A9NE95 (FTHS_ACHLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	ACL_0032

Organism

Acholeplasma laidlawii (strain PG-8A) [Complete proteome] [HAMAP]

Taxonomic identifier

441768 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Acholeplasma

Protein attributes

Sequence length	527 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 527	527	Formate--tetrahydrofolate ligase HAMAP MF_01543		PRO_0000333311
Regions
	Nucleotide binding	53 – 60	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9NE95 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 7C37DFF8C7435489	FASTA	527	58,201
	10 20 30 40 50 60 MNTYNYLIDE LHILDDEIIS YGKDKFKIEL SLQERLKDKA PGKLILVTSI NPTSSGEGKT 70 80 90 100 110 120 TLSIGLAQGF KKNGKDVMLA LREPSMGPVF GMKGGATGGG VSILEPSLDI DLHFNGDIHA 130 140 150 160 170 180 LTSANNLLSA IIDNHMYFGN ELNIKDVYWQ RALDVNDRSL REVKTKARDD KFTITAASEM 190 200 210 220 230 240 MAILALARDF KDLKERLNNI LIGTDKDGKD LFVSDLKCAD SLALLLKDAI KPNLVFAKEM 250 260 270 280 290 300 VPALVHAGPF ANIAHGCNSV IATNTALKLA DYVITEAGFG ADLGMEKFLH IKQPHLYTKA 310 320 330 340 350 360 SVVVVVATIK ALKLHGGVTE SNLDEPNIEA LSKGLENIEK HLENIKLFGL NSVVALNKFD 370 380 390 400 410 420 TDSEEELQFL KNWARINHLN YGISEGYSKG GEGTKDLAKL VEKVAYEPSK FKRIYSNEEN 430 440 450 460 470 480 HEYKIRKIAE NIYGAKDVIF SQQAKKKLNQ YKHLEIPICI AKTPLSLSGD PKLKGRPRDF 490 500 510 520 VLEISDIKVS LGANLLVVLT KGINTMPGLN NRPRALDFKL DDKGELI

References

[1]

"Complete genome and proteome of Acholeplasma laidlawii." Lazarev V.N., Levitskii S.A., Basovskii Y.I., Chukin M.M., Akopian T.A., Vereshchagin V.V., Kostrjukova E.S., Kovaleva G.Y., Kazanov M.D., Malko D.B., Vitreschak A.G., Sernova N.V., Gelfand M.S., Demina I.A., Serebryakova M.V., Galyamina M.A., Vtyurin N.N., Rogov S.I. , Alexeev D.G., Ladygina V.G., Govorun V.M. J. Bacteriol. 193:4943-4953(2011) [PubMed: 21784942] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PG-8A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000896 Genomic DNA. Translation: ABX80675.1.
RefSeq	YP_001620051.1. NC_010163.1.
3D structure databases
ProteinModelPortal	A9NE95.
ModBase	Search...
Protein-protein interaction databases
STRING	A9NE95.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5803464.
GenomeReviews	Gene locus ACL_0032 in contig CP000896_GR.
KEGG	acl:ACL_0032.
PATRIC	20636782. VBIAchLai134690_0033.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG677721.
OMA	EIMAVLC.
Enzyme and pathway databases
BioCyc	ALAI441768:ACL_0032-MONOMER.
Family and domain databases
HAMAP	MF_01543. FTHFS. [Tree]
InterPro	IPR000559. Formate_THF_ligase. [Graphical view]
KO	K01938.
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. False negative. PS00722. FTHFS_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_ACHLI

Accession

Primary (citable) accession number: A9NE95

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program