSGRYTPY IPGWDTHGLP
110 120 130 140 150
IENEVTKSGV DRKQLTRAEF RKICREYAIK QVEVQRAQFK RLGILGDWDN
160 170 180 190 200
PYLTLDKSYI ADQVRVFSQM VDKGIIYKGL KPIYWSPSSE SAFAEAEIEY
210 220 230 240 250
MDKQSKSIYV GFDMMGDKFP NTKLLIWTTT PWTLPANLAV SVHPNFDYVW
260 270 280 290 300
FNNDGKNYIA LKELLPKLVE KLGFSHAKVL KEFKGSTLEF MNYKHPLYDR
310 320 330 340 350
VSPIILGEHV TAEDGTGLVH TAPGHGEDDY FVGKKYNLDL LSPVDEKGHM
360 370 380 390 400
TEEAGPYAGM FYEKANAQIV EDLRENGHLL YDETITHSYP HDWRTKKPVI
410 420 430 440 450
FRSTPQWFAS IDMLKGDLLE AIKGVKWHTS WGEVRLTNMI KDRNDWVISR
460 470 480 490 500
QRVWGVPIPI FYDNENNPLL DRKLIDHVAS LFEVHGSDIW YEWDVEKLLP
510 520 530 540 550
SDYEGPRDLT KELDIMDVWF DSGTSYNILK RRGLPFPADM YLEGSDQYRG
560 570 580 590 600
WFNSSLTTAI AVDGVSPYKE IVSHGFVLDG KGRKMSKSLG NVIDPLTVMN
610 620 630 640 650
DQGADVLRLW VASVDYEADV RISNDLMKQV SESYRKFRNT FRFMLGVLDG
660 670 680 690 700
FNPEVNYIGW SMRGQLNRVM TDKYYVLATK VNESYAKYNF VEVTRLIIPF
710 720 730 740 750
VVNDLSAFYL DYTKDSLYCD AEDDFERRAI QSTIYDILLG LLRLLTPIMP
760 770 780 790 800
HTTSEAYDSL KYKEYDNIYL EKMPIGGKLK EPKLQENYDI FDELRNHVLK
810 820 830 840 850
HLELAREAKV IGKSLDAHLD LSVDQTTYDA LEYLDLLNKL DKILIVSSVH
860 870 880 890
ITKADQLDIK VSKADGHVCA RCWNIVKEVN QNDVCVRCES VLEGLK
Length:896
Mass (Da):103,532
Last modified:February 5, 2008 - v1
Checksum:i9BBDA617784DDC24
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000896 Genomic DNA Translation: ABX81318.1
RefSeqiWP_012242649.1, NC_010163.1

Genome annotation databases

EnsemblBacteriaiABX81318; ABX81318; ACL_0702
KEGGiacl:ACL_0702

Similar proteinsi

Entry informationi

Entry nameiSYI_ACHLI
AccessioniPrimary (citable) accession number: A9NG38
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: February 5, 2008
Last modified: March 28, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health