Reviewed, UniProtKB/Swiss-Prot A9NEA9 (GLYA_ACHLI)

Names and origin

Protein names

Recommended name:
    Serine hydroxymethyltransferase
      Short name=Serine methylase
      Short name=SHMT
    EC=2.1.2.1

Gene names

Name:	glyA
Ordered Locus Names:	ACL_0047

Organism

Acholeplasma laidlawii (strain PG-8A) [Complete proteome] [HAMAP]

Taxonomic identifier

441768 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Acholeplasma

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interconversion of serine and glycine By similarity. HAMAP MF_00051
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_00051
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051
Subunit structure	Homotetramer By similarity. HAMAP MF_00051
Subcellular location	Cytoplasm By similarity HAMAP MF_00051.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 409	409	Serine hydroxymethyltransferase HAMAP MF_00051		PRO_0000369893
Amino acid modifications
	Modified residue	225	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9NEA9-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 6CD1BBA4336C4CB2	FASTA	409	45,324
	10 20 30 40 50 60 MTLKDYDLEL FNAIQREDNR QKEHIELIAS ENFVSDAVLE AQGSILTNKY AEGYPNKRYY 70 80 90 100 110 120 GGCEFVDQVE ILAQDRLKQI FNAKFVNVQP HSGSQANAAV YQALLSPGDR VLGMDLNAGG 130 140 150 160 170 180 HLTHGYKLSF SGHYYEAHAY GVSRFDERID YEEVLKIAIE VKPKMIIAGA SAYPRVIDFK 190 200 210 220 230 240 KFREIADTVG AYLFVDMAHI AGLVACGLHP SPLPYADVVT STTHKTLRGP RGGIILTNDA 250 260 270 280 290 300 SIAKKIDRAV FPGQQGGPLM HIIAAKAVAF KEALDPNFKV YQTQVIKNAK ALSDTFKSLG 310 320 330 340 350 360 YKLISDGTDN HLILVDVKSK LGITGRDAED ALYKANITIN KNQLPFDQEK PMLTSGIRLG 370 380 390 400 TPAMTTKGFK ENEFIKVAQL IDEVLSNINN EEVINKVKKE VLKLMKDVK

References

[1]

"Acholeplasma laidlawii complete genome." Kovaleva G.Y., Kazanov M.D., Malko D.B., Vitreschak A.G., Sernova N.V., Gelfand M.S., Lazarev V.N., Levitskii S.A., Basovskii Y.I., Chukin M.M., Akopian T.A., Vereshchagin V.A., Kostrjukova E.S., Selezneva O.V., Vtyurin N.N., Rogov S.I., Alekseev D.G., Ladygina V.G. , Titova G.A., Karpov V.A., Govorun V.M. Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000896 Genomic DNA. Translation: ABX80689.1.
RefSeq	YP_001620065.1.
3D structure databases
SMR	A9NEA9. Positions 7-402.
ModBase	Search...
Genome annotation databases
GeneID	5803954.
GenomeReviews	Gene locus ACL_0047 in contig CP000896_GR.
KEGG	acl:ACL_0047.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG301263.
OMA	YKANITI.
Family and domain databases
HAMAP	MF_00051_B. SHMT_B. [Tree]
InterPro	IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA_ACHLI

Accession

Primary (citable) accession number: A9NEA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 5, 2008
Last modified:	January 19, 2010
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)