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A9NDX8

- LIPA_COXBR

UniProt

A9NDX8 - LIPA_COXBR

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Protein

Lipoyl synthase

Gene

lipA

Organism
Coxiella burnetii (strain RSA 331 / Henzerling II)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi62 – 621Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi67 – 671Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi73 – 731Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi88 – 881Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi92 – 921Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi95 – 951Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCBUR360115:GI0X-1419-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-synUniRule annotation
Short name:
LSUniRule annotation
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Sulfur insertion protein LipAUniRule annotation
Gene namesi
Name:lipAUniRule annotation
Ordered Locus Names:COXBURSA331_A1414
OrganismiCoxiella burnetii (strain RSA 331 / Henzerling II)
Taxonomic identifieri360115 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
ProteomesiUP000008557: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Lipoyl synthasePRO_1000077954Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi360115.COXBURSA331_A1414.

Structurei

3D structure databases

ProteinModelPortaliA9NDX8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

A9NDX8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTYDPSQKS LGKEKLSRIP VKIEATHTPL RKPDWIRIRL STDSKVSQLK
60 70 80 90 100
KLLRENHLVT VCEEASCPNL NECFGHGTAT FMIMGDKCTR RCSFCDVGHG
110 120 130 140 150
RPDPLDPEEP VNLANTVSIM SLRYVVITSV DRDDLRDGGA QHYAQCINAV
160 170 180 190 200
REKNPGIKVE VLVPDFRGRM EKALDQLAQG LPDVFNHNIE TAPRLYKQAR
210 220 230 240 250
PGADYPWSLA LLQTFKKRFP GIPTKSGMML GLGETREEVE MVMRDLRQHE
260 270 280 290 300
VDRLTLGQYL QPTRYHMPVD RYVTPQEFQE LGELAKKLGF SNVASGPLVR
310
SSYHADLQAQ GERVS
Length:315
Mass (Da):35,604
Last modified:February 5, 2008 - v1
Checksum:i9DC26837BAD4EF80
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000890 Genomic DNA. Translation: ABX77392.1.
RefSeqiYP_001597118.1. NC_010117.1.

Genome annotation databases

EnsemblBacteriaiABX77392; ABX77392; COXBURSA331_A1414.
GeneIDi5794068.
KEGGicbs:COXBURSA331_A1414.
PATRICi17927105. VBICoxBur33747_1419.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000890 Genomic DNA. Translation: ABX77392.1 .
RefSeqi YP_001597118.1. NC_010117.1.

3D structure databases

ProteinModelPortali A9NDX8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 360115.COXBURSA331_A1414.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABX77392 ; ABX77392 ; COXBURSA331_A1414 .
GeneIDi 5794068.
KEGGi cbs:COXBURSA331_A1414.
PATRICi 17927105. VBICoxBur33747_1419.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci CBUR360115:GI0X-1419-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequencing of phylogenetically and phenotypically diverse Coxiella burnetii isolates."
    Seshadri R., Samuel J.E.
    Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RSA 331 / Henzerling II.

Entry informationi

Entry nameiLIPA_COXBR
AccessioniPrimary (citable) accession number: A9NDX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 5, 2008
Last modified: October 29, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3