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A9NEA9 (GLYA_ACHLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pyridoxal-phosphate-dependent serine hydroxymethyltransferase
Short name=SHMT
Short name=Serine methylase
EC=2.1.2.1
Gene names
Name:glyA
Ordered Locus Names:ACL_0047
OrganismAcholeplasma laidlawii (strain PG-8A) [Complete proteome] [HAMAP]
Taxonomic identifier441768 [NCBI]
Taxonomic lineageBacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Acholeplasma

Protein attributes

Sequence length409 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate serving as the one-carbon carrier By similarity.

Catalytic activity

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051

Cofactor

Pyridoxal phosphate By similarity. HAMAP MF_00051

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051

Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity HAMAP MF_00051.

Sequence similarities

Belongs to the SHMT family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 409409Pyridoxal-phosphate-dependent serine hydroxymethyltransferase
PRO_0000369893

Regions

Region120 – 1223Substrate binding By similarity

Sites

Binding site301Pyridoxal phosphate By similarity
Binding site501Pyridoxal phosphate By similarity
Binding site521Substrate By similarity
Binding site591Substrate binding By similarity
Binding site601Pyridoxal phosphate By similarity
Binding site941Pyridoxal phosphate By similarity
Binding site1161Substrate By similarity
Binding site1711Pyridoxal phosphate By similarity
Binding site1991Pyridoxal phosphate By similarity
Binding site2241Pyridoxal phosphate By similarity
Binding site2311Pyridoxal phosphate By similarity
Binding site2571Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen By similarity
Binding site3581Pyridoxal phosphate By similarity

Amino acid modifications

Modified residue2251N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
A9NEA9 [UniParc].

Last modified February 5, 2008. Version 1.
Checksum: 6CD1BBA4336C4CB2

FASTA40945,324
        10         20         30         40         50         60 
MTLKDYDLEL FNAIQREDNR QKEHIELIAS ENFVSDAVLE AQGSILTNKY AEGYPNKRYY 

        70         80         90        100        110        120 
GGCEFVDQVE ILAQDRLKQI FNAKFVNVQP HSGSQANAAV YQALLSPGDR VLGMDLNAGG 

       130        140        150        160        170        180 
HLTHGYKLSF SGHYYEAHAY GVSRFDERID YEEVLKIAIE VKPKMIIAGA SAYPRVIDFK 

       190        200        210        220        230        240 
KFREIADTVG AYLFVDMAHI AGLVACGLHP SPLPYADVVT STTHKTLRGP RGGIILTNDA 

       250        260        270        280        290        300 
SIAKKIDRAV FPGQQGGPLM HIIAAKAVAF KEALDPNFKV YQTQVIKNAK ALSDTFKSLG 

       310        320        330        340        350        360 
YKLISDGTDN HLILVDVKSK LGITGRDAED ALYKANITIN KNQLPFDQEK PMLTSGIRLG 

       370        380        390        400 
TPAMTTKGFK ENEFIKVAQL IDEVLSNINN EEVINKVKKE VLKLMKDVK

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000896 Genomic DNA. Translation: ABX80689.1.
RefSeqYP_001620065.1. NC_010163.1.

3D structure databases

ProteinModelPortalA9NEA9.
SMRA9NEA9. Positions 7-402.
ModBaseSearch...

Protein-protein interaction databases

STRINGA9NEA9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5803954.
GenomeReviewsGene locus ACL_0047 in contig CP000896_GR.
KEGGacl:ACL_0047.
PATRIC20636812. VBIAchLai134690_0048.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG301263.
OMAYKANITI.

Enzyme and pathway databases

BioCycALAI441768:ACL_0047-MONOMER.

Family and domain databases

HAMAPMF_00051. SHMT.
[Tree]
InterProIPR015424. PyrdxlP-dep_Trfase_major_dom.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
Gene3DG3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KOK00600.
PANTHERPTHR11680. Gly_HO-Metrfase. 1 hit.
PfamPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFPIRSF000412. SHMT. 1 hit.
SUPFAMSSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITEPS00096. SHMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLYA_ACHLI
AccessionPrimary (citable) accession number: A9NEA9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 5, 2008
Last modified: January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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