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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Acholeplasma laidlawii (strain PG-8A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei543 – 5431Aminoacyl-adenylateUniRule annotation
Binding sitei587 – 5871ATPUniRule annotation
Metal bindingi869 – 8691ZincUniRule annotation
Metal bindingi872 – 8721ZincUniRule annotation
Metal bindingi885 – 8851ZincUniRule annotation
Metal bindingi888 – 8881ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciALAI441768:GI40-702-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:ACL_0702
OrganismiAcholeplasma laidlawii (strain PG-8A)
Taxonomic identifieri441768 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesAcholeplasmatalesAcholeplasmataceaeAcholeplasma
ProteomesiUP000008558 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 896896Isoleucine--tRNA ligasePRO_1000216231Add
BLAST

Proteomic databases

PRIDEiA9NG38.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi441768.ACL_0702.

Structurei

3D structure databases

ProteinModelPortaliA9NG38.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi584 – 5885"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9NG38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYKDTLLMP KTDFEMRGNL GKREPEIQKH WESIDLYNKV LEKNKDNTPF
60 70 80 90 100
VLHDGPPYAN NNIHIGHAFQ KTLKDFVLRY QTMSGRYTPY IPGWDTHGLP
110 120 130 140 150
IENEVTKSGV DRKQLTRAEF RKICREYAIK QVEVQRAQFK RLGILGDWDN
160 170 180 190 200
PYLTLDKSYI ADQVRVFSQM VDKGIIYKGL KPIYWSPSSE SAFAEAEIEY
210 220 230 240 250
MDKQSKSIYV GFDMMGDKFP NTKLLIWTTT PWTLPANLAV SVHPNFDYVW
260 270 280 290 300
FNNDGKNYIA LKELLPKLVE KLGFSHAKVL KEFKGSTLEF MNYKHPLYDR
310 320 330 340 350
VSPIILGEHV TAEDGTGLVH TAPGHGEDDY FVGKKYNLDL LSPVDEKGHM
360 370 380 390 400
TEEAGPYAGM FYEKANAQIV EDLRENGHLL YDETITHSYP HDWRTKKPVI
410 420 430 440 450
FRSTPQWFAS IDMLKGDLLE AIKGVKWHTS WGEVRLTNMI KDRNDWVISR
460 470 480 490 500
QRVWGVPIPI FYDNENNPLL DRKLIDHVAS LFEVHGSDIW YEWDVEKLLP
510 520 530 540 550
SDYEGPRDLT KELDIMDVWF DSGTSYNILK RRGLPFPADM YLEGSDQYRG
560 570 580 590 600
WFNSSLTTAI AVDGVSPYKE IVSHGFVLDG KGRKMSKSLG NVIDPLTVMN
610 620 630 640 650
DQGADVLRLW VASVDYEADV RISNDLMKQV SESYRKFRNT FRFMLGVLDG
660 670 680 690 700
FNPEVNYIGW SMRGQLNRVM TDKYYVLATK VNESYAKYNF VEVTRLIIPF
710 720 730 740 750
VVNDLSAFYL DYTKDSLYCD AEDDFERRAI QSTIYDILLG LLRLLTPIMP
760 770 780 790 800
HTTSEAYDSL KYKEYDNIYL EKMPIGGKLK EPKLQENYDI FDELRNHVLK
810 820 830 840 850
HLELAREAKV IGKSLDAHLD LSVDQTTYDA LEYLDLLNKL DKILIVSSVH
860 870 880 890
ITKADQLDIK VSKADGHVCA RCWNIVKEVN QNDVCVRCES VLEGLK
Length:896
Mass (Da):103,532
Last modified:February 5, 2008 - v1
Checksum:i9BBDA617784DDC24
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000896 Genomic DNA. Translation: ABX81318.1.
RefSeqiWP_012242649.1. NC_010163.1.

Genome annotation databases

EnsemblBacteriaiABX81318; ABX81318; ACL_0702.
KEGGiacl:ACL_0702.
PATRICi20638124. VBIAchLai134690_0682.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000896 Genomic DNA. Translation: ABX81318.1.
RefSeqiWP_012242649.1. NC_010163.1.

3D structure databases

ProteinModelPortaliA9NG38.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi441768.ACL_0702.

Proteomic databases

PRIDEiA9NG38.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABX81318; ABX81318; ACL_0702.
KEGGiacl:ACL_0702.
PATRICi20638124. VBIAchLai134690_0682.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciALAI441768:GI40-702-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PG-8A.

Entry informationi

Entry nameiSYI_ACHLI
AccessioniPrimary (citable) accession number: A9NG38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: February 5, 2008
Last modified: July 22, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.