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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Acholeplasma laidlawii (strain PG-8A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei217ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciALAI441768:G1GAM-140-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:ACL_0139
OrganismiAcholeplasma laidlawii (strain PG-8A)
Taxonomic identifieri441768 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesAcholeplasmatalesAcholeplasmataceaeAcholeplasma
Proteomesi
  • UP000008558 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000743151 – 449Glutamate--tRNA ligaseAdd BLAST449

Proteomic databases

PRIDEiA9NEI5

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi441768.ACL_0139

Structurei

3D structure databases

ProteinModelPortaliA9NEI5
SMRiA9NEI5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi10 – 20"HIGH" regionUniRule annotationAdd BLAST11
Motifi214 – 218"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252720
KOiK01885
OMAiHYINTLP
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 2 hits
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 2 hits
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A9NEI5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKFRARYAP SPTGHLHIGN ARTALFNYLF ARHHGGDFII RIEDTDVARN
60 70 80 90 100
VEGGITSQLN NLKWLGMDWD EGVDVGGSFG PYNQLSRLEL YKKYAFELLE
110 120 130 140 150
KGYAYKDFKE GSEDFAIRFK VPENVLYEFD DVIRGTLKFE SKDVEDWIIL
160 170 180 190 200
KDNGIPTYNF AVVIDDHYME ITHVFRGEEH ITNTPKQLMV YDALGWEYPT
210 220 230 240 250
FGHMTIIVNE DRKKLSKRDT NTIQFIEDYK NLGFLPEAML NFLSLLGWSP
260 270 280 290 300
KDDEEILSKE ELISLFDEHR LSAAPSYFDK QKLAYINSRY LKALSMDELK
310 320 330 340 350
DLTRPFLINH GIEIKNEAWL ESLLSILKDR LSYGAEITKY YDQFFHHDFV
360 370 380 390 400
LEPAVLEEIK EFDNEVVIKG FMGAISSVDF TDDVAINQAL KDTGKALNIK
410 420 430 440
GKPLFMPIRI ATTGEAHGPS LPVSLSLLGK ELVIKRMNKT LEVLKGETK
Length:449
Mass (Da):51,597
Last modified:February 5, 2008 - v1
Checksum:i3FBF9203BDA4D10D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000896 Genomic DNA Translation: ABX80765.1
RefSeqiWP_012242096.1, NC_010163.1

Genome annotation databases

EnsemblBacteriaiABX80765; ABX80765; ACL_0139
KEGGiacl:ACL_0139

Similar proteinsi

Entry informationi

Entry nameiSYE_ACHLI
AccessioniPrimary (citable) accession number: A9NEI5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 5, 2008
Last modified: May 23, 2018
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
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