A9NF46 (SYT_ACHLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	ACL_0354

Organism

Acholeplasma laidlawii (strain PG-8A) [Complete proteome] [HAMAP]

Taxonomic identifier

441768 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Acholeplasma

Protein attributes

Sequence length	642 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00184
Subunit structure	Homodimer By similarity. HAMAP MF_00184
Subcellular location	Cytoplasm By similarity HAMAP MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 642	642	Threonine--tRNA ligase HAMAP MF_00184		PRO_1000077346
Regions
	Region	240 – 539	300	Catalytic HAMAP MF_00184
Sites
	Metal binding	334	1	Zinc; catalytic By similarity
	Metal binding	385	1	Zinc; catalytic By similarity
	Metal binding	516	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9NF46 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 3795831C19379CBD	FASTA	642	75,292
	10 20 30 40 50 60 MIKVTFPDGN IKSYKKGITV LEVAEDISIS LAKKIVAAKF NEDLVEISRK LEHDGKLQLL 70 80 90 100 110 120 TPKEELSFHV LNHSAAHLMA QAIRKLYPDA LFGVGPAIEE GYYYDVDFKD SVFTDADLEE 130 140 150 160 170 180 VEKMMKKLSE ENHLIERIEV SYDEARDIFK NDPYKLELIE VYKDDQLSVY RQGEFIDLCR 190 200 210 220 230 240 GGHVPSTKYI KHFKLLSIAG AYWRGDAKNR QLVRVYGIAY FEKAELDKHL VMLEERKLRD 250 260 270 280 290 300 HRKIGKDLDI FMTSQEVGSG LPFWLPKGAT IRRIIERYIT DKELELGYLH VYTPIMANVE 310 320 330 340 350 360 FYKQSGHWDH YHENMYPPMD LGDGEMLVLR PMNCPHHMMI YKKDIHSYRE LPIRFAELGM 370 380 390 400 410 420 MHRYEKSGAL SGLQRVREMT LNDAHIFVRP DQIKEEFMRV VHLIIEVYKD FKITDYSFRL 430 440 450 460 470 480 SYRDPENKEK YFDDDQMWQR AESELKNVMD EMKLPYKEAI GEAAFYGPKL DIQVRTAMGM 490 500 510 520 530 540 EETLSTVQLD FLLPERFDLT YVGEDGKNNH RPVVIHRGVV STMERFVAYL IEEYKGSFPL 550 560 570 580 590 600 WLAPVQLKLI PVNLDLHKDY VMNLHEQLKK LGFRVESDFR NEKLGYKIRE AQTLKIPYQL 610 620 630 640 VVGDNEMNTH SITYRPYGSE KQINISIDGF IKLLNERMIN KD

References

[1]

"Complete genome and proteome of Acholeplasma laidlawii." Lazarev V.N., Levitskii S.A., Basovskii Y.I., Chukin M.M., Akopian T.A., Vereshchagin V.V., Kostrjukova E.S., Kovaleva G.Y., Kazanov M.D., Malko D.B., Vitreschak A.G., Sernova N.V., Gelfand M.S., Demina I.A., Serebryakova M.V., Galyamina M.A., Vtyurin N.N., Rogov S.I. , Alexeev D.G., Ladygina V.G., Govorun V.M. J. Bacteriol. 193:4943-4953(2011) [PubMed: 21784942] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PG-8A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000896 Genomic DNA. Translation: ABX80976.1.
RefSeq	YP_001620352.1. NC_010163.1.
3D structure databases
ProteinModelPortal	A9NF46.
SMR	A9NF46. Positions 2-641.
ModBase	Search...
Protein-protein interaction databases
STRING	A9NF46.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5804340.
GenomeReviews	Gene locus ACL_0354 in contig CP000896_GR.
KEGG	acl:ACL_0354.
PATRIC	20637428. VBIAchLai134690_0338.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG352811.
OMA	GRKWQLG.
Enzyme and pathway databases
BioCyc	ALAI441768:ACL_0354-MONOMER.
Family and domain databases
HAMAP	MF_00184. Thr_tRNA_synth. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR012675. Beta-grasp_ferredoxin-type. IPR004095. TGS. IPR012676. TGS-like. IPR002320. Thr-tRNA-synth_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
KO	K01868.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF81271. TGS-like. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. ThrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_ACHLI

Accession

Primary (citable) accession number: A9NF46

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program