A9NE58 (SYN_ACHLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Asparagine--tRNA ligase
EC=6.1.1.22

Alternative name(s):
Asparaginyl-tRNA synthetase
Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	ACL_1427

Organism

Acholeplasma laidlawii (strain PG-8A) [Complete proteome] [HAMAP]

Taxonomic identifier

441768 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Acholeplasma

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm By similarity HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Asparagine--tRNA ligase HAMAP MF_00534		PRO_1000081843

Sequences

	Sequence		Length	Mass (Da)
	A9NE58 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: EFC408E65F86A995	FASTA	463	53,388
	10 20 30 40 50 60 MDLTIRTIYK DPARYAGQEV ILKGWIRNHR DQKEFGFINL NDGSFFENIQ VVYERATTKD 70 80 90 100 110 120 FDAVTRLSVG ASIEVIGTLV LTPDRSQPFE IKALEVRLIG DSGENYPIQP KRHTREFLRE 130 140 150 160 170 180 VAHLRPRTNL FSAVFRVRSI AAYAIHKFFQ EQDFVYVHTP ILTANDGEGA GAMFKVTTLD 190 200 210 220 230 240 MNKPPRNEDS SIDYSKDFFG REANLTVTGQ LEGEAYAHAF RNIYTFGPTF RAENSNTTTH 250 260 270 280 290 300 ASEFWMIEPE IAFADLNDNM ELIERMVKYV TSYVLEHAKE EMAFFDKFVE KGLLTKLDNL 310 320 330 340 350 360 LKSSFKRITH EEAINILLKS NHKFENQPKH GEDLAKEHER YLTEKAFHGP VFVKDWPKDI 370 380 390 400 410 420 KAFYMRLNDD QKTVAAVDLL VPGSGELVGG SQREERLDVL LKRMEDMHVP VKDLDWYLDL 430 440 450 460 RRYGGVQTSG FGLGFERYLI YVTGVENIRD VIPFPRTPKN ILF

References

[1]

"Complete genome and proteome of Acholeplasma laidlawii." Lazarev V.N., Levitskii S.A., Basovskii Y.I., Chukin M.M., Akopian T.A., Vereshchagin V.V., Kostrjukova E.S., Kovaleva G.Y., Kazanov M.D., Malko D.B., Vitreschak A.G., Sernova N.V., Gelfand M.S., Demina I.A., Serebryakova M.V., Galyamina M.A., Vtyurin N.N., Rogov S.I. , Alexeev D.G., Ladygina V.G., Govorun V.M. J. Bacteriol. 193:4943-4953(2011) [PubMed: 21784942] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PG-8A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000896 Genomic DNA. Translation: ABX82018.1.
RefSeq	YP_001621394.1. NC_010163.1.
3D structure databases
ProteinModelPortal	A9NE58.
ModBase	Search...
Protein-protein interaction databases
STRING	A9NE58.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5803367.
GenomeReviews	Gene locus ACL_1427 in contig CP000896_GR.
KEGG	acl:ACL_1427.
PATRIC	20639544. VBIAchLai134690_1372.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG745843.
OMA	AIHRFFH.
ProtClustDB	PRK03932.
Enzyme and pathway databases
BioCyc	ALAI441768:ACL_1427-MONOMER.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01893.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF6. PTHR22594:SF6. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00457. AsnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_ACHLI

Accession

Primary (citable) accession number: A9NE58

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program