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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Acholeplasma laidlawii (strain PG-8A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei217ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:ACL_0139
OrganismiAcholeplasma laidlawii (strain PG-8A)
Taxonomic identifieri441768 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesAcholeplasmatalesAcholeplasmataceaeAcholeplasma
Proteomesi
  • UP000008558 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000743151 – 449Glutamate--tRNA ligaseAdd BLAST449

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi441768.ACL_0139.

Structurei

3D structure databases

ProteinModelPortaliA9NEI5.
SMRiA9NEI5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi10 – 20"HIGH" regionAdd BLAST11
Motifi214 – 218"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20. Bacteria.
COG0008. LUCA.
HOGENOMiHOG000252720.
KOiK01885.
OMAiNWINSQY.
OrthoDBiPOG091H021W.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B. 1 hit.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 2 hits.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9NEI5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKFRARYAP SPTGHLHIGN ARTALFNYLF ARHHGGDFII RIEDTDVARN
60 70 80 90 100
VEGGITSQLN NLKWLGMDWD EGVDVGGSFG PYNQLSRLEL YKKYAFELLE
110 120 130 140 150
KGYAYKDFKE GSEDFAIRFK VPENVLYEFD DVIRGTLKFE SKDVEDWIIL
160 170 180 190 200
KDNGIPTYNF AVVIDDHYME ITHVFRGEEH ITNTPKQLMV YDALGWEYPT
210 220 230 240 250
FGHMTIIVNE DRKKLSKRDT NTIQFIEDYK NLGFLPEAML NFLSLLGWSP
260 270 280 290 300
KDDEEILSKE ELISLFDEHR LSAAPSYFDK QKLAYINSRY LKALSMDELK
310 320 330 340 350
DLTRPFLINH GIEIKNEAWL ESLLSILKDR LSYGAEITKY YDQFFHHDFV
360 370 380 390 400
LEPAVLEEIK EFDNEVVIKG FMGAISSVDF TDDVAINQAL KDTGKALNIK
410 420 430 440
GKPLFMPIRI ATTGEAHGPS LPVSLSLLGK ELVIKRMNKT LEVLKGETK
Length:449
Mass (Da):51,597
Last modified:February 5, 2008 - v1
Checksum:i3FBF9203BDA4D10D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000896 Genomic DNA. Translation: ABX80765.1.
RefSeqiWP_012242096.1. NC_010163.1.

Genome annotation databases

EnsemblBacteriaiABX80765; ABX80765; ACL_0139.
KEGGiacl:ACL_0139.
PATRICi20636992. VBIAchLai134690_0123.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000896 Genomic DNA. Translation: ABX80765.1.
RefSeqiWP_012242096.1. NC_010163.1.

3D structure databases

ProteinModelPortaliA9NEI5.
SMRiA9NEI5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi441768.ACL_0139.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABX80765; ABX80765; ACL_0139.
KEGGiacl:ACL_0139.
PATRICi20636992. VBIAchLai134690_0123.

Phylogenomic databases

eggNOGiENOG4105C20. Bacteria.
COG0008. LUCA.
HOGENOMiHOG000252720.
KOiK01885.
OMAiNWINSQY.
OrthoDBiPOG091H021W.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B. 1 hit.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 2 hits.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYE_ACHLI
AccessioniPrimary (citable) accession number: A9NEI5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 5, 2008
Last modified: November 30, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.