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A9NET8 (SYL_ACHLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:ACL_0242
OrganismAcholeplasma laidlawii (strain PG-8A) [Complete proteome] [HAMAP]
Taxonomic identifier441768 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesAcholeplasmatalesAcholeplasmataceaeAcholeplasma

Protein attributes

Sequence length803 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 803803Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334722

Regions

Motif41 – 5212"HIGH" region HAMAP-Rule MF_00049
Motif581 – 5855"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5841ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A9NET8 [UniParc].

Last modified February 5, 2008. Version 1.
Checksum: 4542359C0C2E5190

FASTA80392,810
        10         20         30         40         50         60 
MHKYEHRKIE EKWQKHWLDN KLFKTKNDRF HQKYYVLDMF PYPSASGLHV GHIEGYTATD 

        70         80         90        100        110        120 
IVSRFKRMQG YNVLHPMGWD AYGLPSEQYA LATGKDPKSF TYQNISNFKR QIIEMGKGVD 

       130        140        150        160        170        180 
WDKELATTDP EYFKWTQWIF KRLYEKGLAV LKDVEVNWCE GLGTVLANDE IEIINGQMVS 

       190        200        210        220        230        240 
ERGHYPVIKK PMRQWVLRIT EYADRLLDDL ALVDWPENLK EMQRNWIGKS SGAVIEFEVA 

       250        260        270        280        290        300 
STDYKFEIFT TRPDTIYGVT YCVLSPEHPL VEKIVTQDEE KDVLRYIEQT KMKSDLDRIA 

       310        320        330        340        350        360 
DKSKTGVFTG AFAKHPLTNQ LIPIWIGDYV LPQYGMGAVM AVPAHDDRDY EFAMQYGLNI 

       370        380        390        400        410        420 
IEVIKGGSEG AYTGDGIHHH SGIIDGLYNE EAIEKITKHL EDRKKGYKHY TYKLRDWVFS 

       430        440        450        460        470        480 
RQRYWGEPFP VIYDEDGQIH LVPDDELPLE LPYLEFIKPS GTGESPLANA RDWLYVDING 

       490        500        510        520        530        540 
KKGRRDTNTM PQLAGSSWYY IGYILKSNLG YIPLDSKEAK AVLDQFLPVD LYVGGTEHAV 

       550        560        570        580        590        600 
GHLLYARFWH KFFYDLGFVS SKEPFMKLVN QGMILGADHS KMSKSKGNSV SPDDFIQSHG 

       610        620        630        640        650        660 
ADALRLFEMF MGPLEAEKPW SNEGLEGAKR FLDRVWRMFD FEITQQPVDA LQTVYHQTVK 

       670        680        690        700        710        720 
KVTEDYEKLA FNTAISQMMI FVNEVYKTKV IGAAQARGFL KLLNPIAPHL TEEINETVLK 

       730        740        750        760        770        780 
HHEDLVYSEW PTYDESFLIE TEVEVAIQVN GKLRGRLQVK KDMVEEDLKK LALEHENVIK 

       790        800 
HVEGLTIVKV IVIPNKIVNI VVR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000896 Genomic DNA. Translation: ABX80868.1.
RefSeqYP_001620244.1. NC_010163.1.

3D structure databases

ProteinModelPortalA9NET8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING441768.ACL_0242.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABX80868; ABX80868; ACL_0242.
GeneID5804115.
KEGGacl:ACL_0242.
PATRIC20637194. VBIAchLai134690_0224.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycALAI441768:GI40-242-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYL_ACHLI
AccessionPrimary (citable) accession number: A9NET8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 5, 2008
Last modified: April 16, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries